Huge thank you to all the co-authors for their contributions - @samlacey.bsky.social, Noor Verwei, @margotriggi.bsky.social, Jose Davila-Velderrain, @gaiapigino.bsky.social - and the fantastic support from @humantechnopole.bsky.social ✌️
Overall, the multi-step assembly process ensures coordinated emergence of structural integrity and motility. Functional modularity appears to be in-built, meaning assembly can easily be modulated to generate the diverse cilia-based structures we see across life.
There was so much to unpack with this data, we teamed up with the marvellous @margotriggi.bsky.social to help explain what we think is going on. (Please also check out our model of central apparatus assembly here: www.biorxiv.org/content/10.6...)
By imaging the tips of Chlamydomonas reinhardtii motile cilia by #cryo-ET, we captured the intermediate steps in axoneme assembly and uncovered a host of unexpected structures along the way.
Very happy to say that our work on how axonemes are generated is now available on bioRxiv: www.biorxiv.org/content/10.6... - if you’ve ever wondered how the incredibly beautiful, complex structures within motile #cilia are constructed then this is for you!
Many congratulations to Girish R Mali (@dyneinassembly.bsky.social) and team, awarded the 2025 Ian & Barbara Gibbons Award for their work on the regulation of axonemal dynein motors that power ciliary beating!
The award recognises groundbreaking advances in the Dynein field every four years.
Time to get signed up for the Symposium on Structural Proteomics! This time at @humantechnopole.bsky.social in Milan 6th-8th October Full speaker lineup announced. Thanks to @thermofishersci.bsky.social @brukercorporation.bsky.social, Affipro and MSVision for support
Info
ssp2025.squarespace.com
New preprint out 🔥🔥 on my postdoc work with @manuelthery.bsky.social and @lblanchoin.bsky.social about how we can create sustained dynamic steady states of actin network inside microscopic wells that contract "forever" without collapsing! A thread 🧵
www.biorxiv.org/content/10.1...
Interested in learning to process cryo-ET data in the latest version of Warp? I'll be running a workshop on April 22nd in central London, UK
details+registration: forms.gle/FLDr3ZWtVGXs...
Hope to see you there!
JOB ALERT!!! The National Facility for Structural Biology at @humantechnopole.bsky.social is HIRING!! BlueSky-less Andrea Graziadei is looking for a technician to join his Unit and push #structuralproteomics to new, uncharted territories!! 1/3
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪
Our review of Intraflagellar transport is out now! We cover lots of the recent advances in our understanding of IFT, and where we think there are still some gaps.
www.nature.com/articles/s41...
✨New online ✨
The intraflagellar transport cycle - written by Samuel Lacey & Gaia Pigino. The article beautifully summarises recent insights on transport trains - don’t miss the gorgeous structural detail!
www.nature.com/articles/s41...
