www.protocols.io

Network for Advanced NMR Materials SSNMR Data Acquisition Protocols The NAN Materials SSNMR Data Acquisition Protocols collection is part of the NAN Materials SSNMR Knowledgebase; a collection of resources, training and educational material, pul...

Protocols @nmrfam.bsky.social Alex Paterson, Network for Advanced NMR Materials (NAN) Solid-State NMR Data Acquisition Protocols V.2 www.protocols.io/view/network... #NMRchat #NMR 🧲

Bring structure to your research - protocols.io A secure platform for developing and sharing reproducible methods.

Author here to answer any questions. The full protocol is online at protocols.io and all codebooks on github

Re-using pipette tips for gel loading Gel loading tips can be reused for most applications; there’s REALLY no need to throw them away after a single use. In our labs, we routinely reuse tips when loading gels, onl...

Load gels? Use #zebrafish 🐟? Want to do more sustainable research w/out compromising science? We-G Gestri, K Turner, G Powell @stevewilsonlab.bsky.social-made these protocols. Yes, we use these in our @science.org+@nature.com papers.
www.protocols.io/view/re-usin...
www.protocols.io/view/washing...

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Bas... Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the tru...

Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation www.protocols.io/view/compara...

CRAFTseq Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification of the causal alleles and their functions remain critical bottl...

Happy to answer any and all questions about this work. We'd love to see more people try it.
Full protocol: www.protocols.io/view/craftse...
Full analysis: github.com/immunogenomi...

Precisely defining disease variant effects in CRISPR-edited single cells - Nature A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...

I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.

www.nature.com/articles/s41...

Thank you for sharing your methods knowledge publicly!

The easiest publishing process ever! After testing & retesting this protocol adapted from a large-volume industry standard method, I finally clicked “Publish” over at @protocolsio.bsky.social today. Can recommend; would publish again!! 🌾 🍞 🧪

dx.doi.org/10.17504/pro...

🚨🚨 We just published a protocol for non-lethally measuring morphology in 3D in small aquatic organisms!! 🚨🚨 www.protocols.io/view/non-let...

The work was led by undergrad Daniela Granados 🎉🎉 and our collaborators include Najva Akbari, and Lauren O'Connell!

Last month we published our protocol for detection of beet yellows virus. Here it is!

RT-LAMP for BYV detection www.protocols.io/view/rt-lamp...

Identification of Corynebacteria of the diphtheriae Species Complex and detection of the diphtheria toxin g... This protocol describes a quadruplex real-time PCR (Dip4plex), performed with the Qiagen QuantiNova™ Multiplex PCR Kit, for simultaneous identification of Corynebacteria of th...

qPCR diagnosis of #diphtheria: you may be interested by our procedure, which is now widely used across European reference laboratories: www.protocols.io/view/identif... @EURLdiphtheriaPertussis thanks @chcrestani.bsky.social for releasing on protocols.io

Happy to be in Brno for the #CTLS2025 conference from the Core Technologies for Life Sciences society. I'll be talking about protocols.io tomorrow morning in the Core Facilities and Open Science session

YouTube video by protocolsio Protocol Spotlight with Maria Diehl

Spotlight interview with Maria Diehl regarding her PLOS ONE Lab Protocol paper: "Modifying the platform-mediated avoidance task: A new protocol to study active avoidance within a social context in rats." @realdiehllab.bsky.social

What your Methods section isn’t If you’re a scientist like me, you read a lot of papers – and a lot of submitted manuscripts, drafts, and other larval stages of papers.* This is extremely helpful when it’s time to write your own …

For scientific writers: Three things your Methods section shouldn't be - but three things Methods sections often end up being. You can avoid these mistakes! scientistseessquirre...

A fantastic start to #OSFest25 this morning, looking at publishing methods and protocols with @protocolsio.bsky.social. With three different sessions tomorrow and more over the week ahead, it’s not too late to have a look at the festival schedule and register your place: buff.ly/jZsSd6U

This month, we are recognizing the Assessment of Motor and Non-Motor PD Symptoms Working Group as our Network Spotlight! They shared protocols to support more reproducible rodent behavioral assays in #ParkinsonsDisease research 🐁 🧠

🔗 Check them out: www.protocols.io/view/behavio...

Today. bsky.app/profile/ltey...

🥼 Do you want to start working with human pluripotent stem cells?

📖 Check out our protocols collection "Good practices in hPSC handling", developed among the CorEuStem COST Action Project at COST Association

#stemcells

www.protocols.io/view/good-pr...

Talking about training and opportunities for technicians around publishing. We're trying to get ours interested in using protocols.io to share work they're already doing optimising protocols.
#DARTS9

Why so detailed? I did Hydra care as a postdoc and when I tried to set up our lab Hydra it went horribly! After joining our lab, Kelso looked through multiple protocols and did some troubleshooting until our lab populations were thriving.

Interested in studying Hydra? Our lab researcher Kelso Cochran wrote a comprehensive protocol (used to train all of our undergrads) now shared on protocols io on how to get started www.protocols.io/view/compreh...

Protocol Exchange transitioned to protocols.io! When protocols.io was acquired by Springer Nature two years ago, we wrote that it would result in an expansion and improvement of our platform, without compromising our business...

The protocols from Protocol Exchange are now live on protocols.io, expanding the @protocolsio.bsky.social repository from 21,000 to 23,000 public protocols. www.protocols.io/blog/protoco...

YouTube video by protocolsio Protocol Spotlight with Fotios Alexandros Karakostis

Spotlight interview with Fotios Alexandros Karakostis regarding his PLOS ONE Lab Protocol paper: "Introducing “Validated entheses-Based reconstruction of activity 2.0” (VERA 2.0): Semi-automated 3D analysis of bone surface changes."

Open Science & Scholarship Festival 2025 now open for booking! | UCL Open@UCL Blog UCL Homepage

You've heard about the upcoming festival, but let's take a look at some of the individual sessions!

The first event on the programme is taking place in person at @crick.ac.uk and will be featuring experts from @protocolsio.bsky.social. The workshop is open to all.

YouTube video by protocolsio Protocol Spotlight with Daniel Foulkes

Spotlight interview with Daniel Foulkes regarding his PLOS ONE Lab Protocol paper: "Stable topical application of antimicrobials using plumbing rings in an ex vivo porcine corneal infection model."

Efficiently constructing complete genomes with CycloneSEQ to fill gaps in bacterial draft assemblies Efficiently constructing complete genomes with CycloneSEQ to fill gaps in bacterial draft assemblies

And the paper: gigabytejournal.com/articles/154

Bring structure to your research - protocols.io A secure platform for developing and sharing reproducible methods.

When publishing the first benchmarking data for a new sequencing technology like CycloneSeq, it's great having @protocolsio.bsky.social to share the protocols in a transparent manner.

CycloneSEQ Library Construction and Sequencing Protocol for Isolated Bacteria. dx.doi.org/10.17504/pro...

Are you a researcher who'd like to know more about creating open methods & protocols? Can you be in London 2nd June am?

Then come to the training event that the Crick & protocols.io are putting on for the Open Science & Scholarship Festival.

Register here: forms.office.com/e/EecPjyU1UU

YouTube video by protocolsio Protocol Spotlight with Elisa Crisci

Spotlight interview with Elisa Crisci regarding her PLOS ONE Lab Protocol paper: "High-throughput 96-well plate-based porcine antibody isolation protocol."

Entorhinal cortex represents task-relevant remote locations independent of CA1 Neurons can collectively represent the current sensory experience while an animal is exploring its environment or remote experiences while the animal is immobile. These remote representations can refl...

I'm late to the party, but here's a 🦋 intro!

I'm a postdoc at Stanford studying how the hippocampal circuit supports multiple aspects of spatial memory and how it goes awry in aging and #Alzheimers. Starting my own lab soon 👀

👉 www.biorxiv.org/content/10.1...
👉 www.protocols.io/view/chronic...