What happens at a UN climate summit? Here's a quick tour of the #COP30 venue to give a sense of the various meetings and activities.

Anna Possi has been brewing espressos and serving coffees in northern Italy for more than 80 years.

Possi turned 101 on Sunday. She has no intention to retire.

"I have customers who are now grandparents and come in with their grandchildren," Possi said.

BREAKING: Magistrate orders DOJ to turn over grand jury materials to Comey, finding real prospect that "government misconduct" may have tainted the case.

Asbestos waste is killing a remote First Nations community – and the WA government has known for decades A powerful new film exposes how deadly asbestos is destroying lives and land on Banjima Country

Asbestos has been killing Banjima people since the mine was closed in 1978 but WA gov keeps resisting doing what’s needed.
open.substack.com/pub/deepcutn...

Summary A team of scientists discovered a hidden antibiotic 100 times stronger than existing drugs against deadly superbugs like MRSA. The molecule had been overlooked for decades in a familiar bacterium. It shows no signs of resistance so far, offering hope in the fight against drug-resistant infections and paving the way for new approaches to antibiotic discovery.

Abstract The methylenomycins are highly functionalized cyclopentanone antibiotics produced by Streptomyces coelicolor A3(2). A biosynthetic pathway to the methylenomycins has been proposed based on sequence analysis of the proteins encoded by the methylenomycin biosynthetic gene cluster and the incorporation of labeled precursors. However, the roles played by putative biosynthetic enzymes remain experimentally uninvestigated. Here, the biosynthetic functions of enzymes encoded by mmyD, mmyO, mmyF, and mmyE were investigated by creating in-frame deletions in each gene and investigating the effect on methylenomycin production. No methylenomycin-related metabolites were produced by the mmyD mutant, consistent with the proposed role of MmyD in an early biosynthetic step. The production of methylenomycin A, but not methylenomycin C, was abolished in the mmyF and mmy mutants, consistent with the corresponding enzymes catalyzing the epoxidation of methylenomycin C, as previously proposed. Expression of mmyF and mmyo in a S. coelicolor M145 derivative engineered to express mmr, which confers methylenomycin resistance, enabled the resulting strain to convert methylenomycin C to methylenomycin A, confirming this hypothesis. A novel metabolite (premethylenomycin C), which readily cyclizes to form the corresponding butanolide (premethylenomycin C lactone), accumulated in the mmyE mutant, indicating the corresponding enzyme is involved in introducing the exomethylene group into methylenomycin C.

Remarkably, both premethylenomycin C and its lactone precursor were one to two orders of magnitude more active against various Gram-positive bacteria, including antibiotic-resistant Staphylococcus aureus and Enterococcus faecium isolates, than methylenomycins A and C, providing a promising starting point for the development of novel antibiotics to combat antimicrobial resistance.

Graphical Abstract

Chemists discovered a powerful hidden antibiotic that’s 100 times stronger than existing ones and effective against deadly superbugs.

The study has been published in the Journal of the American Chemical Society. YES, it is PEER-REVIEWED.
• pubs.acs.org/doi/10.1021/...

🧪🧵⬇️

Powerful new antibiotic that can kill superbugs discovered in soil bacteria Surprise discovery could pave the way for new treatments against drug-resistant infections.

This is good news
www.nature.com/articles/d41...

"Remarkably, the bacterium that makes methylenomycin A and pre-methylenomycin C lactone -- Streptomyces coelicolor -- is a model antibiotic-producing species that's been studied extensively since the 1950s. Finding a new antibiotic in such a familiar organism was a real surprise." 🧪

Jeffrey Epstein Leaned on His Elite Network for Help as Investigators Closed In A cache of 18,000 messages shows the elite support the disgraced financier got in his “hour of terror.”

As investigators closed in, elite experts and professionals offered support and advice to Jeffrey Epstein. A cache of private emails obtained by Bloomberg News reveals details from his network.

A MUST SEE

There are people who voted for Donald Trump because he promised to expose the Epstein files. Now there is real voter remorse.

Epstein survivor Haley Robson is one of them, and if she could speak to Trump today, she would say, “Who owns you?”

rabbit hole du jour

New: Only 2% of people DHS arrested in the Chicago area had a criminal record. Check out the whole video on Substack. substack.com/@thefrankfig...

It’s up to all of us to do our part to protect our democracy from authoritarian power grabs.

Twenty-two Trump-branded real estate projects will be developed in foreign countries during Trump’s presidency - CREW | Citizens for Responsibility and Ethics in Washington Over the course of Trump's second term, real estate developers will be working on developing Trump-branded projects around the globe.

Trump now profits from 22 foreign developments, several tied to gov land or state-linked partners. That gives foreign officials leverage over him. Our assessment warns when executive decisions overlap with private revenue streams, democratic checks weaken.

www.citizensforethics.org/reports-inve...

Natural forests of the world – a 2020 baseline for deforestation and degradation monitoring - Scientific Data Scientific Data - Natural forests of the world – a 2020 baseline for deforestation and degradation monitoring

We can map the world's existing forests with incredible accuracy now, but clearly we cannot (or will not) stop them from disappearing at rapid rates almost everywhere. www.nature.com/articles/s41... 🌎

If you read my stuff, you know I regularly go on about the Far Right being an interconnected, global threat that transcends borders.

How it probably happened:

Trump: I can’t make this Epstein mess go away. Even some republicans are starting to cave. Ungrateful cowards. What would they do without me and my rich friends?

Bondi: It will not come to that.

1/5

Oooooooooo—seems Koala may be the closest living relative to Thylacoleo…

The Liberal Party has lost the Australian business sector with its “sheer fatuousness and contradictions” by abandoning net zero 2050. AFR editorial 15-16/11/25. The so called party of rational market economics relegates itself to the political fringe. 👇✅

It's too easy to be distracted by the economic story of the day. The more important story is of our system fraying: The rule of law is decaying, Bribery spreading, crony capitalism normalized. It won’t show up in next quarter’s data, but when our kids try to build their lives.

Weather Monitor: A large amount of sea foam was spotted in Loughshinny this evening, just outside Dublin, Ireland. (©️CarlowWeather )

ladies and gentlemen, the weekend

find someone who will wear a big overcoat with you

IG meetcutesnyc

Senile Satan just dumped MTG

Mining billionaire Gina Rinehart the daughter of the man that said indigenous people in Australia should be bred out and the political leader of the most racist section of British politics smiling together. This can only be bad #auspol #nzpol

Ahem. It's the job of the President to appoint DAs - with advice and consent from the Senate.

The President can ignore that rule - but only once (per district) and only for 120 days.

Do you know who appoints DAs after the 120 day mark has passed?

Pam Bondi doesn't. Because it's the judiciary.

Count the ways they’re corrupting DOJ: Presidents don’t direct AG’s to open criminal cases, especially ones designating only Dems for investigation when POTUS himself is involved. DOJ doesn’t publicize criminal investigations & the AG definitely doesn’t assign them on Twitter.

Johnson Legal is proud to be representing Friends of Australian Rock Art in their internationally significant challenge to Woodside’s massive North West Shelf gas extension project.

Shock intervention as UN enters Woodside LNG battle www.theaustralian.com.au/nation/un-ra...

When Massachusetts passed a "millionaires tax" in 2023, conservatives claimed the rich would flee.

But two years later, they haven't — and MA has collected $5.7B for infrastructure and public education.

A reminder that positive change can still happen at the state level.

Nobel Laureate economist Joseph Stiglitz warns of 'inequality emergency' - ABC listen In 1966, Nobel Laureate Joseph Stiglitz wrote his PhD thesis on inequality. Almost sixty years later, after decades of research, numerous books, and international acclaim, he has led a new report for ...

Nobel Laureate economist Joseph Stiglitz warns of 'inequality emergency'.
www.abc.net.au/listen/progr...