Richard Eva Lab

Our latest review with @doctorpaf.bsky.social! We discuss the breadth of therapeutic potential for the neurodegenerative enzyme SARM1.

-Challenges for SARM1 inhibition for neuroprotection
-Roles in non-neuronal cells
-SARM1 activation for selective peripheral neuroablation

dlvr.it/TNnCBh

Targeting SARM1: from inhibition for neuroprotection to activation for neuroablation Sterile alpha and TIR motif-containing protein 1 (SARM1),is a central enzyme that drives programmed axon degeneration and has gathered significant interest as a therapeutic target. Despite preclinical...

In this review @Andrea-Loreto.bsky.social and I discuss the breadth of therapeutic potential for the neurodegenerative enzyme SARM1

- Challenges for SARM1 therapeutic inhibition

- SARM1 in non-neuronal cells

- SARM1 activation for selective peripheral neuroablation.

www.cell.com/trends/pharm...

I’m excited to share that my PhD work is out in @jcb.org! Big thanks to my amazing mentor @stephgupton.bsky.social and inspiring collaborator @coronin.bsky.social!!!!

Congratulations, this is excellent news!

Single-cell RNA sequencing clarifies dermal fibroblast subset representation in vitro and reveals variable persistence of keloid disease-associated features In vitro models of scarring and fibrosis are essential to improve our understanding of disease mechanisms and ultimately develop much-needed effective therapeutic strategies. This is particularly true...

Happy to announce our preprint going live on BioRxiv! Interested in what’s happening to your fibroblasts’ transcriptomic phenotype once it’s in culture? Look no further!

www.biorxiv.org/content/10.1...

@denklab.bsky.social
#fibroblast #scRNAseq #culture #invitro #keloid #ECM #matrix #mesenchymal

Deep Purple Sweet Potato

The long and newly updated story of lithium and its potential role for helping to prevent Alzheimer's disease
erictopol.substack.com/p/lithium-an...

A huge thank you to the Eva and Bradbury Lab members, @wolfsonsparc.bsky.social, and all the GRC CNS Injury and Repair attendees. So much fun, so much science and all top quality. A poster prize for Zahra to top it off! New ideas, collaborations, connections. As a great conference should be!

Love this. Who needs sheets!?

Synaptotoxic effects of extracellular tau are mediated by its microtubule-binding region - Acta Neuropathologica Immunotherapies targeting extracellular tau share the premise that interrupting cell-to-cell spread of tau pathology in Alzheimer’s disease (AD) will slow dementia pathogenesis. Whether these interven...

*New paper alert* - extracellular tau blocks synaptic plasticity in vivo via its microtubule-binding domain, which can be rescued by our Gen2 tau antibodies - all the product of a great collaboration with Michael Rowan and colleagues at @tcddublin.bsky.social link.springer.com/article/10.1...

What an interesting paper, such a huge amount of work! Great to see it cover the different CNS and PNS axon types.

And thank you for the thread: enjoyed following the story, showing how many people were involved, how long it took, and how it all came together. A phenomenal community effort!

Regenerating Retinal Neurons using AAV Vectors / ISCRM .

Researchers in the Reh Lab have produced the clearest evidence yet that AAV-delivered genes can stimulate regeneration in the mammalian retina. @tomreh.bsky.social Marina Pavlou. #retina #regeneration @uwmedicine.bsky.social

shorturl.at/qVUKz

Our paper is finally out!

Neuronal autophagy controls excitability via ryanodine receptor-mediated regulation of calcium-activated potassium channel function.
www.pnas.org/doi/abs/10.1...

Very cool! Some axonal GAPDH mRNA? Mature neurons?

A fantastic place to work with brilliant colleagues in a vibrant part of London!
Wolfson SPaRC is hiring for Lecturer and above-level roles. Come and be part of the exciting work happening here. Amazing people, meaningful impact, and a location that’s hard to beat.

Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration - Nature Communications Therapeutic interventions for motor axon regeneration following peripheral nerve injury are currently unavailable. Here authors show local administration of a non-muscle myosin II inhibitor at the inj...

Excellent story from the Woolf lab!
www.nature.com/articles/s41...

Combinatorial approaches increasing neuronal activity accelerate recovery after spinal cord injury Chen et al. report that patients with spinal cord injury who received treatment with a combination of Hebbian stimulation to strengthen corticospinal synap

Significant functional improvements in walking speed/endurance for people with spinal cord injury with combined 4-aminopyridine and paired associative stimulation.

A great study by Dr. Monica Perez and an All Star group.

#SCIrehab 🧪 #phySKYatry

academic.oup.com/brain/advanc...

Biphasic inflammation control by dedifferentiated fibroblasts enables axon regeneration after spinal cord injury in zebrafish Fibrosis and persistent inflammation are interconnected processes that inhibit axon regeneration in the mammalian central nervous system (CNS). In zebrafish, by contrast, fibroblast-derived extracellu...

Excited to share the latest work from our lab: www.biorxiv.org/content/10.1...

That's definitely a smile.

Some interesting findings for ribosomal populations in mature human neurons...

Disruption of G3BP1 granules promotes mammalian CNS and PNS axon regeneration | PNAS Depletion or inhibition of core stress granule proteins, G3BP1 in mammals and TIAR-2 in Caenorhabditis elegans, increases the growth of spontaneous...

Our CNS regeneration work has now been published in @PNAS. It was a huge undertaking in collaboration with @Twiss @Benowitz @WardLab_Emory @KristyWelshhans @Marktuszynski @jenniferDulin
@ArthurEnglish @JohnHolule labs. Thank you all, and @MerkinPnnrCtr, for funding.

www.pnas.org/doi/10.1073/...

Ooh, that's very interesting...

Thank you!

Very helpful , thank you! Room for an axon protection and regeneration lab!?

An exciting paper demonstrating a complex integrin trafficking mechanism with potential implications for the machinery regulating axon growth and regeneration. Intriguing!

FAK modulates glioblastoma stem cell energetics via regulation of glycolysis and glutamine oxidation Glycolysis and the TCA cycle are reprogrammed in cancer cells to meet bioenergetic and biosynthetic demands, including by engagement with the extracellular matrix (ECM). We show that focal adhesion ki...

Masalmeh, RHA, et al. FAK modulates glioblastoma stem cell energetics via regulation of glycolysis and glutamine oxidation. bioRxiv posted 20 February 2025 doi:10.1101/2024.12.11.627900. www.biorxiv.org/content/10.1...

Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain - Nature Methods A first-in-class fluorescence lifetime-based FRET sensor for phosphatase and tensin homolog (PTEN) enables the dynamic monitoring of PTEN activity in cultured cells and in vivo with high spatiotempora...

I started my lab more then 4 years ago, and we have endured some very challenging times ever since.…I am very excited to share the first paper from my lab, published now in @naturemethods.bsky.social
www.nature.com/articles/s41...

Gif showing a spine containing spine apparatus, reconstructed from FIB-SEM images. ER: red, synapse: yellow, plasma membrane: blue

Our paper is out just in time for #EM_Monday! It is about a specialized neuron-specific ER, called the spine apparatus, which is found close to synapses and has a very peculiar shape. If you think neurons are special, the spine apparatus is their🦄 horn! But how does it form?!
doi.org/10.1016/j.cu...

ICYMI our latest paper is now out officially in print (is there still such thing?). And since Dev Cell @cellpress.bsky.social did not select our submission for cover, I post it here. A really cool illustration from my son Zack. www.cell.com/developmenta...

Aged motor neurons display an impaired response to muscle BDNF, consistent with downregulation of TrkB at the neuromuscular junction.