Many thanks for sharing, Paulo! ;)
Thank you!!!
Thank you for the compliment. Full transparency: the cartoon was AI-assisted — but it required serious scientific mentoring 😂 Convincing ChatGPT that anaphase = sister chromatids, not X-shaped chromosomes was a multi-cycle process. Peer review works, even for AI!
Thank you for the compliment. Full transparency: the cartoon was AI-assisted — but it required serious scientific mentoring 😂 Convincing ChatGPT that anaphase = sister chromatids, not X-shaped chromosomes was a multi-cycle process. Peer review works, even for AI!
Congratulations, @ltovini.bsky.social! What a journey this has been! This story would not exist without your determination, resilience, and scientific imagination. You kept pushing when things were difficult and turned complexity into clarity. I am super proud of you!!
Thanks for the nice words! Indeed... yeast could be seen as an exception (as they keep transcription mostly on) but not all over :D also needs to be off at some sites. And there are even more interesting things on Pol I that will come on another upcoming ms. Stay tuned ;)
5/ This means that mitotic transcription isn’t just a passive consequence of cell cycle stage — it actively threatens genome stability when not properly regulated.
4/ The result? When transcription isn’t cleared, sister chromatid resolution is impaired — a critical step needed to ensure faithful chromosome segregation in anaphase.
3/ Importantly, we show that these defects arise because R-loops form when transcription persists during mitosis — and inhibiting transcription reverts the defects. That establishes a causal link.
2/ Using TTF2 depletion to force nascent transcripts to persist on mitotic chromatin, we found that this abnormal retention leads to mitotic errors including abnormal chromosomes and bridges.
1/ During mitosis, cells globally suppress transcription as chromosomes condense — a conserved hallmark of cell division. But what if this repression fails? 😮💨
🧵 Thread: What happens when transcription isn’t properly shut off during mitosis? Our new preprint explores just that 👇
www.biorxiv.org/content/10.6...
4/ The result? When transcription isn’t cleared, sister chromatid resolution is impaired — a critical step needed to ensure faithful chromosome segregation in anaphase.
3/ Importantly, we show that these defects arise because R-loops form when transcription persists during mitosis — and inhibiting transcription reverts the defects. That establishes a causal link.
2/ Using TTF2 depletion to force nascent transcripts to persist on mitotic chromatin, we found that this abnormal retention leads to mitotic errors, including abnormal chromosomes and bridges.
1/ During mitosis, cells globally suppress transcription as chromosomes condense — a conserved hallmark of cell division. But what if this repression fails? 😮💨
It was an honour to present at such a special meeting and get to know other recently elected EMBO members (and “older” too). Many thanks to Helder Maiato for the nomination and to all members and staff for the warm welcome.
Slightly outdated (September issue) but I think it is still worth the honour of 1st post on BlueSky :) check the highlighted manuscript here: www.embopress.org/doi/full/10....
It was a pleasure to meet you!! Fantastic meeting indeed! Thanks for bringing me here :)
