Ina Klockner 🧬👩‍🔬🔬

We’re excited to announce the launch of our new website at angelman.org! 🎉 Our updated site features easy navigation and filters that help you find the resources, clinics, research, and support you need on any device. Check it out!➡️ angelman.org

A Boa Constrictor brain-derived cell expressing a viral glycoprotein (magenta) and stained for actin (yellow) and DNA (white).

#FluorescenceFriday

Added!

Sure! I will add you ASAP 🙂

FINALLY out in #Science Advances! 🎉
3 years of hard work, a fantastic team — and I’m proud to be first and co-corresponding author!

It's about Plexin signaling driving collective cell migration & organ sculpting. Check out the videos 😀

www.science.org/doi/10.1126/...

#DevBio #CellBio #Microscopy

For #fluorescencefriday the Mesoscopic Integrated NeuroImaging Data (MIND) platform @westernu.bsky.social presents abeta plaques and blood vessels in the 🧠 of our ApoE4 mouse model of AD. 🧪 #clearing #lightsheet 🔬 Courtesy of Arash Salahinejad, Luke Smolders and @shahrzadbahrampour.bsky.social

Stunning images!

1)
Branching morphogenesis is fundamental to animal development, shaping complex organs like lungs, kidneys, and vascular networks. Gills are another highly branched organ. Yet, the developmental processes behind gill formation in zebrafish remain poorly understood.

#zebrafish #gills #microscopy

Gills just wanna have #FluorescenceFriday 🎵

Phalloidin reveals the beauty of the filament's distal tip in cleared gills of adult 🦓🐟

#microscopy #devbio #sciart

I know, it's a bit late for #FluorescenceFriday but I want to share my new banner :D

#cellmigration #cellbio #devbio #sciart #microcosm #science

I begrudgingly admit that actin is pretty cool 🤩

🎥 Primary mouse myotubes labeled with Lifeact-mScarlet-I3 (cyan) and H2B-mStayGreen (red). #FluorescenceFriday

2/4 I chose this picture because I am interested in an old question:

How do fly testes obtain their specific shape?

In my previous research I learned that muscle precursors shape it, using collective cell migration and alignement to achieve the pattern visible in this picture.

Stand up for science Raleigh!! Let’s keep this energy going!!

Mesonephric glomeruli of #zebrafish for #FluorescenceFriday 🔬
kdrl-mCherry nicely reveals the capillary tuft of the glomerulus.

The zebrafish kidney stops developing at the mesonephros stage! It serves as their main kidney structure, and they don't develop a metanephros like humans do.

For today's #fluorescencefriday I have a riddle 😊. Can anyone who is knowledgeable in #Drosophila #neuroscience tell my what these are? I honestly don't know! Overview picture in the comments-they are from the brain🧠🪰. The marker is lifeact::GFP, depth-coded...
#sciart #microscopy #cellbio #cellfy🔬

An ancient unpublished picture from my PhD
#FluorescenceFriday!

Drosophila sperm pump depth-coded
with muscles (phalloidin=cyan/green) + Dapi (yellow/red).

Not confocal but an old Zeiss Apotome - which is still an awesome scope! #sciart #microscopy #bioart #sciart #microscopyart #cellbio #devbio

Wow! We got the cover! #MBOC #ASCB #CellBio
So proud of #TeamTestis. Screening <270 lines for shape defects was a huge team effort!

I created this collage from the team’s images from the past 2 years! Depth-color-coded with Fiji. Inspired by late 19th century lithographic animal illustrations.

🔬My first try on #FluorescenceFriday
Nice detail of a tissue-cleared kidney from neonatal mice.
-GFP is expressed in podocytes and pericytes.
-CD31 antibody highlights the branched blood vessels and glomeruli in red

How proximal is proximity labeling❓ Using DNA nanorulers we found that TurboID/BioID are largely contact labeling, while APEX2 also bears a major contact labeling pathway in parallel with diffusive labeling.
www.biorxiv.org/content/10.1...

Special thanks to @aliceyting.bsky.social for discussions

Muscles growth by sarcomere divisions - how your muscles add new sarcomeres during developmental growth: they divide! Cool collaboration @friedrich-group.bsky.social @raunser-lab.bsky.social @Dirk Görlich @univ-amu.fr @cnrs.bsky.social sciencecast.org/casts/aspy9l... www.biorxiv.org/content/10.1...

For those interested in "Genome editing and CRISPR technologies":

(if you are new here at Bluesky), here are the two starter packs...

bsky.app/starter-pack...

bsky.app/starter-pack...

The title of my talk is Plexin/Semaphorn Signaling Orchestrates Collective Cell Migration. It will be on 12/16 at 4PM in Room 33B!

Excited to give my 1st talk on an #ASCB meeting about collective #cellmigration in #Drosophila! #cellbio2024
12/16 4pm Room 33B, more info below👇!
Next, I'll post about our 2 posters!🪰

Thank you @allysonsgro.bsky.social & Daniel Cohen for this opportunity!
#cellbio #devbio @ascbiology.bsky.social

1/2 #TeamTestis postbac Sarah Clark presents a really cool poster: A large reverse-genetic screen identifies numerous regulators of testis nascent myotube collective #cellmigration and collective organ sculpting Tuesday, Dec 17 1:30 PM P2783, B400

#cellbio #devbio #cellbio2024 #Drosophila

Next to my talk (12/16 4 pm room 33B), I will present a poster: "Semaphorin/Plexin Antagonism Orchestrates Collective Cell Migration in vivo". Tuesday, Dec 17 11:15 AM.

P2780/Board No. B397

I am so excited. I just love ASCB meetings!

#cellbio2024 #cellbio #devcell @ascbiology.bsky.social #ASCB

Super recent publication:
AAV vector-derived elements integrate into Cas9-generated double-strand breaks and disrupt gene transcription
#AngelmanSyndrome

doi.org/10.1016/j.ym...

I just added you! Please share with others :)

Added you 😃

Just added you! Please let others know & share it :)

Super proud and honored to give my first #GRC talk at the Directed Cell Migration #GordonConference in Italy January 12-17 www.grc.org/directed-cel...

Thank you so much Sally Horne Badovinac and @robinsall.bsky.social for giving me this fantastic opportunity!

#cellbio #devbio #cellmigration