Exosome tethering requires tetherin homodimerisation www.biorxiv.org/content/10.1...
08.08.2025 15:32 β π 1 π 1 π¬ 0 π 0@holtra-tech.bsky.social
Technology for the future of nanoparticle analysis https://www.holtra.tech Based in Gothenburg, Sweden Here for all things nano, extracellular vesicle, #nanomedicine, #virus, #exosome, #EV, #LNP
Exosome tethering requires tetherin homodimerisation www.biorxiv.org/content/10.1...
08.08.2025 15:32 β π 1 π 1 π¬ 0 π 0In summary, holographic microscopy offers label free, single shot, high resolution, high frame rate, 3D imaging! It sounds almost too good to be true, and that is why we are so convinced of itβs potential and excited to be developing microscopes around this technology!
12.08.2025 09:00 β π 0 π 0 π¬ 0 π 0For holographic microscopy, instead of physically illuminating the interference patterns to reveal the hologram, the hologram is reconstructed computationally. With rapid advances in processing power for these kinds of tasks, the potential of holographic microscopy is only growing!
12.08.2025 08:53 β π 0 π 0 π¬ 1 π 0This means that from a single light exposure a hologram captures a 3D representation of the object! The images of the mouse below are produced by viewing the same hologram from different angles!
12.08.2025 08:53 β π 0 π 0 π¬ 1 π 0To create a hologram, the light coming from the object is merged with another beam, creating interference pattern between the two beams. When the object is removed and the interference pattern illuminated, light is diffracted and a 3D image of the original object appears.
12.08.2025 08:53 β π 0 π 0 π¬ 1 π 0When light from an illuminated object is focussed and captured by a camera a 2D image is recorded. A hologram however is a 3D representation of the object of interest and is recorded differently. Relying on interference patterns similar to those seen between waves on water.
12.08.2025 08:53 β π 0 π 0 π¬ 1 π 0Firstly, what is a hologram?
holograms, like the Visa dove below, are very familiar. but what are they? Much like a recording of a sound, e.g. music, a hologram is a 3D βrecordingβ of an illuminated object that can be replayed, even once the object is removed. But how are they created?
What is holographic microscopy?
And why are we so excited about itβs scientific potential?
Size is a key descriptor of extracellular vesicles #EV. In samples containing contaminating particles with an overlapping size distribution, size alone is insufficient. Measuring refractive index at the individual particle level enables differentiation of extracellular vesicles and lipoproteins.
11.08.2025 12:29 β π 0 π 0 π¬ 0 π 0For this study DAISY was used to monitor the purity and consistency of cell culture-derived exosome preparations. Check out our application not on DAISY for extracellular vesicles to learn more.
www.holtra.tech/en/applicati...
Excited to share this pre-print from our collaborators, the Edgar lab, at the University of Cambridge, UK
www.linkedin.com/posts/james-...
Get in touch with us if you want to see for yourself how DAISY can revolutionise your nanoparticle analysis!
www.holtra.tech
To measure phase shift, DAISY employs digital holographic microscopy (DHM). DHM separates phase and intensity information to generate a 3D image of the light scattered by each particle from a single exposure.
08.08.2025 08:23 β π 2 π 0 π¬ 1 π 0Alongside size, DAISY measures the refractive index of each particle. It does this by directly measuring the phase shift of light passing through the particles. Denser materials give higher phase shift which means that, uniquely, DAISY can differentiate particles based on their composition!
08.08.2025 08:23 β π 0 π 0 π¬ 1 π 0Nanoparticle tracking analysis (NTA) records particles diffusing in solution. The smaller the particle the faster the diffusion. This is how NTA assigns a size.
08.08.2025 08:23 β π 0 π 0 π¬ 1 π 0What is DAISY?
DAISY is next generation nanoparticle tracking analysis!
We will also be presenting at the European National Societies of Extracellular Vesicles MOVE symposium in Tartu, Estonia Oct 7th to 9th:
move2025.ee
See you there!
It is shaping up be a busy autumn! HOLTRA are excited to be presenting our technology at the European Colloid and Interface Society meeting in Bristol, UK. Sept. 7th to 12th
www.ecis-web.eu/about-us/eci...
DAISY can see inside your liposomes and lipid nanoparticle preparations to reveal internal structure!
Read more here:
www.holtra.tech/en/applicati...
DAISY nanoparticle tracking in action!
sample is 415 nm silica beads
Do you want to easily identify and quantify contaminants and aggregates in your extracellular vesicle samples?
Take a look at our EV application note to find out how DAISY can improve your EV analysis:
www.holtra.tech/en/applicati...
Check out our updated website for an overview of our technology and real-world applications in nanoparticle analysis:
www.holtra.tech