Santosh Phuyal

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

The endoplasmic reticulum proteostasis network and bone disease Bone homeostasis relies on the coordinated activity of osteoblasts and osteoclasts that balances bone formation and resorption, and of osteocytes for biomechanical sensing and hormone secretion. A key...

Our review on the role of ER proteostasis in bone disease is out today in Trends in Molecular Medicine www.cell.com/trends/molec...

And the current publishing akd reviewing system

Colorful mural painted on the side of a building with the likeness of Henrietta Lacks and the words “HeLa” and “Middle East” on it.

Beautiful mural honoring Henrietta Lacks painted by Baltimore community members popped up near Hopkins med campus recently.

Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩

Interested in lysosome damage and repair pathways? Check out the latest updates.

Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor ER-membrane contact sites are often distorted by existing labeling methods. This study develops an inducible labeling system called LaBeRing that uses the Lamin B receptor to fluorescently tag a varie...

Check out our lab's latest paper. Laura Downie found that the Lamin B Receptor (LBR) can be used to inducibly label all kinds of membrane contact sites in live cells
doi.org/10.1371/jour...

Targeting proteostasis in multiple myeloma through inhibition of LTK Leukemia - Targeting proteostasis in multiple myeloma through inhibition of LTK

Our new paper is out in Leukemia on Targeting proteostasis in multiple myeloma through inhibition of LTK. Thanks to Ludvig and Thea for this great collaborative work
rdcu.be/evyfX

The system is versatile enough to allow for measuring forces as a result of cell-cell interactions? Not just at the PM but deep within the cells.

Genetically encoded mechano-sensors with versatile readouts and compact size Mechanical forces are critical for virtually all fundamental biological processes, yet quantification of mechanical forces at the molecular scale in vivo remains challenging. Here, we present a new st...

Molecular force measurements in vivo are notoriously difficult. We present a new class of programmable force sensors based on engineered coiled-coils, with modular readouts, including fluorescence and luminescence. doi.org/10.1101/2025...

It is that time of the year 🎄🎅

Just finished reading The Mercy of Gods, and what a great book it is! 📚 Can't wait for the other books in the series

Efficient non-viral immune cell engineering using circular single-stranded DNA-mediated genomic integration - Nature Biotechnology CAR-T and NK cells engineered using a non-viral circular single-stranded DNA system show effective anti-tumor function.

This is pretty wild! Efficient non-viral genome editing of immune cells using circular single-stranded DNA. #science #hemesky 🩸🔬 www.nature.com/articles/s41...

Cool movie! Now that i also fall under "accidental immunologists" category i can fully appreciate the amount of work that went in this video :-) Which CRISPR protocol did you follow for the KI?

Today, I discovered that google's NotebookLM generates quite nice podcasts based on pdf files of scientific research articles with great accuracy. Pretty neat #AI tool.

PIEZO-dependent mechanosensing is essential for intestinal stem cell fate decision and maintenance Stem cells perceive and respond to biochemical and physical signals to maintain homeostasis. Yet, it remains unclear how stem cells sense mechanical signals from their niche in vivo. In this work, we ...

Perfect reason for my first post here. Super excited to see this work on PIEZO mechanosensing of the intestinal stem cell niche led by Meryem Baghdadi @vignjeviclab.bsky.social and Kim lab out in Science! Honored to have contributed to this story as 2nd author. 🎉

www.science.org/doi/10.1126/...

Mechano-dependent sorbitol accumulation supports biomolecular condensate Torrino et al. identify the mechanosensitive polyol pathway as a natural mediator of cytoplasmic and nuclear protein condensates through tightly controlled intracellular sorbitol accumulation. Extrace...

Mechano-dependent sorbitol accumulation supports biomolecular condensate
🧪
www.cell.com/cell/abstrac...

Cancer-associated fibroblasts maintain critical pancreatic cancer cell lipid homeostasis in the tumor microenvironment Han et al. identify crosstalk between CAFs and pancreatic cancer cells under hypoxia and nutrient scarcity. Lysophosphatidylcholines (LPCs), as the key mediators, can be utilized for phosphatidylcholi...

Cancer-associated fibroblasts maintain critical pancreatic cancer cell lipid homeostasis in the tumor microenvironment www.cell.com/cell-reports...

🎨 In case anyone need…

The NIH BioArt Source is an awesome library of *free* professionally drawn illustrations for scientific presentations or figures. Downloadable in HD. Thank you NIH for this invaluable tool 🙏!

Check it out 👇
bioart.niaid.nih.gov

Beautiful paper from Burkhard Ludewig’s group showing the importance of immunoregulatory stroma to support anti-tumour immunity.

www.cell.com/action/showP...

Here are some free/open-source sites for the preparation of scientific illustrations, including icon libraries: bioicons.com, scidraw.io, bioart.niaid.nih.gov, inkscape.org, biogdp.com, reactome.org/icon-lib

Totally. It is also painful process when reviewers comment "unconvincing data or data doesn't support conclusion", without offering ways to address raised points