Noah Gurley's Avatar

Noah Gurley

@noahgurley.bsky.social

PhD Student in the Biochemistry, Cellular and Molecular Biology Graduate Program at Johns Hopkins University School of Medicine. Currently rotating in the Seydoux Lab. Interested in coordinated cell behaviors, microscopy, social justice, and more.

339 Followers  |  693 Following  |  1 Posts  |  Joined: 12.11.2024  |  2.177

Latest posts by noahgurley.bsky.social on Bluesky

Huge shout out to @jenevievenorton.bsky.social, Erika, Sarah, @noahgurley.bsky.social, Rebecca, Emmanuel, Taino, the Jones Lab ...and of course my great mentor and PI Mark Peifer @peiferlabunc.bsky.social !!!

30.01.2025 16:24 — 👍 3    🔁 2    💬 0    📌 0
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Wow! We got the cover! #MBOC #ASCB #CellBio
So proud of #TeamTestis. Screening <270 lines for shape defects was a huge team effort!

I created this collage from the team’s images from the past 2 years! Depth-color-coded with Fiji. Inspired by late 19th century lithographic animal illustrations.

30.01.2025 16:17 — 👍 112    🔁 17    💬 8    📌 3
Immunofluorescence images of embryos showing that CnoRA1 domain is important for dorsal closure, head involution and epidermal integrity. Wild-type (WT) embryo. Some cells are rounded up to divide (white arrows), but they rapidly return to columnar architecture. (B) cnoΔRA1. Groups of cells near the ventral midline fail to resume columnar architecture (red arrows). (C–E) Stage 11. (C) Wild-type embryo. (D,E) cnoΔRA1. Failure to resume columnar architecture becomes more apparent (red arrows). (F–H) Stage 14. (F) Ventral view of a wild-type embryo. Dorsal closure is completed (white arrow), head involution is underway (yellow arrow) and segmental grooves are shallow (green arrowheads). (G) cnoΔRA1. Dorsal closure failed, exposing underlying tissues (red arrow). There are gaps in the head epidermis (yellow arrows) and deep segmental grooves remain (green arrowheads). (H) Ventral view, cnoΔRA1. Holes in the epidermis (red arrows) and deep segmental grooves (green arrowheads) are observed. (I–J) Closeups, wild-type stage 10 (I–I″) and 11 (J). Dividing cells (green arrows) and forming tracheal pits (cyan arrows) are observed. Arm, Baz and Cno remain enriched at AJs. (K–K‴) Stage 10/11 cnoΔRA1 mutant. Cells that retained columnar architecture retain junctional Arm, Baz and CnoΔRA1 (yellow arrows). However, in some cells AJs are fragmented (red arrows) and in less epithelial regions Arm, Baz and Cno are strongly reduced (cyan arrows).

Immunofluorescence images of embryos showing that CnoRA1 domain is important for dorsal closure, head involution and epidermal integrity. Wild-type (WT) embryo. Some cells are rounded up to divide (white arrows), but they rapidly return to columnar architecture. (B) cnoΔRA1. Groups of cells near the ventral midline fail to resume columnar architecture (red arrows). (C–E) Stage 11. (C) Wild-type embryo. (D,E) cnoΔRA1. Failure to resume columnar architecture becomes more apparent (red arrows). (F–H) Stage 14. (F) Ventral view of a wild-type embryo. Dorsal closure is completed (white arrow), head involution is underway (yellow arrow) and segmental grooves are shallow (green arrowheads). (G) cnoΔRA1. Dorsal closure failed, exposing underlying tissues (red arrow). There are gaps in the head epidermis (yellow arrows) and deep segmental grooves remain (green arrowheads). (H) Ventral view, cnoΔRA1. Holes in the epidermis (red arrows) and deep segmental grooves (green arrowheads) are observed. (I–J) Closeups, wild-type stage 10 (I–I″) and 11 (J). Dividing cells (green arrows) and forming tracheal pits (cyan arrows) are observed. Arm, Baz and Cno remain enriched at AJs. (K–K‴) Stage 10/11 cnoΔRA1 mutant. Cells that retained columnar architecture retain junctional Arm, Baz and CnoΔRA1 (yellow arrows). However, in some cells AJs are fragmented (red arrows) and in less epithelial regions Arm, Baz and Cno are strongly reduced (cyan arrows).

@emily-mcparland.bsky.social, @noahgurley.bsky.social, Kevin Slep, @peiferlabunc.bsky.social and colleagues dissect the differential roles of the dual Ras-association domains of Drosophila Canoe in linking cell junctions to the cytoskeleton.
journals.biologists.com/jcs/article/...

17.12.2024 13:06 — 👍 14    🔁 6    💬 1    📌 0
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Next to my talk (12/16 4 pm room 33B), I will present a poster: "Semaphorin/Plexin Antagonism Orchestrates Collective Cell Migration in vivo". Tuesday, Dec 17 11:15 AM.

P2780/Board No. B397

I am so excited. I just love ASCB meetings!

#cellbio2024 #cellbio #devcell @ascbiology.bsky.social #ASCB

12.12.2024 15:05 — 👍 17    🔁 6    💬 2    📌 0
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#FluorescenceFriday for my first post, of course. Developing chicken embryo transfected with pCIG on the left (green) and pCI H2B-RFP on the right (red) and co-stained for SOX10 (magenta) and PAX7 (cyan).

23.11.2024 01:46 — 👍 16    🔁 1    💬 1    📌 1

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