@margaretbrisbin.bsky.social
Asst. Prof. / #newPI MICOlab @USFCMS studying interactions btwn marine microbes. ❤️ radiolarians, phytoplankton, bacteria. PhD from @oistedu. she/her. https://micolab-usf.github.io/home/
It is so amazing that we can study protists (single celled organisms) using an ROV. We collected one of these on the #LivingBioreactors cruise on the @schmidtocean.bsky.social R/V Falkor last month. It went to a colleague studying the chemistry of their calcium carbonate shells.
Amazing! The crew suggested this to me on my last cruise but I stuck with the small boat ops. Cool to see it actually done!!
Don’t know yet! Have a lot of sequencing and IDing work to do. We saw some weird tunicates, ctenophores, and clams - but I’m really the microbe person :)
What a missed opportunity - there was a ton of dry ice, bags of big tubes full of seawater, boxes of little tubes with filters from filtering seawater, and a few tubes with individual organisms in them that we want to ID, also a marine sediment core and little baggies of deep sea mud
Oh wow I should have done that.
Little shred of good news - box1 of the samples my colleagues and I collected near Antarctica have completed their long and arduous journey through customs and ports and airplanes and trucks to my lab. Now the real work starts!! 😬😬😬 #oceanography
🌊➡️🚢➡️✈️➡️🚛➡️👩🔬
Come to BIOS this fall for an undergraduate #NSFREU internship!
bios.asu.edu/education/ns...
📢 Now accepting proposals for the Graduate Research Excellence Grants! These provide evolutionary biology research funds for early and advanced Master’s and PhD students. Applicants must be members of SSE. Deadline: May 18, 2026
www.evolutionsociety.org/content/soci...
Next time ;)
Need instructors?
Protist lovers: Please apply for a week of rhapsody! Learn by doing:
MicroSEA (Microbial Sea Eukaryotes) course is a one-week, field-based summer school to train early-career researchers in the diversity, ecology, and single-cell biology of marine protists. opbc.sciencesconf.org
#protistsonsky
It is happening again! Consider taking our course on the Molecular and Cell Biology of Symbiosis at
@mblscience.bsky.social. Application deadline March 4th! www.mbl.edu/education/ad...
Nope not wrong. But also not exactly helpful.
Today, my mom asked me if I ever considered publishing in Science Magazine to reach a broader audience... Thanks, Mom. #oceanography 🌊🤦♀️🙄
Dinoflagellate reference in my Spotify feed today 🤓🌊🦠
Learn more and register for free virtual #NASA_PACE applications workshop here: pace.oceansciences.org/events_more.... 🌊
And we got sweet stickers :)
Our Antarctic cruise on the Sikuliaq (based in AK) hosted the Antarctic triple ARRR 100k challenge: run 🏃♀️, row, & ride 🚴♀️a total of 100k with at least 10k of each. Collectively, we completed 14,682 km, which is more than the distance Sikuliaq traveled from Alaska to Antarctica! #oceanography 🌊🇦🇶🐧
Who gets dizzy first?!
Wow! This is so inspiring. The things I see while bike commuting everyday ……
U.S.-based scientists are invited to apply for PSECCO Conference Travel Grant funding by March 15, 2026. Learn more about who is eligible to apply and how here: psecco.org/news/psecco-...
The term eDNA makes me seethe with rage.
I’ve literally been looking for something exactly like this.
If you figure it out tell me because that would speed up library prep considerably!
I’m not sure if that would work with how the kit is set up, but could probably be hacked. I imagine you would just need your own bioinformatics pipeline. The barcodes in the native barcode kit have some adapters and are auto recognized by the minknow software.
The native barcoding kit gives more flexibility - do whatever pcr you want and then the nanopore library prep and barcoding is pcr-free. We got this advice from the nanopore rep when we were choosing kits and because we wanted to do 18s too. But I do think adjusting cycle number willl work.
We actually haven’t been using the 16s kit, we have been using the native barcoding kit on our pcr products. Do you mean low input to the pcr or low input post pcr? We are low input pre-pcr.
Yes we do
