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Nat Prunet

@nat-prunet.bsky.social

Microscopic farmer πŸ”¬πŸŒΊ Microscopy core director @UNC Chapel Hill Former plant developmental biologist

1,243 Followers  |  1,268 Following  |  163 Posts  |  Joined: 14.11.2024  |  1.9447

Latest posts by nat-prunet.bsky.social on Bluesky

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Going to Cell Bio 2025 in Philly? Join us at the Science and Art session on Sunday! You may know that I love the intersection of art and science. I'll be talking about the art of microscopy πŸ”¬πŸ‘¨β€πŸŽ¨

#sciart #bioart #cellbio #cellfie #microscopy #scicomm

04.12.2025 14:49 β€” πŸ‘ 10    πŸ” 2    πŸ’¬ 0    πŸ“Œ 1
On the cover of New Phytologist volume 249, issue 1: Confocal microscopy image showing DR5:3nucVenus expression (yellow) in the inflorescence apical meristem (cyan) of an Arabidopsis thaliana plt3 plt5 plt7 pin1-T600I mutant. The DR5:3nucVenus auxin-responsive reporter highlights regions of auxin accumulation and signalling activity within the mutant inflorescence apical meristem. Image courtesy of Merijn Kerstens and Viola Willemsen.

On the cover of New Phytologist volume 249, issue 1: Confocal microscopy image showing DR5:3nucVenus expression (yellow) in the inflorescence apical meristem (cyan) of an Arabidopsis thaliana plt3 plt5 plt7 pin1-T600I mutant. The DR5:3nucVenus auxin-responsive reporter highlights regions of auxin accumulation and signalling activity within the mutant inflorescence apical meristem. Image courtesy of Merijn Kerstens and Viola Willemsen.

#LatestIssue cover: Confocal #microscopy image showing DR5:3nucVenus expression in the inflorescence apical meristem of an Arabidopsis thaliana plt3 plt5 plt7 pin1-T600I mutant. Image courtesy of Kerstens and Willemsen.

πŸ“– See @merijnkerstens.bsky.social & Willemsen et al.
doi.org/10.1111/nph....

04.12.2025 13:03 β€” πŸ‘ 11    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0
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Next on the 2026 roster is Dr. Peter Kner, @knerlab.bsky.social. He lectures on wave #optics, #diffraction, and #contrast generation in imaging. This Physics 101 gets everyone on the same #wavelength, even if your background is all biology. Apply now to join us! bit.ly/49YQl7q

#microscopy

22.11.2025 14:15 β€” πŸ‘ 6    πŸ” 2    πŸ’¬ 0    πŸ“Œ 1
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On the second day of AQLM, we also have Dr. Steve Ross of #Nikon Instruments who gives us an inside look at #optics, objective #lenses, and #image aberrations. You've (hopefully) never seen an objective like this before!

Apply now to join us April 29th-May 8th 2026. bit.ly/49YQl7q
#microscopy

24.11.2025 23:01 β€” πŸ‘ 10    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
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Day 2 also covers Fourier Optics, with a fantastic lecture from Dr. Peter Kner! You'll never understand Fourier transforms better than you do after Peter explains it, trust us.

Apply now to join us and finally understand #Fourier space! bit.ly/49YQl7q

#microscopy

28.11.2025 20:40 β€” πŸ‘ 8    πŸ” 2    πŸ’¬ 0    πŸ“Œ 1
Actin and vimentin in a COS7 cell.

Actin and vimentin in a COS7 cell.

Mitochondria in a COS7 cell.

Mitochondria in a COS7 cell.

Actin and vimentin in COS7 cells.

Actin and vimentin in COS7 cells.

Our M.Sc. PBioC (Physical Biology of Cells and Cell Interactions) students at @goetheuni.bsky.social had their first hands-on encounter with STED microscopy this week β€” and they captured some truly stunning super-resolution images.

#FluorescenceFriday #Superresolution

28.11.2025 23:58 β€” πŸ‘ 22    πŸ” 3    πŸ’¬ 0    πŸ“Œ 0
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All #CellBio2025 attendees! Here’s our phenomenal lineup for our session on #SciArt & #SciComm: @ahnaskop.bsky.social, @jiwasa.bsky.social, @mag2art.bsky.social, @nat-prunet.bsky.social, and Julia Licholai. Please come by and spread the word! Huge thanks to @ascbiology.bsky.social and co-organizers!

01.12.2025 17:11 β€” πŸ‘ 25    πŸ” 15    πŸ’¬ 2    πŸ“Œ 2

I’m sure you will!

01.12.2025 16:09 β€” πŸ‘ 1    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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Love the #SciArt gallery on @maikbischoff.bsky.social's lab website 😍

www.bischofflab.com/portfolio

#microscopy #bioart #microscopymonday #cellfie #devbio #cellbio

01.12.2025 15:32 β€” πŸ‘ 21    πŸ” 3    πŸ’¬ 2    πŸ“Œ 1
The brand new website of the Bischoff lab

The brand new website of the Bischoff lab

... together with my SciArt Gallery :)

... together with my SciArt Gallery :)

So excited to share this as a new junior PI:

My brand-new lab website! πŸŽ‰πŸͺ°πŸŒ€
www.bischofflab.com

Please pass it on to young, motivated researchers looking for PhD positions 😊

And for the #FluorescenceFriday community: don’t miss the SciArt Gallery!

#CellBio #DevBio #PhDjob #PhDposition #Science

27.11.2025 08:26 β€” πŸ‘ 165    πŸ” 50    πŸ’¬ 12    πŸ“Œ 4
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51st #NikonSmallWorld winners reveal a hidden world of wonders: zorz.it/PqBOh

#JeremyGray #51stAnnualNikonSmallWorldPhotomicrographyCompetition #macro #winners #micro #microscopy #Nikon #PhotoContest #PhotoMicrography

28.11.2025 23:20 β€” πŸ‘ 2    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
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Studying cochlear neuroanatomy demands imaging the entire cochlea at subcellular resolution. In our recent study (tinyurl.com/isotropicLSFM), we recorded the cochlea at isotropic resolution across the whole organ, laying the groundwork for what follows…

#lightsheet
#isotropic
#cochlea

20.11.2025 21:20 β€” πŸ‘ 19    πŸ” 5    πŸ’¬ 1    πŸ“Œ 0
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Important video from fellow scientist and microscopist @stephanie-shiers.bsky.social. Please respect our work.
#Microscopy #MicroscopyMonday

24.11.2025 08:53 β€” πŸ‘ 74    πŸ” 24    πŸ’¬ 1    πŸ“Œ 1

Love that cuttlefish bottle 😍

20.11.2025 16:08 β€” πŸ‘ 5    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
Preview
HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton - preLights HAK-actin is a new probe that may help researchers see the actin cytoskeleton in sharper detail using expansion microscopy.

Reliable actin labelling in expansion #microscopy πŸ”¬

Beautiful #preprint from Olivier Mercey, @lreymond.bsky.social & team @dudinlab.bsky.social @centriolelab.bsky.social

Read the latest #preLight from @kanishka03.bsky.social β¬‡οΈπŸ‘€
prelights.biologists.com/highlights/h...

19.11.2025 11:57 β€” πŸ‘ 46    πŸ” 13    πŸ’¬ 0    πŸ“Œ 3
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First up is AQLM Director, Dr. Alexa Mattheyses. @mattheyseslab.bsky.social She kicks off the course with an introduction to #optics, where you build a #Kohler #microscope using nothing but #lenses and #physics.

If this sounds like your jam, apply now to join! bit.ly/49YQl7q
#microscopy

20.11.2025 13:20 β€” πŸ‘ 5    πŸ” 2    πŸ’¬ 0    πŸ“Œ 1
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I’m proud to present the main work from my postdoc, 3+ years in making from scratch, my ultimate #MimulusPropaganda up till now:

The Moment
That
Symmetry
Breaks.

#plantscience #development #imaging #microscopy πŸ§ͺπŸŒΈπŸ”¬
w. Captain Yaowu & @biancatash.bsky.social

www.biorxiv.org/content/10.1...

07.08.2025 14:44 β€” πŸ‘ 226    πŸ” 65    πŸ’¬ 13    πŸ“Œ 7
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More extraordinary model systems for regeneration ABSTRACT. The ability of organisms to replace and regenerate anatomical structures following their loss or damage has piqued the curiosity of biologists for centuries. In addition to Development's β€˜Mo...

Excited to share this perspective article out now! Come read about Streptocarpus and other great model systems!

More extraordinary model systems for regeneration journals.biologists.com/dev/article/...

28.10.2025 13:23 β€” πŸ‘ 15    πŸ” 6    πŸ’¬ 1    πŸ“Œ 1
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Applications are now open for AQLM 2026! Join us @mblscience.bsky.social April 29th-May 8th, 2026 for a comprehensive, hands-on light microscopy course with an amazing roster of faculty who are leading experts in their fields!

07.11.2025 14:29 β€” πŸ‘ 4    πŸ” 3    πŸ’¬ 1    πŸ“Œ 0
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Key transcripts encoded centrin-like proteins, a frequent component of sensory/contractile structures in protists. We visualized tentacle ultrastructure by U-ExM with @dudinlab.bsky.social lab, discovering stunning tip and collar structures 🀯that add structural complexity to new tentacle formation.

18.11.2025 16:15 β€” πŸ‘ 24    πŸ” 8    πŸ’¬ 1    πŸ“Œ 1
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Cellular structure self-organizes through an interplay between internal mechanisms and external cues. The single-celled suctorian P. collini builds a trap structure to capture large prey using microtubule feeding tentacles, creating feedback between cell morphology and prey availability.

18.11.2025 16:15 β€” πŸ‘ 90    πŸ” 22    πŸ’¬ 2    πŸ“Œ 14

A student-run radio show covered my Nikon Small World honorable mention & I even got to give a radio interview!
A totally new experience for me πŸ˜€

Huge thanks to Andrew!

Also featuring @peiferlabunc.bsky.social & @nat-prunet.bsky.social

#NikonSmallWorld #Microscopy #ScienceCommunication #SciArt

19.11.2025 11:06 β€” πŸ‘ 36    πŸ” 8    πŸ’¬ 2    πŸ“Œ 0
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Never seen the death of a ciliate like this-- usually it's too much pressure from the coverslip or too much osmotic pressure. But this gradual posterior-to-anterior "unzipping" effect amazed me !

Motic BA310e - 40x Objective - sped up 2X
#microscopy #Paramecium #Iteachbiology #edusky

17.11.2025 14:25 β€” πŸ‘ 21    πŸ” 6    πŸ’¬ 2    πŸ“Œ 1

with Arabidopsis we ensure that the female side of the cross is virgin by emasculating them. Those stamens have to go βœ‚οΈ

14.11.2025 20:41 β€” πŸ‘ 2    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
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I was today years old when I learned that *Drosophila husbandry* was actually a skillset to add to a cell/developmental biology resume. I wonder if I should add Arabidopsis husbandry to mine πŸ˜†

#drsophila #cellbio #devbio #arabidopsis #plantscience

14.11.2025 19:41 β€” πŸ‘ 13    πŸ” 1    πŸ’¬ 1    πŸ“Œ 0

If you are looking for #microscopy events, check out our Microscopy events calendar, powered by MicroscopyDB.

πŸ—“οΈ: focalplane.biologists.com/events/
Add an event: microscopydb.io/add-a-commun...

12.11.2025 14:06 β€” πŸ‘ 1    πŸ” 2    πŸ’¬ 0    πŸ“Œ 0
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Flashback #FluorescenceFriday to some of the first light-sheet imaging we were doing. Video shows the collecting duct system of the developing kidney labeled with a pan-cytokeratin antibody. Courtesy of former talented technician Deanna Hardesty.

07.11.2025 16:43 β€” πŸ‘ 77    πŸ” 22    πŸ’¬ 1    πŸ“Œ 1
JCS Cover: Human tracheal epithelial cells (hTECs) from an air–liquid interface culture imaged using ultrastructural expansion microscopy (U-ExM). Cells are labelled for tubulin (magenta), mCLING (cyan) and DNA (blue, DAPI). Image from Olivier Mercey and Marine Brunet in the Guichard-Hamel lab. See article by O. Mercey et al. (jcs264260).

JCS Cover: Human tracheal epithelial cells (hTECs) from an air–liquid interface culture imaged using ultrastructural expansion microscopy (U-ExM). Cells are labelled for tubulin (magenta), mCLING (cyan) and DNA (blue, DAPI). Image from Olivier Mercey and Marine Brunet in the Guichard-Hamel lab. See article by O. Mercey et al. (jcs264260).

Our #JCSciliaSI is complete

Explore our ToC: journals.biologists.com/jcs/issue/13...

Our cover image shows tracheal epithelial cells from an air-liquid interface culture imaged using U-ExM from Oliver Mercey and Marine Brunet @centriolelab.bsky.social.
journals.biologists.com/jcs/article/...

04.11.2025 13:35 β€” πŸ‘ 21    πŸ” 7    πŸ’¬ 1    πŸ“Œ 1
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I had to try it. It was really good!

#microscope #microscopy

06.11.2025 18:45 β€” πŸ‘ 9    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

Gorgeous cover by @mathpreu.bsky.social πŸ‘

06.11.2025 11:54 β€” πŸ‘ 6    πŸ” 2    πŸ’¬ 1    πŸ“Œ 0

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