Bluesky handles: @biorxivpreprint.bsky.social‬ ‪@perrimonlab.bsky.social‬ @addgene.bsky.social‬ ‪@dgrc.bsky.social‬ @bdsc.bsky.social‬ @damonrunyon.org‬ Collaborators: ‪@aliceyting.bsky.social‬, Steve Carr, John Asara.

My lab is recruiting post-docs! We study inter-organ communication using cutting-edge tools like proximity labeling (TurboID), in silico modeling (AlphaFold), and genome engineering (CRISPR). Come join us @UofUGenetics, Utah is beautiful! (View from our fly room 👇)

a blood drop with a face and arms and legs ALT: a blood drop with a face and arms and legs

Huge thanks to my amazing co-authors, mentors, and lab mates! Also grateful to the @DRSC_TRiP and fly community for resources and support 🪰, @DamonRunyon and @NIH for funding.
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Plasmids 🧬, cell lines 🧫, and fly lines 🪰are available NOW from @Addgene, @DrosDGRC, and Bloomington Drosophila Stock Center (BDSC)! A web-based portal to visualize our snRNA-seq data is at www.flyrnai.org/scRNA/body_l...
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Why does this matter?💡
Mapping the sources of circulating proteins unlocks new ways to study biology and disease. This atlas can help others explore tissues (e.g. brain), proteins (e.g. Lectins), and processes (e.g. inter-organ communication) - or even discover biomarkers.
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Amazingly, we also found tissue-specific secreted proteins that accumulate in entirely different tissues! These may be remote-control factors that allow two organs to communicate (aka inter-organ communication).
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We discovered a gold mine of 535 circulating proteins! Highlights:
-Tissue-specific secreted proteins
-Unexpected sources like glia 🧠
-Shed ectodomains of transmembrane proteins
-Uncharacterized proteins
-Human-conserved proteins 🧬
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We built an atlas of secreted proteins in Drosophila blood from 10 different tissues using complementary ‘omics tools 🧬- transgenic TurboID proximity labeling, mass spectrometry, single nucleus RNA-seq, and CRISPR knock-in flies.
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Extracellular proteins are critical for basic biology (development, physiology, immunity, etc), and thousands circulate in our blood plasma🩸. We know they’re secreted from cells in different tissues… so… *which* tissues secrete *which* proteins?🤔 🤷
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Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking Secreted proteins regulate many aspects of animal biology and are attractive targets for biomarkers and therapeutics. However, comprehensively identifying the "secretome", along with their tissues of ...

I’m thrilled to share a @biorxivpreprint for my postdoc work in the @PerrimonLab🎉. Together with our collaborators, we built a tissue-specific atlas of circulating secreted proteins in Drosophila 🧬🪰🧪🗺️.
Thread below🧵
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www.biorxiv.org/content/10.1...

Post-docs - apply to speak at the Rising Stars in Genetics and Genomics Symposium at the University of Utah! Research areas include 1) evolutionary genetics and genomics, 2) functional and disease genetics, or 3) computational genomics docs.google.com/forms/d/e/1F...

Excited to be at fly meeting! #Dros25 🪰 Come by my poster #909F and see my talk #176. My lab is hiring! Including post-docs and lab techs. utah.peopleadmin.com/postings/179...