@huiskenlab.bsky.social
High-end modular and portable #lightsheet microscopy for inside and outside the lab / Flamingo project / Imaging development, physiology and anatomy in live specimens and cleared tissues. Former @morgridgeinstitute.bsky.social, @mpi-cbg.de huiskenlab.com
We advocate for model-guided, representative sampling and visualizations that make biological variation tangible.
New paper published! We explore how modern microscopy transforms biology from observing single examples to navigating distributions of phenotypes.
doi.org/10.3389/fbin...
#lightsheet #SmartMicroscopy #BiologicalVariation #visualization #RepresentativeSampling
New Huisken lab member: We’re excited to welcome Roman Tsukanov (@romantsukanov.bsky.social)! With expertise in super-resolution microscopy using DNA-PAINT, Roman will combine this technology with light sheet fluorescence microscopy. Exciting times ahead!
#SuperResolution #DNApaint #lightsheet #LSFM
Lichtblattmikroskope erstellen 3D-Scans von Geweben und Organen – erzeugen bei großen Proben aber unscharfe Bilder. Forschende haben eine Plattform entwickelt, die diese Hürde überwindet. Mehr erfahren: www.uni-goettingen.de/de/3240.html...
#MBexC #UMG @mbexc.bsky.social @huiskenlab.bsky.social
Enjoying a Glühwein at the Christmas market - yearly Huisken lab tradition 🎄
#Mastodon, the Command Center for Large-Scale Lineage-Tracing Microscopy Datasets, is finally heading for publication (well, let's see). To #cite or not to cite shall no longer be the question 🙃, because the #preprint. Software is available in every #Fiji near you.
www.biorxiv.org/content/10.6...
We’re collecting protocols and reviews for studying neural mechanisms in marine invertebrates. Submit your functional imaging, connectomics, or electrophysiology techniques to our JOVE Methods Collection and help make these approaches accessible across species. app.jove.com/methods-coll...
This work is nicely summarised by @cppape.bsky.social:
bsky.app/profile/cppa...
In a new preprint, our cochlea imaging data is used to train CochleaNet, a new machine-learning model from Pape and Moser's labs that segments and analyzes entire spiral ganglion neurons, inner hair cells, and synapses — a step toward truly quantitative cochlear biology.
tinyurl.com/CochleaNet
Studying cochlear neuroanatomy demands imaging the entire cochlea at subcellular resolution. In our recent study (tinyurl.com/isotropicLSFM), we recorded the cochlea at isotropic resolution across the whole organ, laying the groundwork for what follows…
#lightsheet
#isotropic
#cochlea
🧬 10 @dfg.de-funded PhD positions at @goettingen-campus.de 🧬
❗Focus: Evolution of gene regulatory networks & Wnt signaling in emerging models (insects, snails, flatworms, cnidarians).
💻 Methods: 'Omics, Bioinformatics, CRISPR/RNAi & Imaging.
📌Start: June 2026.
#EvoDevo #Genomics #Bioinformatics
Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue - @huiskenlab.bsky.social go.nature.com/4odcS49
The same microscope can image larger samples, including a whole mouse brain (1 cm^3), and supports hybrid low- and high-resolution modes. Low-resolution imaging gives a full overview, while high-resolution mode enables multi-ROI isotropic submicron imaging on the same specimen.
The system also supports larger samples at higher RI (e.g., 1.56), such as mouse cochlea, which are dipped directly into immersion media to image neurites and reconstruct individual connections between spiral ganglion neurons and inner hair cells across the entire cochlea.
The microscope can image fragile samples like cleared zebrafish, RI-matched to 1.47 and held in a glass capillary, allowing isotropic sub-micron-resolution imaging of the vasculature.
Together, these optical innovations enable us to cover a wide range of biological samples, including entire zebrafish vasculature and mouse organs such as the cochlea and brain vasculature.
We achieved isotropic sub-micron resolution with an axially sweeping light sheet running near the camera’s top frame rate. A high-frequency closed-loop system stabilizes the remote-focusing voice-coil motion, enabling 100 fps while preserving sub-micron resolution over a large FOV.
Unlike other light-sheet systems that use the remote-focusing arm only to sweep the sheet, we found it can also correct distortions such as field curvature. By using a concave instead of a flat mirror, we compensate curvature and expand the usable FOV across the full RI range.
Our microscope uses an air objective lens for illumination. To make the air objective suitable for creating a diffraction-limited light sheet in high-RI media, we use an off-the-shelf meniscus lens with a surface curvature matched to the NA of the illumination lens across all refractive indices.
Our compact, high-speed light-sheet fluorescence microscope is built from off-the-shelf optics to achieve 850 nm isotropic resolution for cleared samples up to 1 cm3, across refractive indices from 1.33 to 1.56.
Happy to see our paper “Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue” in @natbiotech.nature.com – with Tobias Moser’s lab from @unimedizin-goe.bsky.social and @janwenzel.bsky.social
www.nature.com/articles/s41...
#microscopy #lightsheet
Our image of a sea squirt oozoid (Thalia democratica) was selected as an Image of Distinction in Nikon Small World. Imaged with the Flamingo microscope by @metteh-thorsager.bsky.social at @biodev-vlfr.bsky.social in Villefranche-sur-Mer. Salp sample courtesy of Alexandre Alie.
nikonsmallworld.com/
Attend the talk “From ciliary beating to muscular locomotion: Developmental origins of nervous systems in the annelid Platynereis dumerilii” in the “neural circuit evolution” symposium presented by @metteh-thorsager.bsky.social
Visit poster #30 on “Pallium-encoded valence-specific chemosensory amplification of eye-body coordination in larval zebrafish” by @samuelsy20.bsky.social
Huisken lab is attending Göttingen Cognition Forum: Curiosity & Interaction. Learn about our research...
@spp2205.bsky.social @sfb1528.bsky.social @rtg2906-curiosity.bsky.social
@uni-goettingen.de events.gwdg.de/event/1099/
Shape the future of light: PhD opportunity in photonics in the Huisken lab. Apply through the Max Planck School of Photonics. Deadline 1 Dec 2025. Excellent research network. photonics.maxplanckschools.org/apply-now
Postdoc opportunity in the Averof lab: a crustacean meets a flamingo—not on the menu, but in the light sheet. Track the origins of cells during leg regeneration in Parhyale hawaiensis using light sheet microscopy. Excited to be part of this interdisciplinary project. Details on how to apply below.
Postdoc opportunity in the Averof lab: a crustacean meets a flamingo—not on the menu, but in the light sheet. Track the origins of cells during leg regeneration in Parhyale hawaiensis using light sheet microscopy. Excited to be part of this interdisciplinary project. Details on how to apply below.
Big thanks to the organisers @paveltomancak.bsky.social @bundschuhst.bsky.social @cuencam15.bsky.social @ereynaud.bsky.social @olafselchow.bsky.social!
The course showcased both commercial and home-built light sheet microscopes, plus hands-on introductions to image processing and analysis software. A fantastic two weeks surrounded by old colleagues and new collaborators.