Arabidopsis lines with modified ascorbate concentrations reveal a link between ascorbate and auxin biosynthesis Ascorbate is the most abundant water-soluble antioxidant in plants, and it is an essential molecule for normal plant development. It is present in all green plants, with very different concentrations ...

Mario's vitamin C study uncovers an interesting link between this essential metabolite and the regulation of local auxin biosynthesis www.biorxiv.org/content/10.1...

Development of this new genetic tool was sparked by a spontaneous discussion at a scientific conference. One two-year NSF EAGER grant to jump-start the work and 5 more years of "side projects" by 18 talented scientists made EBSn possible. Good science takes time! www.biorxiv.org/content/10.1...

Beyond a few bases: methods for large DNA insertion and gene targeting in plants Large, precise DNA insertions remain challenging to achieve in plants due to both biological and technical bottlenecks. This review highlights recent advances in CRISPR-Cas systems, recombinases, and...

Katie Vollen's PhD prelim exam writeup transformed into a nice review article on large DNA manipulation:
onlinelibrary.wiley.com/doi/full/10....
Way to go, Katie!

𝐅𝐨𝐜𝐮𝐬𝐞𝐝 𝐫𝐞𝐯𝐢𝐞𝐰📚
Beyond a few bases: methods for large DNA insertion and gene targeting in plants🧬🌱
📖 doi.org/10.1111/tpj....
🖊️Katie Vollen @alonsostepanovalab.bsky.social
🗺️ @ncstate.bsky.social
Large DNA insertions in plants are challenging, but new tools improve efficiency

If you're struggling to assemble large DNA constructs, check out Chengsong's DASH system, which combines Golden Gate cloning, recombineering, and site-specific recombinases to build multigene constructs of ~100kb.
www.biorxiv.org/content/10.1...