Doudna Lab

Tiny CRISPR Tool Opens the Door to Faster, Simpler Plant Genome Editing Tiny CRISPR Tool Opens the Door to Faster, Simpler Plant Genome Editing - Innovative Genomics Institute (IGI)

In a recent paper, IGI's @jenniferdoudna.bsky.social @doudna-lab.bsky.social and Jill Banfield @banfieldlab.bsky.social collaborate with UCLA's @jacobsenucla.bsky.social to create a new, tiny #CRISPR tool for quicker, easier plant genome editing! Read more in this Q&A with Steve: ow.ly/6Szs50VGElf

‼️ New pre-print from co-leads @owentuck.bsky.social and Jason Hu! Check out this fascinating example of how coevolution enables defense system innovation.

Our conversation photo and photo of Baby KKJ

A new Pediatric Center for CRISPR Cures builds on the recent saved life of Baby KJ, who expeditiously had bespoke in vivo base genome editing
@jenniferdoudna.bsky.social @doudna-lab.bsky.social
@innovativegenomics.bsky.social
@chanzuckerberg.bsky.social
erictopol.substack.com/p/new-center...

Kevin Wasko (left) and Honglue Shi in front of their gel imager, confirming that the SpRY Cas9 variant struggles to effectively open DNA

“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author Honglue Shi of the @doudna-lab.bsky.social on his recent paper in Molecular Cell

Read here: innovativegenomics.org/uncovering-c...

Baby KJ looking alert and healthy

Thanks to the BBC's The Inquiry program for great conversations with IGI's @jenniferdoudna.bsky.social @doudna-lab.bsky.social and @urnov.bsky.social about Baby KJ, #CRISPR on-demand, and the future of personalized medicine!

Listen here: www.bbc.com/audio/play/w...

Photo credit: TODAY

Registration is open for the 2025 Computational Structural Virology Symposium, featuring Doudna lab postdoc @jnoms.bsky.social!

Hello everyone! I am pleased to share information on the first ever Computational Structural Virology Symposium, conducted August 4th on zoom and highlighting work in this emerging field. You can register for this event here: forms.gle/CNiqskMwQEuV.... Please re-post!

US supercomputer named after Nobel laureate Jennifer Doudna to power AI and scientific research A new supercomputer named after a winner of the Nobel Prize in chemistry will help power artificial intelligence technology and scientific discoveries from a perch in the hills above the University of...

US supercomputer named after Nobel laureate Jennifer Doudna @jenniferdoudna.bsky.social @innovativegenomics.bsky.social to power AI and scientific research
apnews.com/article/nvid...

Congratulations to Matt on this incredible accomplishment! 🎉This K08 will allow him to continue developing CRISPR based therapies for children with inherited immune diseases.

Elated to share that I was awarded a K08 clinician-scientist research career development award from the National Institutes of Allergy and Infectious Diseases!

Congrats to Doduna lab postdoc @erinedoherty.bsky.social for being selected as a Leading Edge Fellow! @leadingedgeprogram.bsky.social

Congrats to Dr. Honglue Shi @honglue.bsky.social on his latest research! The importance of weak, yet specific, interactions 👀

@doudna-lab.bsky.social @cellpress.bsky.social

A photo of a mix of green and white Arabidopsis thaliana seedlings. They turn white when a particular gene is knocked out. Here, a tiny CRISPR system was used to modify the mother plant’s DNA, which the white seedlings inherited.

In a new paper, IGI's Jennifer Doudna @doudna-lab.bsky.social and Jill Banfield @banfieldlab.bsky.social collaborate with UCLA's @jacobsenucla.bsky.social to create a new, tiny #CRISPR tool for quicker, easier plant genome editing! Read more: ow.ly/6Szs50VGElf

YouTube video by Cleo Abram You Can Fix Your DNA... Starting Now (feat. Nobel Prize Winner)

Jennifer Doudna @jenniferdoudna.bsky.social @doudna-lab.bsky.social speaks with Cleo Abrams on the history and future of #CRISPR 🧬. Watch here: youtu.be/0OXaanDHENI?...

New Heroes portrait of @jenniferdoudna.bsky.social for the @nationalacademies.org. @doudna-lab.bsky.social explorers.com/jennifer-dou...

Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce...

Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!

Pictured: Kevin Wasko (left) and Honglue Shi in front of their gel imager, confirming that the SpRY Cas9 variant struggles to effectively open DNA

“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author @honglue.bsky.social of the @doudna-lab.bsky.social on his new paper out today in Molecular Cell

Read here: shorturl.at/p664D

Catch @honglue.bsky.social at the Harvard Genome Engineering Seminar Series this Monday, April 7 at 1pm EST / 10am PST! If you’ve ever wondered why SpyCas9 is so efficient at genome editing, don’t miss his talk—Zoom link: harvard.zoom.us/j/94394339529

🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪

@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇

A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...

Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.

Check it out:

Packaged delivery of CRISPR–Cas9 ribonucleoproteins accelerates genome editing Abstract. Effective genome editing requires a sufficient dose of CRISPR–Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune res

New from @doudna-lab.bsky.social in @narjournal.bsky.social: "Packaged delivery of #CRISPR–Cas9 ribonucleoproteins accelerates genome editing"

Read here: shorturl.at/AcZ8i

🧪🧬

Ben Adler & Muntathar Jamal Al-Shimary

Phage genomes are full of mysterious proteins that could be harnessed to create new genomic tools. IGI's @doudna-lab.bsky.social & @cresslab.bsky.social with co-first authors @benadler.bsky.social & Muntathar Jamal Al-Shimary detail a new way to exploring phage genomes: ow.ly/uucL50V77n0 🧪🦠🧬

squirrel in the woods

In a recent @naturecomms.bsky.social paper, @doudna-lab.bsky.social at the IGI & Michael Jordan’s lab in Cal’s Dept of Electrical Engineering & Computer Science unveil a new #AI-based method to narrow down large datasets of proteins to prioritize what to study in the lab: ow.ly/nxxn50V80fo

This week, I’ll highlight two recent CRISPR advances: 1) BEAM’s exciting Phase 1 clinical data for BEAM-302. 2) Impressive work from the labs of Scribe’s co-founders @savagecatsonly.bsky.social & @doudna-lab.bsky.social —providing a textbook example of engineering CRISPR enzymes from zero to hero.

We are so excited to bring more talented students to the IGI!

Pictured: Cydni Bolling of Hampton University, who did research in our lab last summer as part of the UC-HBCU Initiative. A special shout-out to her mentor postdoc @erinedoherty.bsky.social who connected IGI with the Initiative.

Single-molecule live-cell RNA imaging with CRISPR–Csm - Nature Biotechnology Single-molecule live-cell fluorescence in situ hybridization uses an RNA-targeting CRISPR–Csm complex to image and track endogenous RNAs.

Single-molecule live-cell RNA imaging with CRISPR–Csm - @doudna-lab.bsky.social @qb3-berkeley.bsky.social @innovativegenomics.bsky.social go.nature.com/3EJPKsM

Today in #journalclub, we looked at this fantastic new method from the @doudna-lab.bsky.social in Nature Biotechnology! They show how CRISPR–Csm enables single-molecule live-cell imaging of endogenous RNAs. Super cool!
www.nature.com/articles/s41...

CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d - Nature Microbiology Leveraging RNA-targeting dCas13d enables selective interference with phage protein translation and facilitates measurement of phage gene fitness at a transcriptome-wide scale.

🧬OUT NOW Functional Genomics of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi by @benadler.bsky.social @doudna-lab.bsky.social @cresslab.bsky.social

www.nature.com/articles/s41...

CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d - Nature Microbiology Leveraging RNA-targeting dCas13d enables selective interference with phage protein translation and facilitates measurement of phage gene fitness at a transcriptome-wide scale.

It is finally out!

Muntathar Al-Shimary, @doudna-lab.bsky.social , @cresslab.bsky.social and I present a gene knockdown tool that works in diverse bacteriophages: CRISPRi through antisense RNA Targeting (CRISPRi-ART)!

Check it out @naturemicrobiol.bsky.social
www.nature.com/articles/s41...

Register to attend the 25th annual Miller Symposium hosted by the UCD Dept. of Chemistry! Don't miss the chance to listen to the distinguished speakers, including Nobel Laureates @doudna-lab.bsky.social & @francesarnold.bsky.social
Click here to register and see the full lineup: lnkd.in/dpPHAW2