Jesse Garcia Castillo, PhD

Lipid metabolism drives dietary effects on T cell ferroptosis and immunity @nature.com www.nature.com/articles/s41...

A glucocorticoid–FAS axis controls immune evasion during metastatic seeding @nature.com @danafarber.bsky.social @harvardmed.bsky.social @judithagudo.bsky.social
www.nature.com/articles/d41...
www.nature.com/articles/s41...

New research out in Nature Immunology finds that tissue-resident exhausted & memory CD8+ T cells have distinct ontogeny, function & role in disease ft. Simone Park & @ejohnwherry.bsky.social (Systems Pharmacology & Translational Therapeutics/ @i3h-upenn.bsky.social) www.nature.com/articles/s41...

@simoncleary.bsky.social, Longhui Qiu, Mark Looney et al. developed a new #intravital technique for imaging pulmonary lymphatics. rupress.org/jem/article/...

📘 In our #Lymphatics collection: rupress.org/jem/collecti...

Happy to share our new paper in @febsj.bsky.social! With
@ondrej-kuda.bsky.social & @fedorovalab.bsky.social we show how #LipidDroplets reshape the lipidome of PUFA-exposed cells, but can also shift function within the same cell, revealing a context-dependent role in #ferroptosis. shorturl.at/TyRl1

Finally someone did a nice characterization of TSC22D3 (GILZ) in T cell activation I know you T cell omics people have been wondering

The main function of the TCA cycle is to feed electrons into the respiratory chain.
..wait, what!? It also clears excess citrate, because otherwise you get severe kidney damage?
Great example that textbooks aren’t always the full story by Abby, @juliabrunner.bsky.social and @lydiafinley.bsky.social

Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle Beyond its traditional role in supporting bioenergetic and biosynthetic demands, the canonical TCA cycle is also essential for nutrient clearance. When pyruvate oxidation and citrate production increase, aconitase 2 becomes essential to prevent mitochondrial citrate accumulation, which can trigger the integrated stress response and impair cell fitness.

Now online! Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

Vy2+ γδT17 development is perturbed in the neonatal period. (A) Bulk, Vγ2+, and Vγ4+ thymus cellularity in day 1–2 (D1–2) WT and RasGRP1 KO mice. (B) Proportion of γδ thymocytes in CD24/CD73 quadrants in D1–2 WT and RasGRP1 KO mice. (C) Proportion of γδ thymocytes producing IL-17 in D1–2 WT and RasGRP1 KO mice after 3 h PMA/IM stimulation. (D and E) Proportion of (D) IL-17+ γδ thymocytes that are Vγ2+, Vγ4+, or Vγ1.1+, or (E) percentage of Vγ2+, Vγ4+, or Vγ1.1+ γδ thymocytes that are IL-17+ in D1-2 WT and RasGRP1 KO mice after 3 h PMA/IM stimulation. Data are pooled from 2 independent experiments of 2–7 mice per experiment. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Researchers defined the role of RasGRP1 in the development and effector programming of γδ T cells. RasGRP1 activation serves as an important signaling hub, which integrates signals from both non-TCR and TCR inputs to direct differentiation. Read more: https://ow.ly/rXll50Yn51v.

Effector differentiation by stem-like intraepithelial γδ T cells is required for host defense against infection
www.cell.com/immunity/ful... @cp-immunity.bsky.social

A PI3Kd-Foxo1-FasL signaling amplification loop rewires CD4+ T cell signaling and differentiation. New study from Dominic P. Golec, Pamela L. Schwartzberg and colleagues @niaidnews.bsky.social: rupress.org/jem/article/...

#Immunodeficiency #LymphocyteBiology

TOX has opposing functions in CD4 & CD8 T cells, driving exhaustion in the latter but promoting Th1 commitment in CD4 T cells that drive antitumor immunity and pathogenic autoimmune responses
www.nature.com/articles/s41...
@natimmunol.nature.com @mskcancercenter.bsky.social

CD38 expression by neonatal human naïve CD4+ T cells shapes their distinct metabolic and tolerogenic properties @jci.org @ucsfmedicine.bsky.social
www.jci.org/articles/vie...

Metabolic quiescence of naive-like memory T cells precedes and maintains antigen-specific T cell memory - Nature Immunology Schober and colleagues show that effector CD8+ T cells undergo metabolic shutdown, CD8+ central memory T cells are the most metabolically active, and naive-like memory T cells are quiescent during the...

New work from our lab, hot off the press in Nature Immunology (@natureportfolio.nature.com)!

How do human T cell responses diversify — and how does this lead to durable protection after vaccination?

Paper: www.nature.com/articles/s41...

Press release: www.fau.eu/2026/02/news...

1/6

New feature to help plan Modular Cloning (Golden Gate) experiments and streamline the otherwise confusing dance of overhang compatibility and vector hopping. Gonna find some time to try this out and compare to my current SnapGene workflow. Tools all day!

Heterogeneity and plasticity of the naive CD4+ T cell compartment @cp-cellreports.bsky.social
www.cell.com/cell-reports...

Congrats Grayson!!

Thrilled to share the primary focus of my PhD thesis work, now out in @science.org today!

We developed Trikines — engineered cytokines that reconfigure receptor complex assembly to reshape signaling directly in endogenous immune cells.

t.co/UCixfysaUl

TCF1 is a master regulator of T cell stemness – but can it make exhausted cells go backwards?

We setup a CRISPR KI system to ask this question, and the answer was unexpected.

www.nature.com/articles/s41...

Ancestry and somatic profile indicate acral melanoma origin and prognosis - Nature Analysis of the somatic and transcriptomic profile of 123 acral melanoma samples from Mexican patients helps understand tumour origins and prognosis, and highlights the importance of including samples...

We are very happy to see our study finally appear online @nature.com! This has been work of nearly 10 years in collaboration with the National Institute of Genome Medicine 🇲🇽, the National Cancer Institute 🇲🇽, the @sangerinstitute.bsky.social and others ⬇️

www.nature.com/articles/s41...

Santiago-Carvalho et al. identified the extracellular ATP sensor P2RX7 as a poor prognostic factor in NSCLC that was highly enriched in Tregs. In mouse models, Treg-specific KO of P2RX7 reduced lung tumor burden, without altering Treg numbers. bit.ly/4quiPdW   @mayocliniccancer.bsky.social

Proteostasis sustains T cell differentiation potential and tumor-infiltrating lymphocyte function https://www.biorxiv.org/content/10.64898/2026.02.08.704716v1

Having Latin culture on FULL display quite literally makes me wanna cry.

#SuperBowl

Congrats Joe!!!

A little late on here but happy to share my 3rd first author paper from grad school finally out in Science Advances!! In collaboration with James Olzmann's group, we find that Tregs utilize FSP1/Aifm2 for preventing lipid peroxide accumulation and preserve their suppressive function in tumor models.

@biorxivpreprint.bsky.social The CD8 immgenT framework as a universal reference of mouse CD8 Tαβ cell differentiation states
www.biorxiv.org/content/10.6...

Atlas-guided discovery of transcription factors for T cell programming @nature.com @salkinstitute.bsky.social @tcellogic.bsky.social @ucsdmedschool.bsky.social
www.nature.com/articles/s41...

Selective Immune Silencing by Targeted TGF-β Agonists https://www.biorxiv.org/content/10.64898/2026.01.19.700410v1

A ‘simple’ experiment but an amazing gem of a reference!

Just stimulate blood, observe and describe…

#ImmunoSky