Joey Tidei

Microtubules 🟢 protruding into the dynamic actin 🟣 network of a neuronal growth cone in DIV2 hippocampal neurons. @spirochrome.com SiR-Tubulin and SPY-FastActX working wonders on this #FluorescenceFriday

LIM Domain Proteins link molecular and global tension by recognizing strained actin in adhesions Mechanotransduction is fundamental to cell signaling and depends on force-sensitive adhesion proteins. How these proteins differentiate and integrate their responses to tension remains an open questio...

Our new preprint is out! Led by @stefanosala89.bsky.social sky.social we investigate the difference between molecular tension sensing and global tension on the focal adhesion. Follow along for a thread of our findings:
www.biorxiv.org/content/10.1...

A day ahead of #FluorescenceFriday, a (temporal) switch from the bulb to the cortex.

🟣 NeuN
🔵 AnkG

🔬🧠🧪

Imaging neurons quickly for 3min typically won't give you huge cell-wide cytoskeletal events, but you do get to capture fun little tugs and loops in the soma and neurites (as well as whatever the hell is going on in the bottom right).

Stain: @spirochrome.com SiR-Tubulin

Happy #FlourescenceFriday!

Myosin 2 – A general contractor for the cytoskeleton Cells derive their shape, and in turn much of their behavior, from the organization of the cytoskeleton. While a myriad of proteins contribute to the …

Did you wake up this morning and think "Huh, I'd love to learn more about how myosin 2 filaments assemble and their contribution to cell architecture and function"? What a coincidence, I've got just the review for you.

Our PhD student Louis Romette is optimizing long-term live-cell imaging. He just casually dropped this 65-hour movie of a growing neuron (div 3 to 6) with knocked-in actin 🔥🕶️ #realtimemicroscopy

Beautiful! What imaging media did you wind up going with?

Two months left for FASEB Conference - Septins: Spatial regulators of cell biology
Register now:
events.faseb.org/event/septin...
Come find out what these filaments do at the interface of the actin and microtubule cytoskeletons and cell membranes. You'll be hooked for life.

Thank you @daniellebeckman.bsky.social ! Obviously the same for all of your images!

Congrats to the NeuroArt Grand Prize winners!
🥇 Totally Tubular –Joey Tidei @j-today.bsky.social
🥈 Neurosphere – Adam Soos
🥉 Unwelcome Guest – Danielle Beckman @daniellebeckman.bsky.social

Thanks to all who shared their art! Want to be next? Enter our contest! 🔗 neuroart.com/annual-grand...

For this #FluorescenceFriday I share another example of the possibilities of making art with imageJ processing. This is a 3 day long time-lapse of a growing slime mold, with 15 min growth waves color coded.
the white things are oat flakes. I'll share the original video in comments

#Sciart

Almost forgot to mention.... @kwolbachia.bsky.social Citynight LUT strikes again!

I've been lacking on #FluorescenceFriday the past month or two, but winning the annual MBF NeuroArt competition was a lovely surprise to end the week. Thank you @mbfbioscience.bsky.social for the recognition!

neuroart.com/annual-grand...

Unfortunately it is. Happy to see ideas being thrown around here though lol

I do most of my troubleshooting imaging with NBM/B27 but if I need good clear imaging I would use NEUMO/SOS. I'll have to check but I believe the longest I did with that media was overnight and it was definitely reduced autofluorescence. Relatively new, now relatively old I guess.

Following since I've just been informed NEUMO/SOS is discontinued 😧

The world desperately needs more microscope merch. I’d spend half my stipend.

Zoomed in on the base of an axon looking at some funky actin dynamics for this #FluorescenceFriday

I’ve never had luck with warm proofs. Cold proof overnight always gives me great spring 👍

#TIRFSIMThursday with a COS cell labeled for actin, clathrin and microtubules

It'll be a slightly smaller crew than previous years but we'll still be at #cellbio24 #ASCB! If you like LIM proteins or septins come check out our posters:

This place really is bumpin’… My first #FluorescenceFriday on Bluesky. Recycled, but a DIV4 rat hippocampal neuron expressing a probe for F-Actin and imaged on an LSM880 with Airyscan.

Hi everyone! Kicking off my first #FluorescenceFriday here with a 3D SIM image of actin, and of course had to go with my favorite blue LUT (JDM Duo Intense Cyan) for Bluesky.