Ross Wang Lab

Nothing better than to start the day with a Thankyou email reporting her success, from a student who was in the class we taught 3 years ago :)

Today's Bioorganic bingo 😂

Congrats 🎉

Congrats 🎉

Congratulations to our Ben Prather 🎉🎊 #chembio #chemsky

Congrats!

Check out our lab’s grad student Ben’s poster tonight #ACSSpring2025, which is supported by the ACS travel grant @acsbiol.bsky.social #chemsky #chembio

Congrats 🎉🍾

I was that woman! I have frequently told the story of how Jeremy responded after my second NIH rejection. He said, “Whatever you do, don’t stop spending. If you run out of startup, we’ll find more money for you.” I didn’t need it in the end, but knowing he had my back was huge. Thank you!

Thanks Laura!

Many thanks Roland!

Thank you Aaron! 🙏

We will see 🤣. Hope things will eventually get better on that side

Being notified today that the study section review for one recent proposal is complete. This is a resubmission from the previous attempt (impact score 45). Just to show a bit positive side that part of the NIH is still functioning and the current review system greatly helps improve our research.

Congrats 🍾

Development of mirror-image monobodies targeting the oncogenic BCR::ABL1 kinase - Nature Communications In this work, the authors develop mirror-image monobodies (Mb) made of D-amino acids against the BCR::ABL1 SH2 domain with high binding affinities. The heterochiral Mb-SH2 structures reveal an unusual...

🎄Xmas gift!🎁Joint work with #OliverHantschel in @naturecomms.bsky.social 💥! Huge kudos 💪🏼😻 Nina 👩‍🔬 for leading as 1st author & Frank 👨🏼‍🔬 for training her like a pro❤️
Small delay😅 -my👵broke her hip🤦🏼‍♀️-loved ones always come first❤️surgery went good💪🏼🤩Let's celebrate🥳🥂! #ChemBio
www.nature.com/articles/s41...

Starter pack here for protein modifications! All PTMs, enzymes, biochemistry, protein fate and function, cell signaling +++ go.bsky.app/HqEcaCw

Metabolic Probing of Sialylated Glycoconjugates with Fluorine-Selenol Displacement Reaction (FSeDR) Dysregulated sialic acid biosynthesis is characteristic of the onset and progression of human diseases including hormone-sensitive prostate cancer and breast cancer. The sialylated glycoconjugates involved in this process are therefore important targets for identification and functional studies. To date, one of the most common strategies is metabolic glycoengineering, which utilizes N-acetylmannosamine (ManNAc) analogues such as N-azidoacetylmannosamine (ManNAz) to hijack sialic acid biosynthesis and label the sialylated glycoconjugates with “click chemistry (CuAAC)” tags. Yet, current chemical modifications including those CuAAC-based alkyne/azide tags are still big in size, and the resulting steric hindrance perturbs the mannosamine and sialic acid derivatives’ recognition and metabolism by enzymes involved in biosynthetic pathways. As a result, the peracetylated ManNAz has compromised incorporation to sialic acid substrates and manifests cellular growth inhibition and cytotoxicity. Herein, we show that the α-fluorinated peracetylated analogue ManN(F-Ac) displayed a satisfying safety profile in mammalian cell lines at concentrations as high as 500 μM. More importantly, aliphatic selenol-containing probes can efficiently displace α-fluorine in fluoroacetamide-containing substrates including ManN(F-Ac) at a neutral pH range (∼7.2). The combined use of peracetylated ManN(F-Ac) and the dethiobiotin-selenol probe as the fluorine-selenol displacement reaction (FSeDR) toolkit allowed for successful metabolic labeling of sialoglycoproteins in multiple prostate and cancer cell lines, including PC-3 and MDA-MB-231. More sialoglycoproteins in these cell lines were demonstrated to be labeled by FSeDR compared with the traditional CuAAC approach. Lastly, with FSeDR-mediated metabolic labeling, we were able to probe the cellular expression level and spatial distribution of sialylated glycoconjugates during the progression of these hormone-sensitive cancer cells. Taken together, the promising results suggest the potential of the FSeDR strategy to efficiently and systematically identify and study sialic acid substrates and potentially empower metabolic engineering on a diverse set of glycosylated proteins that are vital for human diseases.

Happy to share our latest studies of using fluorine-mediated steric-free #bioorthogonal labeling to study sialic acid biosynthesis during cancer cell progression. #glycotime #chembio
#chemsky

pubs.acs.org/doi/10.1021/...

We will be thrilled if can be included. We are primarily working on protein acetylation, glycosylation, phosphorylation, and also some rare PTMs related to tyrosine

Got the chance to put together the "Click of Chemical Biologists" starter pack v1 and v2 over the Thanksgiving Break. Feel free to ping me on who else I might be missing!
V1: go.bsky.app/3P5i7nJ
V2: go.bsky.app/Cr4ej7

Thank you Mike 🙏

Thanks Mike!

Check out our latest publication at JBC, using a D chiral unnatural amino acid at the i+4 site for fluorine-displacement stapled peptides, which displayed enhanced membrane penetration and nuclear uptake than L for the estrogen receptor/coactivator interaction #chembio
www.jbc.org/article/S002...

Sensitive PFAS detection used to be long lagging

Great list! We would be thrilled to be included in if you get space :)

Test posting our 1st one here #chemsky🦋: Check out our recent article on ‘Fluorine-thiol displacement stapling on the Disordered pKID Domain to probe the CREB/CBP protein-protein interactions…’ published in Synlett. #ChemicalBiology #Peptide #Protein
Link - www.thieme-connect.com/products/ejo...