Pablo J. Sรกez's Avatar

Pablo J. Sรกez

@pjsaez.bsky.social

Husband - Dad of 2 - Scientist ๐Ÿ‡จ๐Ÿ‡ฑ Chilean expat. I love to study how cells walk & talk. PI of the @cellcommlab.bsky.social #CellMigration #CellComm #calciumsignaling #organelles ๐Ÿงช๐Ÿ”ฌ

1,594 Followers  |  469 Following  |  238 Posts  |  Joined: 24.08.2023
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Posts by Pablo J. Sรกez (@pjsaez.bsky.social)

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New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

04.03.2026 10:11 โ€” ๐Ÿ‘ 71    ๐Ÿ” 37    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 3
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PhD and Postdoc Transition Program | Gilliam Fellows | HHMI The Gilliam Fellows Program supports PhD students and their faculty thesis advisors to advance cutting-edge research, facilitate the transition to postdoctoral scientist, and foster the development of...

2nd- or 3rd-year PhD student? Applications for @hhmi-science.bsky.social's #GilliamFellows Program open 9/1! Robust dissertation support, an incredible community of scientists, tailored professional development (for you *and* your faculty thesis advisor), & so much more. Bookmark bit.ly/4qQArRh!

04.03.2026 21:10 โ€” ๐Ÿ‘ 9    ๐Ÿ” 9    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Contact/Job - Department of Molecular and Cellular Biology - UNIGE

You are finishing your PhD and looking to continue in science?

The Martin lab @biology-unige.bsky.social has an open postdoctoral position in cell biology to study cell-cell fusion. For more information, please consult mocel.unige.ch/research-gro....

Thanks for reposting!

03.03.2026 09:42 โ€” ๐Ÿ‘ 51    ๐Ÿ” 60    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 2
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Lรฉa Marpeaux, Claire Baudouin, Gregory Emery @emery-lab.bsky.social and colleagues @umontreal.ca identify an apical supracellular actin network in epithelial cells that acts as a long-range force transmission device.

journals.biologists.com/jcs/article/...

journals.biologists.com/jcs/article/...

03.03.2026 10:03 โ€” ๐Ÿ‘ 18    ๐Ÿ” 7    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 2
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Imaging spotlight: PhotoFiTT - FocalPlane Imaging spotlight: PhotoFiTT - News

๐Ÿ”ฌ๐Ÿ”ฆIn our latest โ€˜Imaging spotlightโ€™ Estibaliz Gรณmez de Mariscal @gomez-mariscal.bsky.social & Mario Del Rosario @mariodelr.bsky.social tell us all about PhototFiTT, the framework they developed for measuring phototoxicity in live-cell fluorescence imaging.
focalplane.biologists.com/2026/03/02/i...

02.03.2026 13:13 โ€” ๐Ÿ‘ 11    ๐Ÿ” 5    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Apply to become a Preprint Editor
Development

Apply to become a Preprint Editor Development

We are inviting experienced researchers to apply to become Preprint Editors, a new type of editorial role responsible for handling our 'In preprints' articles and forging links with preprint literature. #prepints

Learn more and apply before 30 March 2026:
journals.biologists.com/dev/pages/pr...

02.03.2026 14:55 โ€” ๐Ÿ‘ 12    ๐Ÿ” 16    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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We had covered the history of traction force microscopy (TFM) earlier:
bsky.app/profile/epim...

But what about applying TFM into more physiologically relevant systems, such as those in 3D?

I am @barrasa-fano.bsky.social and I'll be your guide through this thread on #3DTractionForceMicroscopy.

01.03.2026 07:58 โ€” ๐Ÿ‘ 22    ๐Ÿ” 11    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 2

One week left to register to the Bioimage Analysis with AI course in Pasteur!

This June, 1 - 5.

I give a bit more details in the thread below
๐Ÿ‘‡

24.02.2026 19:48 โ€” ๐Ÿ‘ 39    ๐Ÿ” 29    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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Have you produced beautiful images as part of your cell biology research?

Then enter our image competition!

bscb.org/competitions...

Deadline 31st March

Check out last year's winner 'Brainbow' by Alvaro Castano

25.02.2026 16:37 โ€” ๐Ÿ‘ 13    ๐Ÿ” 8    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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๐Ÿ”ฌ๐Ÿ”ดJoin us for the 9th Danish Bioimaging Symposium on May 7-8 at the Danish Cancer Institute
Donโ€™t miss it!. Registration is now Open!
The symposium will emphasize advancing cancer research through imaging technologies.
Sections: Image Analysis, Microscope Techniques and Scientific Cancer research.

19.02.2026 11:41 โ€” ๐Ÿ‘ 10    ๐Ÿ” 5    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Microscopy preprints โ€“ new tools and techniques in imaging - FocalPlane Microscopy preprints โ€“ new tools and techniques in imaging - News

Our latest preprint list is up on FocalPlane.

This list is focussed on new tools and techniques in imaging. Let us know if you have any recommendations for us to add.

focalplane.biologists.com/2026/02/20/m...

20.02.2026 12:09 โ€” ๐Ÿ‘ 8    ๐Ÿ” 5    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Join us next Tuesday for the next @dgz.bsky.social workshop on #trafficking and EVs! Spread the word please!

20.02.2026 14:25 โ€” ๐Ÿ‘ 17    ๐Ÿ” 15    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 4
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Cell body getting taken for a ride in this early stage neuron. Microtubules๐Ÿ”ต and Actin๐ŸŸฃ labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday

20.02.2026 14:30 โ€” ๐Ÿ‘ 54    ๐Ÿ” 10    ๐Ÿ’ฌ 2    ๐Ÿ“Œ 2
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Activities - GreenTE Activities Webinars โ€MechanoBioTalks โ€“ Mechanics, Materials and Living Matterโ€ is a webinar series supported by the GreenTE consortium and organized [โ€ฆ]

We are happy to announce the start of the MechanoBioTalks โ€“ Mechanics, Materials and Living Matter an initiative organized by GreenTE young researchers. The aim of the webinars is to bring together researchers interested in biomechanics, biophysics and biology.
Register here: green-te.nl/activities/

22.01.2026 09:29 โ€” ๐Ÿ‘ 9    ๐Ÿ” 5    ๐Ÿ’ฌ 2    ๐Ÿ“Œ 1

Looking forward to hear what you find.
Good luck

07.02.2026 22:02 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Postdoctoral Fellow - Cellular signalling and cytoskeletal function lab

2nd postdoc job alert in the Crick Institute in London. Project will examine the role of motors & the cytoskeleton (actin, septins and microtubules) during vaccinia virus egress using biochemical, structural & cellular approaches + live cell imaging. spread the word.
crick.ac.uk/WayPostdoc20...

06.02.2026 08:29 โ€” ๐Ÿ‘ 30    ๐Ÿ” 40    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1

Very nice imaging !
It seems that some areas of the brain are somehow depleted of microglia.
Is that correct? And if so, who takes over the immunosurveillance in those regions?

07.02.2026 09:16 โ€” ๐Ÿ‘ 1    ๐Ÿ” 0    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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Not something you see in textbooks very often: tripolar mitosis.

07.02.2026 08:00 โ€” ๐Ÿ‘ 1576    ๐Ÿ” 275    ๐Ÿ’ฌ 50    ๐Ÿ“Œ 22
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Microscopy preprints: bioimage analysis - FocalPlane Microscopy preprints: bioimage analysis - News

Our #preprint list is up on FocalPlane. This list focusses on the latest tools in bioimage analysis.
Let us know if you have any recommendations to add to our list.
focalplane.biologists.com/2026/02/06/m...

06.02.2026 14:55 โ€” ๐Ÿ‘ 19    ๐Ÿ” 12    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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"The microtubule GTP-tubulin cap size is modulated during cell division" is now available on @biorxivpreprint! This video by Anna Cassidy shows the tips of growing microtubules (EB1) and DNA (histone 2B) during metaphase. #Cellbiology
www.biorxiv.org/content/10.6...

04.02.2026 17:20 โ€” ๐Ÿ‘ 36    ๐Ÿ” 7    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 1
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BINA STED User Group, February 12, 12 PM ET - FocalPlane BINA STED User Group, February 12, 12 PM ET -

The next @bioimagingna.bsky.social @canadabioimaging.bsky.social Nanoscopy (previously STED) User Group meeting is on 12 February from 12 pm ET. The group welcomes researchers at all levels for an open discussion on nanoscopy methods.
focalplane.biologists.com/2026/02/02/b...

03.02.2026 16:46 โ€” ๐Ÿ‘ 5    ๐Ÿ” 2    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
Fill out our community survey to help shape the future of JCS

Journal of Cell Science logo

Line drawing of a group of people with speech bubbles above their heads

Fill out our community survey to help shape the future of JCS Journal of Cell Science logo Line drawing of a group of people with speech bubbles above their heads

After 15 years as Editor-in-chief (EiC), Michael Way @drmichaelway.bsky.social will be stepping down at the end of 2026 & we are seeking feedback from the cell biology community as part of the consultation process for appointing a new EiC.
We'd love to hear from you: www.surveymonkey.com/r/GZCC9F9

03.02.2026 13:36 โ€” ๐Ÿ‘ 23    ๐Ÿ” 26    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 1
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#FluorescenceFriday

Jost, Schweizer et al. @ Cuello lab found that a mutation linked to aortic aneurysmas led to distorted actin #cytoskeleton in VSMCs #mechanobiology

New collab @cellcommlab.bsky.social by @jboixcampos.bsky.social๐Ÿ”ฅ

Full www.sciencedirect.com/science/arti...
#microscopy #cell ๐Ÿงช๐Ÿ”ฌ

16.01.2026 21:43 โ€” ๐Ÿ‘ 31    ๐Ÿ” 8    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0
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JCS snapshot - Tracking coordinated cellular dynamics in time-lapse microscopy with ARCOS.px

@macdobry.bsky.social presents the key findings from their recent JCS paper with @bgraedel.bsky.social and colleagues @olivierpertz.bsky.social.

journals.biologists.com/jcs/article/...

29.01.2026 14:24 โ€” ๐Ÿ‘ 22    ๐Ÿ” 9    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 1
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#Addendum to 1970 paper, โ€œCYTOPLASMIC FILAMENTS OF AMOEBA PROTEUS: I. The Role of Filaments in Consistency Changes and Movement,โ€ includes eight video sequences originally recorded on 16-mm films. No technology was available at the time to include this data. ๐ŸŽž๏ธ rupress.org/jcb/article/...

#Actin

29.01.2026 15:45 โ€” ๐Ÿ‘ 24    ๐Ÿ” 7    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 1
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T cell polarization and NFAT activation are stiffness dependent and differentially regulated by the channels PIEZO1 and ORAI1 Target stiffness influences T cell polarization and gene expression through distinct Ca2+ channels.

Very hot from the press !

Almost every topic we work in:
#IonChannels #Leukocytes #CalciumSignaling

T cell response depends on target-cell stifness to drive Piezo1- Orai1 membrane channels activation, softening of tumours prevents this response.

Full www.science.org/doi/10.1126/... @science.org

27.01.2026 20:17 โ€” ๐Ÿ‘ 31    ๐Ÿ” 5    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0

Wow !!! Congratulations to @manuelthery.bsky.social and all the Cytomorpho lab !!
This is the Living architectures at the top of the top !!
I wished I was there to see it in live...

Proud to have contributed with a tiny part of one of the previous versions.

25.01.2026 23:34 โ€” ๐Ÿ‘ 4    ๐Ÿ” 0    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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CELL MIGRATION LAB We study cell migration in health and disease

๐Ÿšจ We are hiring TWO postdocs to join the Cell Migration Lab (cellmig.org) ๐Ÿ‡ซ๐Ÿ‡ฎ as part of the new Centre of Excellence in Immuneโ€“Endothelial Interfaces (IMMENs).
Join us to decode immune regulation & develop next-gen imaging tools! ๐Ÿงช๐Ÿ”ฌ
Details in thread ๐Ÿงต๐Ÿ‘‡
#ScienceSky #Postdoc #AcademicJobs Please RT๐Ÿซถ

21.01.2026 07:57 โ€” ๐Ÿ‘ 71    ๐Ÿ” 65    ๐Ÿ’ฌ 3    ๐Ÿ“Œ 4
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Skin cell volume regulates their terminal differentiation

๐Ÿ“ท Sebastiaan Zijl et al @kingscollegelondon.bsky.social
in @jcellsci.bsky.social
โžก๏ธ bpod.org.uk/archive/2026... with Lux Fatimathas

15.01.2026 09:53 โ€” ๐Ÿ‘ 4    ๐Ÿ” 1    ๐Ÿ’ฌ 0    ๐Ÿ“Œ 0
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Interested in cell-cell communication and microscopy? We are searching for a motivated postdoctoral researcher to join Mรผnster! Please Apply! @uni-muenster.de

19.01.2026 16:54 โ€” ๐Ÿ‘ 22    ๐Ÿ” 24    ๐Ÿ’ฌ 1    ๐Ÿ“Œ 0