Julian Gargiulo

“A #SPADarray in any laser scanning #microscope”. That is the latest effort from a group of people that shared this mantra. Proud of all of them.
@erc.europa.eu
#imagescanningmicroscopy #photonresolvedmicroscopy
#superresolution
#BrightEyes_ERC

doi.org/10.1038/s415...

Happy Pi day to those who like to arrange units by order of magnitude.

Entrevista Miguel Laborde, tecnólogo argentino Miguel Laborde cuenta la situación actual del sistema de ciencia y técnica y su participación en el desarrollo de una pila que usa un submarino español.

Un submarino español usa una pila de hidrógeno basada en un proceso que desarrolló Miguel Laborde.
Acá lo cuenta con @aleberco en @pasaroncosas899

El problema no es el sistema de ciencia y tecnología sino el modelo de desarrollo.

radiocut.fm/audiocut/ent...

Lasers on at UNSAM.

Thrilled to share that my first #micropublication is out! 🧬🔬 I created a user-friendly ImageJ/FIJI plugin for nuclear segmentation and multi-channel fluorescence analysis that doesn't require programming skills.

www.micropublication.org/journals/bio...

‘Scienticide’ in Argentina sparks huge protest by researchers Hundreds of activists wearing gas masks took to the streets to call out their government for slashing science funding.

Milei government is destroying the Argentine scientific system.
We resist

www.nature.com/articles/d41...

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

Getting ready for #TOPFOT 2025
Check the program here
sites.google.com/view/eeoftop...

Read preprints. Cite preprints. Email people and tell them you loved their preprint. Email people you hated their preprint. Embrace preprints, preprints are good.

Our manuscript on accelerating the acquisition rate in oblique plane microscopy (OPM) is online now:

opg.optica.org/boe/fulltext...

Our work addresses the issue that an OPM PSF is tilted, and as such it leaves a lot of "empty space" in Fourier space (FFT of experimental data on the right)... 1/n

Another nice example from the 1st Snouty light-sheet microscope!

-> Here we show a mammalian cell with 2 fluorescent reporters imaged at ~1 volume per second. The speed and gentleness of the microscope make it ideal for 3D tracking and segmentation!

Prepared and imaged by @jlazzaridean.bsky.social

Hi #SnoutClub and microscopy people.
Can you suggest python software for 3D registration?
I want to find the axial shift between two z-stacks
Thanks!!

How could the translation of nanotechnology-based therapies revolutionize cutaneous leishmaniasis treatments? Published in Nanomedicine (Ahead of Print, 2025)

HILO: Hace instantes se publicó nuestro nuevo trabajo del LANSAB-UNAHUR por Magalí di Meglio, Daniela Maza Vega y yo. Es un editorial sobre las chances reales y desafíos de la nanotecnología para tratar la leishmaniasis: www.tandfonline.com/doi/full/10....
1/4

📣SEMINARIO INS
El investigador Alberto Scarpettini charlará sobre nanoestructuras híbridas núcleo-satélite formadas por satélites plasmónicos de oro soportados sobre núcleos magnéticos de óxidos de hierro para la remediación asistida por luz solar de agua que contiene arsénico

Posesiones Ocultonas · Episode

Con @marieldemente.bsky.social y @agmendez.bsky.social grabamos un nuevo episodio de Ocultonas, sobre posesiones, exorcismos y discernimiento de espíritus. No se lo pierdan que quedó geniallll

open.spotify.com/episode/5dte...

YouTube video by xkcd's What If? What if the wise men kept walking after Jesus’s birth?

What if the wise men kept walking after Jesus’s birth?

Watch the latest What If? video collaboration with MinuteEarth!

youtu.be/YL2VNtus4xk

Congratulations Gustavo! Proud to be your friend!

🧪💡🔉📢 We are happy to announce that Phonons 2025 will be held in Buenos Aires, Argentina, from 1 to 5 December 2025 at the University of Buenos Aires (UBA). Checkout the list of the prestigious plenary and invited speakers:

phonons2025.com

On wednesdays we wear gfp 💐🪰

It's been a long journey, only possible thanks to my lovely co-authors who are not (yet) in Bluesky.
Big thanks also to @amsikking.bsky.social , @andrewgyork.bsky.social and
@lumasullo.bsky.social, they have always been there to help.
Stay tuned for new imaging on living Drosophila!
6/6

In addition, we can do 2 colours imaging...
Here the membrane is labelled in green (GFP), and dense core vesicles are labeled in magenta (mCherry).
We also show mitochondria imaging in the manuscript.
5/6

And what about the photobleaching?
We can image the fly for 30 minutes without bleaching it, and can get 10 times more images than with confocal microscopy!
Also, we don't rely on phototoxic pulsed infrared lasers like in 2-photon microscopy.
4/6

We imaged the axonal projections of small lateral ventral neurons (known as s-LNvs) in living adult flies.
The NA=1.1 allowed high spatial resolution.
Here, a line intensity profile showing features as small as 360 nm.
3/6

We used a Single Objective Light-Sheet (#Snouty, for the friends).
It is less invasive than a lattice light sheet because it requires a smaller dissection area on the head´s cuticula, as it is only necessary to accommodate the light cone of a single objective. And it is simpler. 2/6

HIGH-RESOLUTION IN VIVO BRAIN IMAGING OF DROSOPHILA 🪰
New Preprint by Tassara et al.!

www.biorxiv.org/content/10.1...
#FluorescenceMonday #SnoutClub 1/6

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

Microscope objectives can be confusing, and detailed specs (or even basic properties) are often hard to find. Here's an introductory resource to help new builders and instrument designers with the basics. It's a work in progress so feedback is appreciated:

amsikking.github.io/microscope_o...

Oh no! Keep us posted on your new adventures.

For 2-3 color videos doesn't work that well. Any solutions are appreciated