www.protocols.io

Promotional graphic for Springer Nature and protocols.io featuring an event announcement. The left side has a light blue background with the Springer Nature logo at the top and text reading: “Join experts for interactive sessions. Jan 21: protocols.io Hackathon. Jan 22: Methods Mythbusting Panel.” A dark blue button below says “Register today.” The right side shows the protocols.io logo and a large text design that says “I ♥ Methods” with a red heart symbol, followed by “Love Methods Week” and the hashtag #LoveMethods26.

💡 Did you know? Methods are among the most cited research outputs.
For #LoveMethods26, join experts for interactive sessions:
👉 Jan 21: @protocolsio.bsky.social Hackathon
👉 Jan 22: Methods Mythbusting Panel
spklr.io/633288eAVM

#OpenScience

We will import and format your protocol Paper protocols floating on your benchtops? We can digitize them for you!

Happy International Love Methods week!

The Crick partners with @protocolsio.bsky.social to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!)

www.protocols.io/we-enter-pro...

Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

💻Transform your protocols at our Hackathon on Jan 21

🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22

👉FREE registration & more details:

Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

💻Transform your protocols at our Hackathon on Jan 21

🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22

👉FREE registration & more details:

Graphic representing our monthly New Resource promotion featuring the name of the Library and this months chosen product - Protocols.io

Our promoted new resource for January is Protocols.io. Using Protocols.io researchers can create and share detailed experimental protocols with colleagues or the wider scientific community

www.protocols.io

Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs

at Love Methods Week 2026excelscior.uc.pt/love-methods...6/

#LoveMethods26

🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1

🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔

Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!!
www.protocols.io/view/structu...

Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost.

🌟 Full Springer Nature press release: group.springernature.com/gp/group/med...

#OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio

The image promotes a new collaboration empowering researchers to share methods, improve reproducibility, and drive global scientific progress. It includes a close-up of a computer chip with a brain illustration and the Springer Nature logo.

Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social.

Learn more: group.springernature.com/gp/group/med...

#Research 🧪

The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub) ...

Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!

We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...

YouTube video by LIBRE hub Construyendo protocolos científicos reproducibles y abiertos con Protocols.io

It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...

With the full CRISPR 7-11 protocol here :)

Graphic with a dark teal background featuring three illustrated circular icons representing different research teams. On the left, Team Kordower icon shows colorful antibody shapes labeled “Neuro-Immune Interactions.” In the center, Team Liddle icon depicts a stylized brain with interconnected circuitry labeled “Circuitry and Brain-Body Interactions.” On the right, Team Rio icon shows a human head outline with a DNA strand inside, labeled “PD Functional Genomics.” A thin gradient line runs along the bottom, shifting from purple to blue to green, with the ASAP logo in the lower right corner.

👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio!

Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉

➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)

Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...

Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:

www.medrxiv.org/content/10.1...

✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage

(1/3)

‘REF needs dedicated section for research methods’ - Research Professional News Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework

Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF:
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.

The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management This protocol standardizes the classroom administration, scoring, and data management of the Spatial Thinking Ability Test (STAT-24), a category-balanced instrument for secondar...

3. The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management
www.protocols.io/view/the-spa...

Water and sediment sampling in the sea to discover marine bacteria This protocol aims to show the strategy for obtaining water and sediment samples on board an oceanographic vessel at sea. The samples obtained can be used to identify marine bac...

2. Water and sediment sampling in the sea to discover marine bacteria
www.protocols.io/view/water-a...

Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python This protocol outlines a comprehensive workflow for intracellular metabolite analysis from microbial cultures. It begins with the rapid quenching and extraction of metabolites u...

1. Intracellular Metabolite Extraction, Peak Detection with El-MAVEN and Quantification Using Python V.2
www.protocols.io/view/intrace...

Bring structure to your research - protocols.io A secure platform for developing and sharing reproducible methods.

Explore our new featured protocols protocols.io/welcome

Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io Research is a global endeavour, yet the expectation to communicate in English often creates barriers and an additional burden for non-native speakers. At the lab bench or in t...

I've been dreaming about this for a decade and it's now live!

"Global Collaboration Made Easier: Multilingual Protocols Now Available on protocols.io"
www.protocols.io/blog/global-...

Plate CUT&Tag (Meers Lab version) This protocol describes a method for carrying out CUT&Tag-Direct in 96 well microplates for high-throughput processing of samples by a single operator with only standard lab...

We published a detailed protocol for our Plate-CUT&Tag method on @protocolsio.bsky.social to accompany our recent preprint (www.biorxiv.org/content/10.1...) give it a try! Feedback welcome! dx.doi.org/10.17504/protocols.io.n2bvjed5wgk5/v1

Copy our protocols!

📝 Find many of our lab protocols and techniques on @protocolsio.bsky.social at www.protocols.io/search?q=%22...

🎥 Go deeper with recorded video tutorials of some of our most requested methods: alleninstitute.org/education/sc...

#OpenScienceWeek

Image of the job posting text as it appears on LinkedIn 🌟 We’re Hiring: Associate / Scientific Engagement Manager 📍 Location: New York or Jersey City (Hybrid) I'm excited to share that we're looking for an Associate Scientific Engagement Manager to join the protocols.io team at Springer Nature This is a fantastic opportunity for someone to lead outreach and engagement efforts across the global research community—hosting webinars, collaborating with institutions, and supporting researchers in adopting protocols.io to improve reproducibility and accelerate discovery. 🔬 If you have a background in research, love science communication, and thrive in collaborative environments, we’d love to hear from you!

We're hiring at protocols.io (@protocolsio.bsky.social) in NY or Jersey City - Associate Scientific Engagement Manager

See LinkedIn post for more info & feel free to message me: www.linkedin.com/posts/eganle...

Network for Advanced NMR Materials SSNMR Data Acquisition Protocols The NAN Materials SSNMR Data Acquisition Protocols collection is part of the NAN Materials SSNMR Knowledgebase; a collection of resources, training and educational material, pul...

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

Bring structure to your research - protocols.io A secure platform for developing and sharing reproducible methods.

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

Re-using pipette tips for gel loading Gel loading tips can be reused for most applications; there’s REALLY no need to throw them away after a single use. In our labs, we routinely reuse tips when loading gels, onl...

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org+@nature.com papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...