Marie Winz

Postdoc in Structural Biology of RNA processing complexes Postdoctoral positions in structural biology of macromolecular complexes are available in the laboratory of Dr. Eva Kowalinski at the EMBL Grenoble, France. We are looking for highly motivated and amb...

Postdoc positions in structural biology of RNA processing complexes.

An ERC-funded position will dissect pre-mRNA processing pathways in the infective organism Trypanosoma brucei. An ANR-funded position will characterise large complexes in RNA modification.

Details: tinyurl.com/postdoc-RNA-...

Congrats!

Congrats!!!

Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has ...

🚨 New preprint! 🚨
We just dropped our latest work on RNA-binding proteins and sporulation in B. subtilis! Turns out, the RBP landscape gets a major remix during sporulation 👀. Check it out here 👉 www.biorxiv.org/content/10.1...
#RBP #RNA #sporulation #Bacillus #microbiology @lcbofficiel.bsky.social

UbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains Ubiquitin chains determine the fates of their modified proteins, including proteasomal degradation. Kiss et al. present UbiREAD, a technology to monitor cellular degradation and deubiquitination at high temporal resolution after intracellular delivery of ubiquitinated proteins. This reveals a degradation code for ubiquitin chains varying by linkage, length, and topology.

Online Now: UbiREAD deciphers proteasomal degradation code of homotypic and branched K48 and K63 ubiquitin chains Online now:

4. Backtracked RNA can be polyadenylated (AAA) on the 3' end by the TRAMP complex. We speculate that AAA-marked nascent RNA could either be degraded or trigger reverse torpedo termination.

Although this work focuses on RNAPI, many findings apply broadly to RNAPs

3. We proposed an alternative model for torpedo termination, where a 5'→3' exonuclease trims the nascent RNA to RNAP boundaries, followed by backtracking-mediated cleavage.

2. We uncovered multiple mechanisms contributing to RNAPI transcription termination, including road-block termination, end-nucleolytic cleavage, and Rat1 torpedo termination.

Summary by Tomasz:
Some key takeaways:
1. The first experimental evidence supporting the model of immobilized RNAPs and spinning DNA—validated by experimental confirmation of theoretical predictions.

Paper alert 🚨 Check out our new paper about RNA polymerase I termination! Great collaboration with
Liz Petfalski, Katarzyna Grelewska-Nowotko and David Tollervey!
online yesterday in @CellReports! It took a while but finally come to life! t.co/5RPpxAhRvU
It was a pleasure to contribute!