R/Proteomics

New publication alert! 📢

We collaborated with @jesperolsenlab.bsky.social to comprehensively characterize LysC homologs and study their impact on proteomic experiments.

For challenging samples, adding LysC can definitely make a difference in the data quality.

Congrats to all authors!

#proteomics

Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows This study presents a comparative analysis of three LysC endopeptidase homologues from Achromobacter lyticus (A. lyticus),Pseudomonas aeruginosa and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture workflow with HeLa cell lysates, we assessed the enzymes’ cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three LysC homologues exhibited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with superior peptide identification, digestion efficiency, and protein coverage, especially at shorter digestion times. Our experiments using a combination ofA. lyticusLysC and trypsin demonstrated the importance of employing LysC for significantly minimizing missed cleavage rates in tryptic digests, especially with regard to lysine-containing peptides. This study underscores A. lyticus LysC’s potential as an optimal choice for enhancing mass spectrometry-based proteomics.

Does enzyme quality matter in #proteomics?

New study in JPR w/ @jesperolsenlab.bsky.social shows: LysC-Trypsin reduces missed cleavages from 30% → 15%

KPL's A. lyticus LysC achieves >90% efficiency in systematic comparison.

Congrats to the authors!
pubs.acs.org/doi/10.1021/...
#TeamMassSpec

SCIEX or Thermo for Proteomics Posted in r/proteomics by u/Expensive-Painter-18 • 4 points and 1 comment

Take a break from holiday doom scrolling and help somebody decide on which MS instrument to purchase for #proteomics! Specifically, one user is requesting input to decide between SCIEX or Thermo for proteomic MS instrument.

old.reddit.com/r/proteomics...

#Teammassspec

From the proteomics community on Reddit Explore this post and more from the proteomics community

Best method for RELATIVE protein abundance from #proteomics experiments?? 🤔🤔🤔

#TeamMassSpec

www.reddit.com/r/proteomics...

Mass Spec

Finally, we're looking for someone to join our #MassSpec Core Facility to support all the protein/peptide MS & development needs of our research groups utilising:

🧪Protein chemistry
📈Liquid chromatography
⚛️Tandem mass spectrometry
🖥️Data analysis

More at:
www.cruk.manchester.ac.uk/career/mi-25...

(Huber lab)[EMBL] welcomes applications for a PhD in ML for spatial omics [representation learning + integration with biostatistics, cell + anatomy foundation models, collaborate with domain scientists on cancer and dev:bio discovery science] |> Apply though the ELLIS portal ::: Deadline 2025-10-31

From the proteomics community on Reddit Explore this post and more from the proteomics community

#proteomics choose your fighter!

www.reddit.com/r/proteomics...

Shotgun Proteomics on FFPE blocks? I'm looking for your experience on doing proteomics on FFPE samples of tissues. Could you share protocols you use and tips and tricks? Thank...

old.reddit.com/r/proteomics...

User interested to hear from fellow #proteomics and #TeamMassSpec researchers their experience with spatial proteomics on FFPE blocks.

Identitätsüberprüfung eines aufgereinigten Proteins **Hallo liebe Biowissenschaftler:innen 😊** könntet ihr mir bitte bei der Vorbereitung auf mein Praktikum helfen? Ich bräuchte...

Identitätsüberprüfung eines aufgereinigten Proteins ?

#Proteomics #TeamMassSpec

old.reddit.com/r/proteomics...

We're here!! Aaaand we need some help :) Calling all #proteomics researchers

www.reddit.com/r/proteomics...