Changyang Linghu

Thank you!

Thank you! We tested it in HeLa cells, and it worked. We haven't tested it in stem cells yet. Curious to see how it works in stem cells!

Thank you!

Thank you!

This work is led by amazing lab members: Lirong Zheng, Dongqing Shi, Yixiao Yan. We thank collaborating labs: Pietro Liò, Donglai Wei, Ed Boyden, Denise Cai, Michael Roberts, Sethu Pitchiaya. Sponsors: NIH, @afar.org, @chanzuckerberg.bsky.social, @klingensteinorg.bsky.social, Glenn & Whitehall Fdns.

We found that mice expressing CytoTape in the brain retained normal cognition, learning, memory, and locomotor functions. We performed molecular, electrophysiological, and network analysis and found that CytoTape preserves normal cellular physiology in vitro and in vivo.

We applied CytoTape for scalable, spatiotemporally resolved single-cell recording in the living mouse brain, enabling recording of transcriptional activity histories across up to 14,123 neurons per mouse spanning multiple brain regions.

We demonstrated its utility across multiple mammalian cell types, achieving simultaneous recording of five transcription factor activities and gene transcriptional activities. CytoTape reveals divergent transcriptional trajectories and complex temporal modes across cells.

Because temporal information is stored as molecular tags along CytoTape, enhancing the recording capacity would require more tags along the recording axis (max length) and more kinds of possible tags (bit depth), and we increased both, building from our earlier XRI technology.

CytoTape in HEK

CytoTape records temporal cell activities across space on a thread-like elongating protein fiber in live cells, for post-fixation/post-mortem readout at scale under conventional light microscopy. It breaks through the trade-off between resolution and scale in live-cell imaging.

CytoTape in a neuron

Our CytoTape work is published today in Nature @nature.com! CytoTape is a genetically encoded, flexible, intracellular protein tape recorder for spatiotemporally scalable and multiplexed recording of cellular activities continuously across weeks in vitro and in vivo. www.nature.com/articles/s41...