Juliette Bellengier

Hi.

Books are incredibly important.

Please let them be.

The evolution of antibiotic resistance in Europe, 1998–2019 Author summary Antibiotic resistance is an important public health threat: resistant infections are currently associated with 5 million deaths per year globally. This burden may increase further in th...

New paper: long-term trends in antibiotic resistance show signs of stabilisation (thread). journals.plos.org/plospathogen...

Introducing openRxiv: a new independent home for preprint sharing in the life sciences - openRxiv We’re thrilled to announce the launch of openRxiv as an independent nonprofit to oversee bioRxiv and medRxiv, the world’s leading preprint servers for life and health sciences. openRxiv ensures that r...

🎉 bioRxiv & medRxiv are now managed by openRxiv, a new independent nonprofit. This transition allows for more flexibility and innovation. Our mission remains being a free service to authors ensuring rapid sharing of scientific information before peer review.
ℹ️ openrxiv.org/introducing-...

Suite de la journée #StandUpForScienceFrance à Paris avec la table ronde à Jussieu. À dérouler ⬇️

📢 Stand Up for Science : Université Paris Cité engagée !

Ce 7 mars, nous nous associons à la mobilisation mondiale #StandUpforScience pour défendre une recherche libre et essentielle à nos sociétés.

➡️ u-paris.fr/luniversite-...

#Communiqué 🗞️ Le CNRS exprime sa solidarité avec les scientifiques exerçant aux États-Unis, où la liberté académique est aujourd'hui mise à l’épreuve.

👉 www.cnrs.fr/fr/presse/le...

L’Inserm est très concerné par le sort de la science et des scientifiques aux Etats-Unis, ayant des relations étroites historiques avec de nombreuses institutions scientifiques américaines et en particulier le NIH et des programmes scientifiques communs. Plus largement l’Inserm est solidaire du mouvement Stand Up For Science et est solidaire de tous les scientifiques exerçant aux États-Unis, où la liberté académique est aujourd’hui mise à l’épreuve. L’Institut souhaite rappeler l’importance de la science et du progrès dans le monde actuel, où nous sommes confrontés à de nombreux enjeux de société et de nombreuses crises.

L’Inserm est solidaire du mouvement Stand Up For Science

presse.inserm.fr/cest-dans-la...

#StandUpForScience2025

Welcome to the Bluesky account for Stand Up for Science 2025!

Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide!

#scienceforall #sciencenotsilence

High-Throughput Conjugation Reveals Strain Specific Recombination Patterns Enabling Precise Trait Mapping in Escherichia coli Genetic exchange is a cornerstone of evolutionary biology and genomics, driving adaptation and enabling the identification of genetic determinants underlying phenotypic traits. In Escherichia coli, horizontal gene transfer via conjugation and transduction not only promotes diversification and adaptation but has also been instrumental in mapping genetic traits. However, the dynamics and variability of bacterial recombination remain poorly understood, particularly concerning the patterns of recombined DNA fragments. To elucidate these patterns and simultaneously develop a tool for trait mapping, we designed a high-throughput conjugation method to generate recombinant libraries. Recombination profiles were inferred through whole-genome sequencing of individual clones and populations after selection of a marker from the donor strain in the recipient. This analysis revealed an extraordinary range of recombined fragment sizes, spanning less than ten kilobases to over a megabase—a pattern that varied across the three tested strains. Mathematical modelling indicated that this diversity in recombined fragment size enables precise identification of selected loci following genetic crosses. Consistently, population sequencing pinpointed a selected marker at kilobase-scale accuracy, offering a robust tool for identifying subtle genetic determinants that could include point mutations in core genes. These findings challenge the conventional view that conjugation always transfers large fragments, suggesting that even short recombined segments, traditionally attributed to transduction, may originate from conjugation. ### Competing Interest Statement The authors have declared no competing interest.

www.biorxiv.org/content/10.1...

From sequencing results' analysis, we showed that 1) our method was a valuable tool for precise trait mapping - our locus under selection could be detected with ~ kilo base precision and 2) exposed a surprisingly diversity in recombined fragment sizes contrary to precedent data on conjugation. 4/4

We successfully created libraries of donors and mixed them with several recipient bacteria (from diverse strains and phylogroups). We obtained libraries of recombinants that we analyzed at the individual and population level through sequencing 🧵 3/4

In this paper, we developed a method for the construction of donor libraries combining the power of transposon mutagenesis & lambda-red Cas9 mediated recombination🧵 2/4

I'm happy to share my first co-author preprint on the development of a system for high throughput conjugation in E. coli. Here's a quick recap of the main point: 🧵 1/4

Image décorative.

L’Inserm rejoint Bluesky !

Découvrez ici toutes les actualités de la recherche médicale menée par l’Inserm, pour la santé de toutes et tous.

Mom burrowing owl hanging out with three of her kids. Two kids have tilted heads and one kid has his eyes closed and mouth wide open.

Good morning! 🪶

ORBIT for E. coli: kilobase-scale oligonucleotide recombineering at high throughput and high efficiency Abstract. Microbiology and synthetic biology depend on reverse genetic approaches to manipulate bacterial genomes; however, existing methods require molecu

Nice article presenting a new method in E. coli for high throughput bacterial recombineering ! (new toolkit for the creation of mutant libraries 🔍)
academic.oup.com/nar/article/...

Dissecting pOXA-48 fitness effects in clinical enterobacteria using plasmid-wide CRISPRi screens Conjugative plasmids are the main vehicle for the spread of antimicrobial resistance (AMR) genes in clinical bacteria. AMR plasmids allow bacteria to survive antibiotic treatments, but they also produ...

🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14

I just made a starter pack on Ecology and Evolution of AMR.

🦠 ⚡ 💊

Feel free to suggest names and I’ll add them as soon as I can!!

go.bsky.app/CwEzc79

Chromosomal capture of beneficial genes drives plasmids towards ecological redundancy Plasmids are a ubiquitous feature of bacterial genomes, but the evolutionary forces driving genes to become associated with plasmids are poorly understood. To address this problem, we compared the fit...

New pre-print!!! What types of genes end up on plasmids and why? The take home message of this paper is that beneficial genes move from plasmids to chromosome, causing the ecological value of plasmids to decay over time.
www.biorxiv.org/content/10.1...

Announcing the new bioRxiv dashboard widget - it's now easier to when articles have comments, peer reviews, community discussion, media coverage, videos, and automated analyses 1/2 connect.biorxiv.org/news/2025/01...

Institut Pasteur decides to leave X The Institut Pasteur, a research organization which for more than 130 years has been committed to tackling infectious diseases, sharing knowledge and defending science, has decided to leave X because ...

The Institut Pasteur has decided to leave X (formerly Twitter) to join BlueSky! 🦋
The Institut Pasteur made this decision due to several serious issues observed on the X platform since its acquisition. Join us here to continue advocating for science. www.pasteur.fr/en/home/pres...

Welcome to the official Bluesky page of the Institut Pasteur!

🔬 Explore with us the frontiers of biomedical research

🧪 Follow us to uncover groundbreaking discoveries

💡 Share knowledge for global health

🌍 Join a community passionate about progress and open science.

🚨New review🚨

Evolution and ecology of anti-defence systems in phages and plasmids

Link: www.cell.com/current-biol...

Credit: Daniela Velasco Lozano/EMBL

(1/3) Antibiotic resistance is costing human lives. 🦠
A study from EMBL's Typas group proposed a framework to identify antibiotic pairs that make bacteria resistant to one antibiotic but sensitive to other – this can help design new treatments and delay resistance. 🧬🧪

www.nature.com/articles/s41...