Alexander Darlington

Modelling dynamic host-pathway interactions at the genome scale with machine learning
doi.org/10.1016/j.ym...

When talk to colleagues, I see a growing interest in the central question of this paper: what if we’ve been designing genetic circuits the wrong way? After all, natural networks *look* very different. This work, which has gone somewhat unnoticed, is one of my favourites! doi.org/10.1162/isal...

Comprehensive evaluation of the capacities of microbial cell factories - Nature Communications Constructing an efficient microbial cell factory still requires exploring and selecting various host strains, as well as identifying the best-suited metabolic engineering strategies, which demand sign...

Pseudomonas putida joins the Olympus of effective Cell Factories www.nature.com/articles/s41... Not any longer *a promising platform* for metabolic engineering, but a phenomenal, consolidated chassis for programming degradation and synthesis of amazing molecules 😃!

Great start of the year meal with the lab!

We show that optimising the design at the single cell level might not translate well when whole culture dynamics are taken into account! But don't worry we can take advantage of the host's biosynthetic constraints to simplify the design of circuit!

Design principles for engineering bacteria to maximise chemical production from batch cultures - Nature Communications Engineering microbial cell factories for chemical manufacture remains challenging due to trade-offs between production and growth. Here, the authors uncover how to design genetic circuits in...

Are you looking looking to engineer chemical production in batch cultures? In our recent paper with
Ahmad Mannan we show show to design genetic circuits to enhance productivity!
Link: www.nature.com/articles/s41...

Really happy to contribute to this fantastic work led by Harman Mehta in collaboration with Rodrigo Ledesma and
@gbstan.bsky.social!! Harman demonstrates that a microbial biosynthetic pathway can be more efficient if divided between two strains 👏🎉🎊 www.biorxiv.org/content/10.1...

At P63, Dan is showcasing his work designing feedback controllers to maximise long term performance in the face of evolution! #sbuk2024 #BiochemEvent

At P34, Mohammad can discuss our progress towards building multiscale metabolism/gene expression/growth design frameworks of yeast for #hostaware #engbio #BiochemEvent #sbuk2024

At P24, Viktoriia will present the initial whole cell modelling work we are doing to optimise recombinant membrane protein production strategies (a joint project with Doug Browning and
@alangoddard.bsky.social) #BiochemEvent #sbuk2024

Are you heading to Synthetic Biology UK? #BiochemEvent #SBUK2024 Unfortunately I am teaching but you can check out three posters from the group! Go and say hi!

Alan Goddard on LinkedIn: #biotechnology #biotech #phd #aston #industry #microbes #membranes 4 PhDs available in my lab! 1. Midlands Integrative Biosciences Training Partnership https://lnkd.in/etB6FMqC 2. Aston Institute for Membrane…

4 PhD positions available in my lab. All membranes / biotech focussed with interdisciplinary friends and industry.

www.linkedin.com/posts/alan-g...

Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media Deletion of the flagella regulator gene (fleQ) results in more consistent heterologous gene expression without significantly affecting bacterial growth, potentially due to better resource allocation....

And withe the working link dx.doi.org/10.1111/1751...

What happens if you delete the resource-expensive flagella? Published today doi.org/10.1111/1751-7…, Juhyun Kim’s group shows such deletions can create a flexible P putida cell factory!

Risk-averse optimization of genetic circuits under uncertainty
doi.org/10.1101/2024...