Abs Khan

As a side note - I didn't think much of a 100km row over the whole month, but 10km in and I must say I am questioning my judgement/fitness/ability...

HSCB lab rows 100km each for bone cancer research Help Abdullah Khan raise money to support Bone Cancer Research Trust

Different flavour of post from me today!

A bunch of us in the lab at the @imm.ox.ac.uk are taking up Bone Cancer Research Trust (BCRT)'s June rowing challenge to both raise funds and awareness:

www.justgiving.com/fundraising/...

Please share/donate if you can!

Looking forward to it Zoltan - bring me some home cooking !

I loved this course! If Aiden or Natalie are instructing say hi from me!

Thank you 😀

😊🥹

🔥 New Fraticelli lab publication🔥

“Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemia driver mutations”

Now at Cell Stem Cell, with two new figures, in vivo, and sequential mutagenesis data.

Performed with the support of Cris Cancer and @erc.europa.eu

Very kind of you Dianne, appreciated!

Finally finally a big thank you to everyone who has engaged with and showed an interest in implementing the system. I am hopeful that we can make big strides forward as a community as these types of systems advance. Thank you to patients + funders!

. If you're interested in implementing please join: groups.google.com/g/morerganoids
Where we help troubleshoot + share protocols etc.

And this is the playlist:
open.spotify.com/playlist/380...
To which the paper was written for everyone out there who writes/works to very specific music.

A lot of the major therapeutic challenges and many interesting questions are around clonal evolution, chemoresistance etc. To address those well we need longer lived, 'stable' cultures - we see a decline in complexity over time in our static cultures. Watch this space!

I'll end on quoting @kellystevens.bsky.social 'It's not about how far we've come, but about how far we have left to go.' I hope you will agree this is a big step forward, but there are still challenges that I am excited to tackle (funding pending of course).

... you can build in a lot of complexity and work out strategies to retain throughput and scalability.

We present what we believe is a highly complex in vitro model that showcases the potential of human organoid systems. By adapting granular microgels pioneered by colleagues in the bioengineering field (e.g. J Burdick and others), I think we also show that...

Finally that helps us identify an inflammatory pathway (MIF) which we think underpins myeloma mediated inflammation. We show that inhibiting this in myeloma-comBO chimeroids ameliorates markers of inflammation.

We then apply this system as a pre-clinical model to study multiple myeloma - showing that we support primary myeloma cells (notoriously difficult to culture ex vivo) and capture hallmarks of their extensive interactions with bone marrow niches.

In this manuscript we address these to present comBO: a combined bone and lympho-myeloid bone marrow organoid system (I know the acronym is a bit of a reach, but it's stuck now).

(1) Capturing simultaneous lympho- and myelopoiesis in a single culture to better model haematopoiesis. (2) Generating the specialist osteoblastic niche and (3) circumventing the limitations imposed by bulk hydrogel embedding (cost, labour, scalability, automation).

On to the science! Despite recent advances in developing bone marrow organoid systems (our own work in Cancer Discovery (2023) and Nature Protocols (2024) + Frenz-Weissner paper (Nature Methods, 2024), the major challenges (as we see them) remained:

And of course @bethpsaila.bsky.social for all the support (and patience...). A big thank you to the whole HSCB lab for all the support which most often manifests as pranks/coffee/donuts...

... it can still be fun so a huge thank you to everyone who made it so. In particular shout out to @yuqi-shen.bsky.social qi-shen.bsky.social without whose support it would have been impossible to do this. @Sarah Gooding for gentle guidance and excellent collaboration in the myeloma world.

First and foremost, a huge thank you to all co-authors. This has been a tricky project to pull together having moved twice + juggling demands of grant writing/trying to set up a research group. With the support of lovely people in an excellent environment @imm.ox.ac.uk ...

comBO: A combined human bone and lympho-myeloid bone marrow organoid for pre-clinical modelling of haematopoietic disorders The bone marrow supports lifelong blood and immune cell production. Current human bone marrow organoid models do not include both lymphoid and myeloid elements and lack the complexity of stromal cell ...

Good morning everyone, I'm pleased to share our latest advance in bone marrow in vitro modelling:

www.biorxiv.org/content/10.1...

Still 😍🔥🔥🔥🔥

THANK YOU This has been haunting me circa. 1997…
Also I like it, but it’s not a track that does well on endless repeat

Thank you for the reminder to revisit this album and listen obsessively for a few days

Kick off the new year with cutting-edge science. Join the NIC, Prof. Christoph Klein and @abattacks.bsky.social for their webinar on "Organoids" happening on Wednesday, 8 January 2025! www.iseh.org/ISEHWebinars

Definitely attend if you can! Nice to see all the hard work from everyone so nicely presented !!