Luciano A. Masullo

Thanks!!

Juliii ❤️ thanks for your kind words!! Looking forward to be part of the wonderful optics community in Argenitna!!

The image on the cover shows two sugars from the same cell-surface glycan separated by 9 Å, visualized with RESI (resolution enhancement by sequential imaging) enabled by metabolic labelling with DNA barcodes. IMAGE: Luciano A. Masullo, Max Planck Institute of Biochemistry, Germany. COVER DESIGN: Vanitha Selvarajan Original paper: Masullo, L.A., et al. Ångström-resolution imaging of cell-surface glycans. Nat. Nanotechnol. 20, 1457–1463 (2025). https://doi.org/10.1038/s41565-025-01966-5 Abstract: Glycobiology is rooted in the study of monosaccharides, ångström-sized molecules that are the building blocks of glycosylation. Glycosylated biomolecules form the glycocalyx, a dense coat encasing every human cell with central relevance—among others—in immunology, oncology and virology. To understand glycosylation function, visualizing its molecular structure is fundamental. However, the ability to visualize the molecular architecture of the glycocalyx has remained challenging. Techniques such as mass spectrometry, electron microscopy and fluorescence microscopy lack the necessary cellular context, specificity and resolution. Here we combine resolution enhancement by sequential imaging with metabolic labelling, enabling the visualization of individual sugars within glycans on the cell surface, thus obtaining images of the glycocalyx with a spatial resolution down to 9 Å in an optical microscope.

Now online: October 2025 Issue.

- Focus Issue on #biosensing,
- DNA moiré superlattices,
- Sugars at Ångström-resolution,
- Solid-state #nanopores,
- Non-aqueous Li #batteries, -
- Neuromorphic vision,
- Peptide #hydrogels,
- Deep learning for #LNPs and more...

www.nature.com/nnano/volume...

Thread describing our work: bsky.app/profile/luma...

Our article "Ångström-resolution imaging of cell-surface glycans" made it to the cover of Nature Nanotechnology! 🤩 @natnano.nature.com

www.nature.com/nnano/volume...

Thank you, Inês! 😀

I am also very grateful to my previous mentors, Ralf Jungmann and Fernando D. Stefani, and many other amazing colleagues who have supported me throughout my career.

I am very grateful to Gabriel Rabinovich for his generous sponsorship and guidance throughout the application process, and I look forward to developing my research program within the stimulating environment of the Glycosciences Program at IBYME.

I’ll soon be looking for Master’s students, PhD students, and a postdoc to join the team—so if this sounds exciting to you, please don’t hesitate to reach out!

My research will focus on deciphering cell signaling at the molecular level by applying and developing cutting-edge super-resolution methods, with a particular emphasis on glycoimmune checkpoints, aiming to translate this knowledge into novel and improved therapies.

This grant gives me the opportunity to start building my own group, with support for setting up a state-of-the-art super-resolution microscopy lab.

Thrilled to announce some big news!

Excited to share that I’ve been awarded a Wellcome Trust Early Career Award to establish my research as a Group Leader in the Glycosciences Program at the Institute of Biology and Experimental Medicine (IBYME) in Buenos Aires, Argentina, beginning in early 2026.

Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)

To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)

We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)

The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)

Highly efficient 12-color multiplexing with speed-optimized DNA-PAINT. We are excited to share our latest paper in @natcomms.nature.com, using left-handed DNA to extend speed-optimized DNA-PAINT to 12 targets in a simple and straightforward way! 🧬👈🚀https://www.nature.com/articles/s41467-025-64228-x

One talk by Luciano Masullo at 10 am and another talk at 3 pm by Adam Cohen, both next Tuesday, October 7, at the MPI for Medical Research Heidelberg.

📣 📅 Invitation to two talks at our institute next Tuesday, October 7: 🤩 Luciano Masullo @lumasullo.bsky.social from @mpibiochem.bsky.social at 10 am, Adam Cohen @harvard.edu at 3pm, also online:
us06web.zoom.us/webinar/regi...

#maxplanck #mpimr #colloquium #medicalresearch #sciencetalks #science

Localization of single molecules with structured illumination and structured detection - Light: Science & Applications Light: Science & Applications - Localization of single molecules with structured illumination and structured detection

Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field!

www.nature.com/articles/s41...

Congratulations, Rita! And thanks for all the amazing work at Nature Methods!

Thanks so much, Mahipal!!

Ångström-resolution imaging of cell-surface glycans

Now published in Nature Nanotechnology! 🤩
@natnano.nature.com

www.nature.com/articles/s41...

Ångström-resolution imaging of cell-surface glycans - Nature Nanotechnology By combining bioorthogonal metabolic labelling and resolution enhancement through sequential imaging of DNA barcodes, the molecular organization of individual sugars in the native glycocalyx has been ...

New paper online:

Ångström-resolution imaging of cell-surface glycans.

The molecular organization of sugars in the native #glycocalyx has been resolved at 9 ångström using bioorthogonal metabolic labeling and #superresolution imaging of DNA barcodes.

#Glycotime

www.nature.com/articles/s41...

Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI - Nature Communications The nanoscale organization of the antigen-antibody complexes influences the therapeutic action of monoclonal antibodies. Here, the authors present a multi-target 3D RESI imaging assay for the nanomete...

Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6)
Read the full story here: www.nature.com/articles/s41...

Super-resolution imaging in whole cells and tissues via DNA-PAINT on a spinning disk confocal with optical photon reassignment - Nature Communications Zaza and colleagues demonstrate that DNA-PAINT on a spinning disk confocal microscope with optical photon reassignment enables high-resolution imaging across large fields and imaging depths, resolving...

Can we break past the usual limits of #SuperResolution #Microscopy—achieving deep-imaging, high-res, large FOV imaging without the trade-offs?
🧬🔬 YES: with #DNA_PAINT + Spinning Disk Confocal + Optical Photon Reassignment (#SDC-OPR)!
📄 www.nature.com/articles/s41... @natcomms.nature.com!
🧵👇

Congrats!! It’s an amazing story! 🤩

Big congrats, Florian!! 🥳🤩 All the best at this new super exciting position!! 🚀

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

A new study by @jungmannlab.bsky.social presents #SPINNA, a model-based tool for quantifying protein stoichiometry, oligomerization state, and spatial arrangement from super-resolution data.🔬For precise labeling of #GFP & #mCherry fusion proteins researchers used our #nanobodies 😊
📄 bit.ly/4jTHfes

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

We are excited to present our latest work published in @natcomms.nature.com

www.nature.com/articles/s41...