Jonathan Lenz

😍 Our research was featured in German radio yesterday by @quarkswdr.bsky.social !!

Listen here (starts at 1h07min): www1.wdr.de/mediathek/au...

@unimarburg.bsky.social

Also check out our press note (Pressemitteilung) in case you understand German🙂.

www.uni-marburg.de/de/aktuelles...

@unimarburg.bsky.social

(7)
This was such a fun project! Big thanks to everyone involved❤️!
@unimarburg.bsky.social @dfg.de @narjournal.bsky.social

(6)
In summary we found that Hang regulates transcription at the level of chromatin modification. It limits local H4K16ac deposition by NSL, thereby preventing overproduction of mRNA. We hypothesize that this fine-tuning of stress genes might play a role in the development of ethanol tolerance.

(5)
To address this, we studied the NSL-catalyzed histone mark H4K16ac in Hang knock-out cells. We found that H4K16ac increases at a fraction of TSSs upon Hang loss. The underlying genes are derepressed upon Hang depletion but recover their repressed status upon further removal of NSL.

(4)
We further investigated the interplay between Hang, a repressor, and the NSL complex, an activator of transcription. Both show robust interaction with each other (Co-IP). However, they have opposite effects on gene expression (RNA-seq upon RNAi). How can this be reconciled?

(3)
We showed that Hang binds to the transcriptional start sites of actively transcribed genes (ChIP-seq) and dampens their expression (RNA-seq upon RNAi). We think that this might protect cells from overshooting transcription of highly active genes.

(2)
Using a CRISPR/Cas9-based approach in a Drosophila cell line coupled with mass spectrometry, we found that Hang interacts with a broad spectrum of chromatin-regulating protein complexes (e.g. dREAM, Ino80, Sin3, dMec, TFIID, NSL).

(1)
The zinc finger protein Hangover (Hang) is implicated in stress responses in Drosophila. Particularly, it enables fruit flies to tolerate the effects of ethanol, which they are constantly exposed to living on rotting fruit. We investigated the molecular functions of Hang.

Hangover regulates gene expression by limiting NSL-mediated H4K16 acetylation Abstract. The RNA-binding protein Hangover (Hang) is essential for several stress responses in Drosophila melanogaster. Here, we discover a novel function

🚨Paper Alert🚨
We found a novel transcription factor function of the zinc finger protein “Hangover” in the regulation of Drosophila gene expression🪰🧬:

doi.org/10.1093/nar/...

#chromatin #transcription #hangover #histone modification #NSL complex

A thread:

Many thanks to all attendees of the GfG Sympsoium "Epigenetics & Chromatin" for making this conference such a great success! Go to www.gfgenetik.de for more information and fotos. Become a GfG member!

This was truly an amazing meeting😍! Rarely have I had such fruitful exchanges with exceptional scientists. Thanks so much to @gfgenetik.bsky.social and especially @hake-chromatin.bsky.social and team for providing this opportunity🙏.
I’m feeling the post-conference motivation high🤩.

Amazing 2. day at the GfG ‘Epigenetics & Chromatin’ symposium. Fantastic flash talks, a great first poster session and many memorable talks: among others @erhardtlab.bsky.social, @rodvilla.bsky.social, @thebraunlab.bsky.social, @flurylab.bsky.social, Ehrenhofer-Murray, Liefke, Heun, Imhof & Brehm.

Trump's Harvard cuts threaten a giant in biomedical research: A database about the tiny fruit fly This spring, the Trump administration rescinded a grant that maintained FlyBase, a pillar of global basic research, as part of its broader $2.2 billion funding cuts at Harvard.

The FlyBase situation has made the national news www.nbcnews.com/science/scie...

🎉 Join us at the upcoming GfG Symposium "Epigenetics & Chromatin" in Giessen, Sept 24–26, 2025!
Amazing speakers, low registration fee, € 300 prizes for best poster & short talk, + free GfG membership for non-members.

Please RT!

express.converia.de/frontend/ind...

“As you can appreciate in this heatmap:”

#Drosophila @flybase.bsky.social requires emergency funding:
"As it stands, by the end of July, 2025, there will be no future updates to #FlyBase, and in the worst case scenario access to the website will also be lost". Please help if you can!

There's a commentary on FlyBase with an update on FlyBase’s uptime and IP blocks :http://flybase.org/commentaries/2025_01/IPbans.html

(long thread incoming)
FlyBase’s servers have been experiencing an unprecedented amount of traffic recently, which has been causing the site to crash.

The GfG is delighted to reconnect with everyone here on Bluesky!

We're excited to announce our upcoming symposium, "Epigenetics & Chromatin", taking place September 24–26 in Giessen, Germany.
Stay tuned for more details!
(... and become a member of the GfG...!)

60 Hochschulen und Forschungseinrichtungen verlassen diesen Freitag die Plattform X/Twitter. idw-online.de/de/news845520 Die Uni Marburg ist auch dabei: uni-marburg.de/RMonrG #eXit #ByeByeX #QuitX Illustration: HHU/Paul Schwaderer

This looks amazing🤩!

Finally joined Bluesky and I'm so happy to find many #chromatin people on here! Let's go. 🧬🪰