Stefan Mereiter

🚨 PhD position open! 🚨
Join our team at the Medical University of Vienna to study glycan regulation in mammary organoids, milk, and breast cancer.
Details in the image – please share! 🧬✨

Stefan Mereiter erhält Förderung des FWF für internationales Forschungsprojekt Lesen Sie mehr über das Projekt!

Wir gratulieren @smereiter.bsky.social! 👏🔝

Der Postdoc-Researcher an der #MedUniWien & am ‪@imbavienna.bsky.social ‬erhält einen Förderungs-Grant des @fwf-at.bsky.social. Mehr dazu ⤵

1/23 Big news for the #ObenaufLab! So excited to finally share our new study providing another puzzle piece, why immunotherapy fails in many tumors, now out in @Nature 🧵👇 www.nature.com/articles/s41...

Feeling honored to work in a lab with two (!) of 2024’s most cited scientists: mentor Josef Penninger 🧠 and lab colleague Max Kellner 📚 (@clarivate). Max (spot him sitting behind me in yesterday’s lab meeting) is a PhD student 🤯

please don't 🙏

Hi Wouter. Could you please add me too 🙋🏽‍♂️

Thank you for organizing this starter pack! Would love to be added.

12/
Great collaboration between BOKU University, IMBA, MedUni Vienna, and Michigan University! Thank you, J. Helm and the amazing Stadlmann Lab for taking me on this ride!! Together, we’re uncovering new insights into glycans. 🟦 🟡 🔺 🟢 🔶 🟨

Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice - Nature Communications N-glycosylation is vital for biological processes but difficult to analyse. Here, the authors introduce a scalable method to profile N-glycans across 20 mouse tissues, revealing tissue-specific glycos...

11/
In our paper, we showcase a scalable, LC-MS/MS data-driven method combining automated ID, non-targeted profiling, and isomer-sensitive glycan analysis. Dive into the details: doi.org/10.1038/s414...

10/
For those ready to dive deeper: We haven’t even started with the chromatography data! PGC allows us to differentiate N-glycans with the same mass but distinct structures, adding more layers of tissue-specific complexity.

9/
But wait—there was more! After filtering non-glycan spectra and expected glycans, we found spectra representing unexpected glycan structures and mapped them across all tissues! 🔍

8/
Next, we extended SNOG with eSNOG, extracting spectra with special fragment ions to compare glycan structural features like α-Gal, antennary Fuc, Neu5Ac, Neu5Gc, and oligo-Man across tissues.

7/
With SNOG-filtered data, we saw clear, tissue-specific glycan clusters emerge, with high technical reproducibility across replicates. This filtering step was a game-changer!

6/
First up: SNOG-filtering. This automated step removes contaminants (up to 75% in some tissues!) so we can focus on genuine N-glycan signals.

5/
Our approach had to be scalable, non-targeted (no pre-built mass lists, please!), and data-driven. Automated spectral ID would be the icing on the cake! 🧁 We combined PGC analysis with multiple profiling strategies for semi/quantitative N-glycomics.

4/
Faced with an overwhelming amount of LC-MS/MS data, we knew manual annotation was out of the question. Instead, we developed an “unconventional” approach to analyze our N-glycome data in a faster, smarter way. Here’s what we did:

3/
So, we analyzed N-glycans from 20 mouse tissues, generating a comprehensive, structure-resolved N-glycome Atlas. Using PGC columns for isomer separation and an Orbitrap for high-res MS/MS, we assembled a mountain of data. Now what?

2/
Have you ever wondered how many unique N-glycan structures a single organism might create? Or if the sugars on your kidney cells differ from those in your brain?
We did too, and we set out to investigate!

1/
Hi 🦋! Did you know? Glycans—complex sugar structures—decorate almost every protein processed by the ER/Golgi. These N-glycans coat our cells, playing critical roles in cell interactions and immune processes. 🍬 Curious about how they vary? Let’s dive in! #GlycoTime

Great list & thanks for organizing! Would love to join.

Thank you for organizing! Would love to be added.

Could you also add me? Thanks a lot!