Had a couple of good questions tonight:
Dad, what would happen if you threw a gallon of water on the sun?
What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?
Brief, sunny NYC trip, now to Boston for a few days
Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors
www.biorxiv.org/content/10.1...
Good afternoon for a jog past the @uoknightcampus.bsky.social
Hi @opazo.bsky.social no problem! Here are the antibodies and concentrations used:
T shirt and sunglasses day on the mountain
Spring skiing on Mt Hood
🌷Spring flowers? Fireworks?
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪
Three feet of powder means fun in the trees ❄️
For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.
🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.
🔗 www.janelia.org/news/new-met...
Mountain biking and skiing in the same weekend 🤘
Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪
Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪
Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬
Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine.
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊
FDA approves TROP2-targeted antibody–drug conjugate for breast cancer www.nature.com/articles/d41...
Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025
These mushrooms are also the source of phalloidin - just as toxic but a useful scientific tool to label the actin cytoskeleton of cells to view under a microscope
And more recently we introduced LysoLight Green! Happy imaging and enjoy the weekend.
LysoLight is ideally used in live cells; fixation is not recommended. Photostability is excellent, toxicity minimal, enabling long-term kinetic experiments.
Bright fluorescence is observed upon antigen-specific endosomal trafficking to the lysosome. Activation has been demonstrated with cathepsins B, D, & L. Inhibition is accomplished by blocking cathepsin, vATPase, or saturating cell-surface antigens with competing antibody.
The LysoLight family of dyes use a unique sensor that is activated by enzymatic cleavage specific to the lysosome, and therefore reports delivery of a protein or antibody to a functional lysosome. Minimal observable background fluorescence contributes to extraordinary sensitivity.
Several types of modern biotherapeutics require cellular uptake and lysosome delivery for efficacy. This includes ADCs, IgG-LNP, IgG-oligo, and of course extracellular targeted protein degraders like LYTACs. Existing fluorescence-based sensors report lysosomal delivery, but not catabolic activity.
🦠🔬 Recently we reported the development of a new class of sensors to directly monitor live-cell protein trafficking and provide a sensitive indication of delivery to functional, enzymatically active lysosomes. A quick 🧵for #FluorescenceFriday www.biorxiv.org/content/10.1...
Fascinating deep-dive into Roche’s new Sequencing By Expansion (SBX) technology
omicsomics.blogspot.com/2025/02/roch...
