Davide Angeletti

How thoughtful experimental design can empower biologists in the omics era - Nature Communications Here, the authors discuss principles of experimental design that are relevant for all biology research, along with special considerations for projects using -omics approaches, highlighting common expe...

I am really excited to see this article on basic experimental design principles published. Definitely will be mandatory reading for incoming graduate students in my lab. Maggie Wagner who led this article did an amazing job with the illustrations of basic principles www.nature.com/articles/s41...

Virion motility of sialoglycan-cleaving respiratory viruses - npj Viruses npj Viruses - Virion motility of sialoglycan-cleaving respiratory viruses

New review paper from the influenza group
@utrechtvirology.bsky.social
together with Mengying Liu and Erik de Vries on virion motility of sialoglycan-cleaving respiratory viruses through mucus and on the surface cells in NPJ viruses @natureportfolio.nature.com
doi.org/10.1038/s442...

Preprint: Local B-cell immunity and durable memory following live-attenuated influenza intranasal vaccination of humans @profshanecrotty.bsky.social @hannahdstacey.bsky.social
www.biorxiv.org/content/10.1...

Replaying germinal center evolution on a quantified affinity landscape Darwinian evolution of immunoglobulin genes within germinal centers (GC) underlies the progressive increase in antibody affinity following antigen exposure. Whereas the mechanics of how competition be...

Wanted to highlight our latest preprint--a huge effort by multiple people and labs, but led primarily by @wsdewitt.github.io, Tatsuya Araki, and Ashni Vora, in a very close wet-dry collaboration with @matsen.bsky.social’s lab at the Hutch

www.biorxiv.org/content/10.1...

Adaptive immunity in barrier tissues Immune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

Last chance TODAY - to register and submit abstracts for #EMBObarrierTissues in Basel 🇨🇭🇨🇭🇨🇭! We've hit our registration goal 🥳, but there's always room for more. Join @maurogaya.bsky.social and me to explore the latest in lymphocyte and mucosal immunology. Details: meetings.embo.org/event/25-bar...

Congrats Danica 😀

Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase - npj Viruses npj Viruses - Kinetic MUNANA assay reveals functionally relevant antibody epitopes on Influenza A virus neuraminidase

Very proud of Danica Besavilla, PhD student in the lab, receiving the Poster award at the #Antibodies & Complement meeting, taking place in Catania.
She presented her paper on mAbs to #Influenza #Neuraminidase , just published in @natureportfolio.nature.com #npjViruses
www.nature.com/articles/s44...

Proud to announce our new technique for tracking relative B cell clonality in situ with B Cell Receptor MERFISH (BCR-MERFISH)! Congratulations to Evan Yang and our colleagues in the Carroll laboratory! Check out our bioRxiv: www.biorxiv.org/content/10.1... 1/9

<em>Immunology & Cell Biology</em> | ASI Journal | Wiley Online Library In this article for the Highlights of 2024 series, we review the latest advances in the biology of the germinal center response. These discoveries provide key insights into germinal center function a...

Our @immunolcellbiol.bsky.social "Highlights of 2024: Germinal Centers" is now out. Led by Theresa Pankhurst, we discuss some (there's never enough space for all!) neat germinal centre papers that were published in 2024, and whose findings are moving the field forward. doi.org/10.1111/imcb...

Postdoctoral position in Computational Biology open !

Our Lab is trying to understand the molecular and cellular mechanisms involved in establishing intestinal immune homeostasis and how a breakdown of these mechanisms may lead to diseases

More info 👇 :

lnkd.in/dQZq55nv

lnkd.in/d6sbw59P

The American Society for Microbiology is committed to ensuring that scientific research is conducted adhering to strict standards for biosafety and biosecurity, but pausing research is not the path forward. Read our statement: asm.org/press-releas...

Adaptive immunity in barrier tissues Immune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

🚨🦠🔬 Last month to register to the EMBO workshop on Adaptive Immunity in Barrier Tissues.

📍 Where: Basel, Switzerland
📅 When: 26th–29th August 2025
📝 Registration deadline: 30th May 2025

Childcare support & travel grants from @embo.org and @efis-immunology.bsky.social

#EMBOBarrierTissues

In @jem.org, Conlon, Huang, and Gerner show that immunization generates concentration gradients of antigens and #inflammation across interconnected chains of lymph nodes that regulate the magnitude and heterogeneity of adaptive immunity. rupress.org/jem/article/...

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination | PNAS The nasal mucosa is the first immunologically active site that respiratory viruses encounter and establishing immunity at the initial point of path...

Neat @pnas.org paper from @angelettilab.bsky.social & @matsbemark.bsky.social on ectopic germinal centres in the nasal turbinates of mice and humans. doi.org/10.1073/pnas...

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination @angelettilab.bsky.social
www.pnas.org/doi/10.1073/...

Thank you, Marty!

Thank you! This work opened up a lot of questions, which we hope to answer in future studies

Thank you @maurogaya.bsky.social , looking forward to the meeting!

And I forgot to thank the collaborators, without whom the work would have not been possible.
@matsbemark.bsky.social for continued discussions and key scientific input.
#KaWeiTang for swabs #JohanHellgren and team for tonsils
Other lab members: #NimithaMathew , #LauraReusch and #KarinSchön above all

We show that the same structures are also present at steady state, after vaccination and also in humans.
Our work adds to recent studies that identified DALT, nasal immunity and ectopic GC in skin.

We thank the funders @vetenskapsradet.bsky.social , @kawresearch.bsky.social and @erc.europa.eu !

Interestingly, another old paper described M cells in mouse nasal turbinates.
By sequencing we showed that germinal centers in nasal tissue were essentially transcriptionally identical, but clonally distinct, to those in cervical lymph nodes and NALT. (5/N)

But we found some old studies describing nasal tissue immunity after influenza infection in absence of NALT and cervical lymph nodes. Indeed, we could confirm ectopic germinal centers in the nasal turbinates by microscopy. Cells express AID, we also found Tfh and FDCs. (4/N)

By flow cytometry, a lot of these cells looked like germinal center B cells and followed the same dynamics as those in secondary lymphoid organs (and were antigen specific).
We were confused because we had carefully removed the NALTs before running them. (3/N)

First, Romain showed that by infecting with higher viral titer in lower volume we can increase viral replication in nasal tissue and also had more B cells within the cervical lymph node and nasal tissue itself (2/N)

Ectopic germinal centers in the nasal turbinates contribute to B cell immunity to intranasal viral infection and vaccination | PNAS The nasal mucosa is the first immunologically active site that respiratory viruses encounter and establishing immunity at the initial point of path...

Our latest study by PhD student #RomainGailleton shows that upon #influenza infection, #Bcell can respond rapidly by forming ectopic #GerminalCenter within the nasal tissue.
The work has just been published in @pnas.org (1/N)

www.pnas.org/doi/10.1073/...

Cool germinal center acrobatics from Juhee Pae et al, check it out!

www.nature.com/articles/s41...

Excited to share @joelrc.bsky.social 's new paper examining the factors underlying cell-to-cell heterogeneity in interferon induction during flu infection:

www.biorxiv.org/content/10.1...

Immune history shapes human antibody responses to H5N1 influenza viruses - Nature Medicine H5N1 strain-specific antibodies are higher in older individuals and correlate more with birth year than with age, suggesting that younger individuals are potentially more likely to benefit from H5N1 v...

Today @natmedicine.bsky.social published our study showing the older individuals born prior to 1968 have more antibodies that cross-react to #H5N1 relative to younger individuals. If H5N1 causes a pandemic, children will likely be the most susceptible. 1/2

www.nature.com/articles/s41...

Gut IgA-antibody secreting cells segregate into four Blimp1+ subsets based on differential expression of IgA and Ki-67 and are retained following prolonged αCD20 B cell depletion in mice Abstract. B cell depletion is an efficacious therapy for multiple sclerosis, but its long-term safety profile in the gastrointestinal tract has not been sp

academic.oup.com/jimmunol/adv...

Boosting human immunology: harnessing the potential of immune organoids | EMBO Molecular Medicine EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.

Very excited to share our new review in @embomolmed.org @embopress.org on human tonsil/adenoid-based immune organoid cultures for drug and vaccine testing in a human-first approach! With @maximilianmoll.bsky.social at @immunosens.bsky.social and @unibonn.bsky.social www.embopress.org/doi/full/10....