🚨 New preprint from our labs (STOP lab @stoplab.bsky.social & Nojima lab @pol2rna.bsky.social @pyrolyn.bsky.social)! We've been digging into how chromatin remodeler SETD2 controls the start and end of transcription 👇 www.biorxiv.org/cgi/content/... (1/5)

Range extender mediates long-distance enhancer activity - Nature The REX element is associated with long-range enhancer–promoter interactions.

Our paper describing the Range Extender element which is required and sufficient for long-range enhancer activation at the Shh locus is now available at @nature.com. Congrats to @gracebower.bsky.social who led the study. Below is a brief summary of the main findings www.nature.com/articles/s41... 1/

Join us next Wed, for three amazing ECR researchers_ @fueyoraquel.bsky.social, @kaiamattioli.bsky.social and Laura talk about their work in #FragileNucleosome seminar series!
In case you haven't joined any of the previous 2025 sessions, here is registration link:
us06web.zoom.us/webinar/regi...

Conservation of regulatory elements with highly diverged sequences across large evolutionary distances Nature Genetics - Combining functional genomic data from mouse and chicken with a synteny-based strategy identifies positionally conserved cis-regulatory elements in the absence of direct sequence...

How to find Evolutionary Conserved Enhancers in 2025? 🐣-🐭
Check out our paper - fresh off the press!!!
We find widespread functional conservation of enhancers in absence of sequence homology
Including: a bioinformatic tool to map sequence-diverged enhancers!
rdcu.be/enVDN
github.com/tobiaszehnde...

🚨 New preprint out! Do you think Single Molecule Footprinting and Fiber-seq are super cool but aren't sure how to unlock their full potential? HiddenFoot can help you: www.biorxiv.org/content/10.1...

Join us on Wednesday next week for two exciting talks on transcription regulation from @kasitc.bsky.social and @davidsuter.bsky.social!

You can register at: us06web.zoom.us/webinar/regi...

5/2025 Issue ➡️ www.embopress.org/toc/17444292...
tissue-specific huntingtin interactomes, gene product diversity evolution
Cover: mammalian promoters characterised by low RNA pol II occupancy and high turnover @kasitc.bsky.social @molinalab.bsky.social @arnaudkr.bsky.social

New research by @kasitc.bsky.social @molinalab.bsky.social @arnaudkr.bsky.social reveals limited RNA polymerase II pausing at mouse promoters compared to flies ➡️ www.embopress.org/doi/full/10....

Pol II Pausing on LSD on the MSB cover! Credits to the amazing creative team @kasitc.bsky.social (right) and Roos (left)! Pleasure to work with this bunch of talented people, here at our Belgian retreat. Paper: doi.org/10.1038/s443...

Don't forget to checkout our annex pre-print bit.ly/3EkRqJh for a sense on our new 💻 tool FootprintCharter, for the unsupervised footprint quantification from single molecule data. Available on Bioconductor at bit.ly/3XLe8RC.

Having a stressful day? Lay back, crack a beer open and read about how chromatin accessibility responds to perturbations of TF binding at the single molecule level bit.ly/3XQMFxN. I'm incredibly thankful to my PhD mentors @arnaudkr.bsky.social and Judith Zaugg for these super productive years!!

Happy to share the latest story from @arnaudkr.bsky.social's lab @embl.org! With @guidobarzaghi.bsky.social, we used Single Molecule Footprinting to quantify how often chromatin is accessible at enhancers after TF and chromatin environment changes! Check our preprint bit.ly/3XQMFxN + thread ⬇️ 1/11

Thanks! It's less pausing in mouse. We found a higher Pol II turnover in mouse. Also the pausing index is lower in mouse.

Excited to see this out!
Using single-molecule footprinting, we showed that Pol II occupancy is lower at mouse than fly promoters. This low Pol II occupancy is explained by high Pol II turnover and low transcription initiation at mouse promoters.

Terrifying 😭😭