Stephane Vassilopoulos

#VAMP7-dependent late endosomal #secretion of #ER and #mitochondrial proteins impacts the #tumor #microenvironment and #macrophage engagement
www.nature.com/articles/s41...

Our article on how to use micropatterned presynapses-on-glass is out in its final form in the latest issue of the Journal of Neuroscience @sfnjournals.bsky.social: www.jneurosci.org/content/46/8...
The cover went to the special section on social neuroscience, so here's our #notthecover attempt

Gotta love Nature's opinion pieces

"Science funding needs fixing - but not like that"

"Academic publishing is broken - but publishing less is not the solution"

"AI will kill science - how can researchers use it to be more efficient?"

FCHo2, not talin, enables inside-out activation of integrin ɑvβ5 in curved adhesions
www.nature.com/articles/s41...

Montage of COS7 cells stained for actin and imaged on an Apotome microscope

A menagerie of COS cells for #MicroscopyMonday 🧪

My personal obituary for Richard Hynes now live on the Manchester Cell-Matrix Centre website. www.manchester-matrix.org/news/profess...

This is finally out in J Neuroscience! A micropatterned presynapse-on-glass approach to resolve the nanoscale architecture and function of presynapses
www.jneurosci.org/content/earl...

A Modular Encapsulation System for Precision Delivery of Proteins, Nucleic Acids, and Small Molecules Targeted nanoparticles have the potential to revolutionize therapeutics for medical applications. Here, we demonstrate the utility of a flexible precision nanovesicle delivery system for functional de...

Excited to share our work engineering caveolin-derived nanoparticles “caveospheres”. We show their use for targeted transfection in cultured cells and effective targeted delivery and tumor killing in vivo.
pubs.acs.org/doi/full/10....

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...

🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9

An epidermal membrane-associated periodic skeleton restricts endocytosis to stabilize neuron-epidermal attachment and preserve axons An epidermal membrane periodic scaffold protects axons from damage.

Latest paper is now out and features on the cover of Science Advances 👉 tinyurl.com/vea5862x

Very grateful Igor Bonacossa Pereira, Dat Le & Massimo Hilliard @hilliardlab.bsky.social for their creativity & passion.

Read more about it 👉
tinyurl.com/56awjda5
& 👉 tinyurl.com/2vp8p8uk

Biology and therapeutic potential of extracellular vesicle targeting and uptake - Nature Reviews Molecular Cell Biology Extracellular vesicles are released from almost all cell types and mediate intercellular communication by delivering proteins, nucleic acids and lipids. This Review examines extracellular vesicle upta...

What a great start for 2026 for the EVICAT team!!!
Everything you need to know about extracellular vesicles' recipient cells and how to harness these pathways for therapeutic purposes
Thanks to all co-authors!
bit.ly/3N9InyW

A tribute to Stuart Kornfeld, pioneering #glycotime scientist whose work on mannose-6-phosphate receptor lysosomal trafficking (among many other contributions) was highly influential to our work 🫡

www.jci.org/articles/vie...

New paper out!
We show how mechanosensitive adherens junction proteins link actomyosin contractility to actin assembly using in vitro reconstitution.
Huge congrats to Aurélie Favarin, Rayan Said, & all authors!
In Science Advances: www.science.org/doi/10.1126/...
#actin #myosin #mechanobiology

Optogenetic SH3 nanoclustering of SRC and HCK uncovers the functional specificity of these redundant kinases in macrophages. https://www.biorxiv.org/content/10.64898/2025.12.27.692121v1

Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing - Scientific Reports Scientific Reports - Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing

A comprehensive article for our unique Live-to-HPF CLEM workflow in Scientific Reports!🔬❄️ Thanks to all who supported our long journey!
Images are taken @ImagoSeine @ijmonod.bsky.social @imagerie-gif.bsky.social @i2bcparissaclay.bsky.social @jeoleurope.bsky.social
www.nature.com/articles/s41...

Keratin intermediate filaments mechanically position melanin pigments for genome photoprotection - Nature Cell Biology Benito-Martínez, Salavessa and colleagues show that keratin intermediate filaments and microtubules control the three-dimensional perinuclear position of melanin-containing organelles, shielding the D...

@natcellbio.nature.com - Finally out! Skin cells mechanically protect their genome from UV by positioning melanin above nucleus. Keratin filaments & microtubules drive the process. Led by Silvia Benito-Martinez & @laurasalavessa.bsky.social @membramics-lab.bsky.social www.nature.com/articles/s41...

#LivingArchitectures
We put cells and cytoskeleton filaments on the architecture of the musée d'Orsay.
www.musee-orsay.fr/fr/agenda/ev...
Scientists of the #CytoMorphoLab adapted their protocols to illustrate the questions that keep them awake at night.
-> Two shows on the 24th and 25th of January.

Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...

The result of a fantastic Iwasa-Drubin lab collaboration.

@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...

Assaying the myosin super-relaxed state across muscle types, cells and proteins for understanding muscle biology and use in drug discovery - Nature Protocols The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodolo...

Great collaborative work led by Christopher Toepfer and his team at the University of Oxford

is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social

www.biorxiv.org/content/10.1...

Alternative Splicing of SORBS1 Affects Neuromuscular Junction Integrity in Myotonic Dystrophy Type 1 Background Myotonic dystrophy type 1 (DM1) is a multisystemic neuromuscular disorder characterized by CTG repeat expansion in the 3′ untranslated region of the dystrophia myotonica protein kinase co...

I am thrilled to share our new article entitled 'Alternative splicing of SORBS1 affects neuromuscular junction integrity in myotonic dystrophy type 1 onlinelibrary.wiley.com/doi/10.1002/...

@cecilemartinat.bsky.social

PhD student management tip from his advisor René Couteaux, discoverer of the neuromuscular junction: "*points at 50 cm of lab bench* here's where you'll work. I'll be back in three months to see what you've done"

I'm at the French Society for Myology meeting with @stefvass.bsky.social! He just made a book about the late Michel Fardeau, first president of the society, pioneer of electron microscopy for muscle and muscle disease
en.wikipedia.org/wiki/Michel_...

The Microscopists | Christophe Leterrier (CNRS & Aix-Marseille Université) This time on The Microscopists, we're joined by Christophe Leterrier, team leader at NeuroCyto in Marseille and director of a Nikon Center of Excellence for super-resolution microscopy.Christophe r...

Want to hear a mid-career PI pontificate about science, career and cooking? Today's your lucky day thanks to The Microscopists podcast! Thanks Peter @yorkbioimaging.bsky.social for having me 🎙️🎧
themicroscopists.bitesizebio.com/episodes/chr...

New NeuroCyto preprint about to drop! @wiesner-t.bsky.social 😎⏳

www.sciencedirect.com/science/arti...

check out the recent dispatch on endolysosomes and melano-biology interplay in @currentbiology.bsky.social by @delevoyecedric.bsky.social & @laurasalavessa.bsky.social from @membramics-lab.bsky.social

Adaptor protein complex 2 (AP2) participates in biogenesis and homeostasis of myelin sheaths in the central nervous system Myelination of CNS axons requires oligodendrocytes to undergo extensive morphological changes by producing large amounts of myelin membrane with defined protein composition and structure. The formatio...

If you're interested in trafficking of #myelin constituents in #oligodendrocytes, and of course you are, you may want to check out our new preprint. It's very nice PhD work by @sophiesiems.bsky.social, and we're grateful to all who contributed!

www.biorxiv.org/content/10.1...

Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!

how did I miss this fantastic paper...congrats Marko @kaksonen.bsky.social and team!!

Diving into "le AIS"🤿