Rhett Rautsaw

SPRQ-Nx is coming to #Revio next year. It is expected to reduce whole genome sequencing costs by up to 40% to $300–$350 per genome or lower at scale.

#PacBio #ASHG25 #HiFisequencing #ASHG

The #PacBioBlockParty is in full swing 🎉 Guests are rolling in, the music’s on, and the fun’s just getting started! Virtual darts, bowling, food, a live DJ, and more await. Let’s celebrate together!
 
#ASHG25 #PacBio #ASHG

PacBio: $300 Genome Via Chemistry Update 🧬🖥️

omicsomics.blogspot.com/2025/10/pacb...

Short reads…who needs em? Start bringing ALL your NGS projects into the long read era with cheaper sequencing, simultaneous methylation, greater insights, and more answers to your biological questions. PacBio’s got you covered.

Just in: #PacBio HiFi long-read sequencing is powering the National Institute on Aging’s Long Life Family Study.

Up to 7,800 genomes and epigenomes will be sequenced on Revio systems to uncover rare variants, methylation patterns, and other factors linked to healthy aging.

🔗 bit.ly/3IUDdFo

I'm heading to Boston for #ASHG25 tomorrow, let me know if you'd like to chat about using HiFi sequencing for mitochondrial analyses, pharmacogenomics, or anything else!

ICYMI, also happy to discuss our new Aardvark tool for benchmarking :)

Release StarPhase v1.5.0 · PacificBiosciences/pb-StarPhase Changes Adds a new field to the output json, inexact_diplotypes. This field is intended to capture information about inexact allele matches. The behavior of this output depends on the gene: For s...

We just released a major update for StarPhase that adds support for inexact matching PGx alleles. We're hoping this streamlines the process for discovering novel allele combinations with #PacBio #HiFi data, feedback welcome!

Full notes here: github.com/PacificBiosc...

#ASHG25

a little boy is driving a red toy car on the street . Alt: a little kid is driving a red toy car on the street.

Updated tutorials for nQuack today. Want to predict ploidy level of sequence data? See this new tutorial on how to process and model data faster: mlgaynor.com/nQuack/artic...

Population-scale Long-read Sequencing in the All of Us Research Program The All of Us Research Program (AoU) is a national biobank seeking to enroll one million individuals in the United States to link genomic and biomedical data, including short- and long-read whole-geno...

In a landmark #PopulationGenomics study, All of Us researchers used #HiFisequencing of 1,027 participants to identify hundreds of #StructuralVariants disease associations, with over half missed by short reads. What are you missing in your research?

Read the study here: bit.ly/4ocq49K

#PacBio

Now published in Cell! We found that ~15% of SNPs from divergent refs did not liftover as SNPs in the gray fox ref—half mapped to monomorphic sites, half failed to map. Co-authored with Matthew Genchev, @elliecat.bsky.social, and @jazlynmooney.bsky.social

www.sciencedirect.com/science/arti...

It absolutely warms my heart when I click on a pre-print that includes a bunch of top methods folks from industry and I see (1) open-source software (with a good license; MIT in this case) that is (2) written in Rust. Bravo @pacbio.bsky.social!

Moar Rust plz!

github.com/PacificBiosc...

I'm excited to share our pre-print about a new variant benchmarking tool we've been working on for the past few months!

Aardvark: Sifting through differences in a mound of variants
GitHub: github.com/PacificBiosc...

Some highlights in this thread:
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The 2025 #Epigenetics SMRT Grant is open!

Submit a 100-word proposal for a chance to win free sequencing with Fiber-seq, which combines genome sequence, 5mC methylation, and chromatin accessibility for a multiomic view of regulation.

Apply here: bit.ly/4nGQrEW

Today marks the first day in public media’s history without federal funding. And we’re not going anywhere.

Listeners like you keep our mission alive. Protect one of the last places where America comes together to hear itself.

Stand with us today. Donate at this link: n.pr/46wamAj

Did you know? Revio & Vega include on-instrument 6mA calling for the Fiber-seq chromatin accessibility assay. Thanks to Fiber-seq expert @mrvollger.bsky.social for the thoughtful evaluation and recommendation.

Reagents for the assay are also available from our friends at EpiCypher: bit.ly/42YwGQE

Can one test replace many?

A JAMA Pediatrics study by @childrensmercy.org evaluated long-read HiFi sequencing as a first-line approach for pediatric rare disease research, with potential to improve solves, reduce turnaround time, and bypass multiple sequential testing.

Read more: bit.ly/3VtHjHh

🚨 Last call! This week, join PAG’s free webinar to see how long-read low-pass sequencing and pangenomes are reshaping #genomics.

From structural variation to trait discovery, explore scalable insights across species. Sponsored by #PacBio.

Register: bit.ly/4mbCkFU

The 2025 Long-Read Low Pass SMRT Grant for plant + animal sciences is now open! 🌱🐾

Apply to win free PacBio HiFi sequencing for your conservation or agricultural research.

Submit your 100-word proposal today: bit.ly/3IgrReu

#PacBio #SMRTGrant #HiFisequencing

Ready to celebrate #ASHG25? 🎉

Join us at the PacBio Block Party on October 15 in Boston to network with fellow scientists, connect with the genomics community, and honor 25 years of discoveries and innovation.

RSVP now: bit.ly/46xnUeI

#PacBioBlockParty

Volume 79, Issue 8 August 2025 Cover image

New Editor's Choice article:
"Island biogeography and competition drive rapid venom complexity evolution across rattlesnakes" by Hirst et al.
doi.org/10.1093/evol...
@hirsticane.bsky.social @RamsesRosales11.bsky.social @marcabeer.bsky.social @jason-strickland.bsky.social @reptilerhett.bsky.social

Lego versions of PacBio Sequel IIe, Revio, Onso, and Vega instruments

Lego version of the Vega

Vega has joined the @pacbio.bsky.social “lego” family. #HiFi #DNA #LongReads

From low-pass seq that scales discovery to pangenomes that reveal hidden variation, long-read technologies are transforming genomics.

Join PAG’s free webinar to explore insights reshaping research across the tree of life. Sponsored by #PacBio

Register: bit.ly/4mbCkFU

Salary to Living Wage - only two points fall above the 1:1 line. A star indicates New Jersey Institute of Technology is almost to the 1:1 line!

Always excited for an increased salary submission! Congrats to the Department of Biological Sciences at New Jersey Institute of Tech for their stipend increase! This union-based win brings the pay almost to the 1:1 line! Update your department and find out more here: rhettrautsaw.app/shiny/Biolog...

Most RNA-seq methods feel like stir-fry. Transcripts diced into bits, then tossed back together.

HiFi long reads bring the whole roast. Full transcripts served intact, end to end.

Need to see how? Get the Seq 101 guide & a chance at a sponsored conference: bit.ly/3JminPa

#TheresHiFiForThat

Looking to adopt long-read sequencing in your lab?

The #PacBio Pre-Clinical Playbook has best practices, workflow guidance, case studies, and real-world insights to help you implement #HiFisequencing with confidence.

🔗 Download now: bit.ly/4mpZ85b

Get the most out of your HiFi data with @pacbio.bsky.social tools! Available for free on GitHub and bioconda/containers

Unlock the power of #PacBio HiFi whole genome sequencing in our 4-part video series.

Learn from PacBio scientists as they walk through tools and examples for variant calling, tandem repeat analysis, paralogous gene resolution, and star (*)-allele calling.

Sign up: bit.ly/3HV8Fmh

Long-Read Low-Pass Sequencing for High-Resolution Trait Mapping Accelerating crop improvement is critical to meeting food security demands in a changing climate. Long-read sequencing offers advantages over short-reads in resolving structural variations (SVs) and a...

HiFi is getting cheaper. You should talk to the group at Veil Genomics (www.veilgenomics.com)

www.biorxiv.org/content/10.1...

Curious how long-read sequencing is shaping drug discovery and development? Hear it from the experts.

Join panelists from Pfizer, AstraZeneca, GSK, and more as they share how #PacBio HiFi sequencing is transforming the #biopharma industry.

Register now: bit.ly/4m3L7KJ

Hi #Botany2025 - In my quest to never do flow cytometry again, I am collecting sequence data (radseq, target enrichment, WGS, ect.) with confirmed ploidal level. If you have samples, reach out!