Shih-Hsun Walter Hung

Engineering flowers as a means for public outreach and art!

Dr. @nickdesnoyer.bsky.social reimagines Arabidopsis with new colors and shapes, blending plant biology, synthetic biology and creative arts!

Hear him speak at SB 8.0 at the iGEM Grand Jamboree! jamboree.igem.org/2025/program...

DO-DIFFERENT. @uniofeastanglia

Matt Dickinson #PPATH2025

Coconut lethal-yellowing type phytoplasma — Matt Dickinson #PPATH2025

Next up at #PPATH2025 is Erh-Min @erhminlai all the way from @IPMBSinica Taipei 🇹🇼 / optimising Agrobacterium for plant gene editing; cool use of a RUBY red readout assay

See the preprint doi.org/10.1101/2025...

A Shakespearean title for Jan van Kan our next speaker #PPATH2025 @WageningenUR 🇳🇱 #Botrytis

Now we have Chih-hang Wu @IPMBSinica Taiwan 🇹🇼 at #PPATH2025 — Fast and Furious: What happens after helper NLR immune receptors are activated to cause hypersensitive cell death.

See paper doi.org/10.1101/2025...

My PhD student and my PhD supervisor showed up in matching outfits. Am I supposed to complete the set?

My two favorite Ethiopians 😍

The sequences of 91,366 NLR immune proteins from 230 superasterid genomes are now printed in 931 bound volumes #OpenScience #OpenPlantNLR

Also indexed on
@ZENODO_ORG
as an #OpenPlantNLR #NLRbiology dataset

zenodo.org/records/1472...

Dual-cycle CO2 fixation enhances growth and lipid synthesis in Arabidopsis thaliana Carbon fixation through the Calvin-Benson-Bassham (CBB) cycle accounts for the majority of carbon dioxide (CO2) uptake from the atmosphere. The CBB cycle generates C3 carbohydrates but is inefficient ...

Dual-cycle CO2 fixation enhances growth and lipid synthesis in Arabidopsis thaliana | Science www.science.org/doi/10.1126/...

Atmende Endosymbionten ebnen den Weg in neue Lebensräume: Umweltmetagenome mitochondrien-ähnlicher #Endosymbionten können auch #Sauerstopff atmen und so den Wirten neue Lebenräume eröffnen.
@einzeller.bsky.social & Kolleginnen in #Biospektrum @springernature.com

link.springer.com/epdf/10.1007...

We are excited that the Earth BioGenome Project, a global network of scientists including those from the Sanger Institute, has mapped the second phase of its ambitious plan to sequence all 1.67 million known species on Earth by 2035. 👏

www.sanger.ac.uk/news_item/bi...

About twenty people are gathered around five tables, discussing about biorxiv preprints

Six people with their laptops are sitting around a table and discussing.

Another Preprint Pizza Party has come and passed!

Students, postdocs and RAs from TSL and @johninnescentre.bsky.social gathered to discuss and review recent @biorxivpreprint.bsky.social articles 📝
Pizza kept the spirits high during this challenging task 🍕

#PreprintCommentClub #PreprintPizzaParty

Super-resolution live-cell mapping of protein-protein interactions using chemogenetic split reporters and STED microscopy The ability to map protein-protein interactions (PPI) within living cells at high spatial resolution is essential for unravelling their roles in cellular biology. Although several super-resolution mic...

Super-resolution live-cell mapping of protein-protein interactions using chemogenetic split reporters and STED microscopy
Stephanie Board, Arnaud Gautier
bioRxiv 2025.08.05.668682; doi: doi.org/10.1101/2025...

Engineering non-exponential proliferation in Escherichia coli using functionalized protein aggregates Uncurbed exponential proliferation might not always be required for genetically modified microorganisms, and might even cause unpredictable liabilities in their behavior and impact. We therefore const...

Engineering non-exponential proliferation in Escherichia coli using functionalized protein aggregates
Ronald Van Eyken, Sam Jordens, Dries Oome, Kevin Broux, Abram Aertsen
bioRxiv 2025.08.23.671898; doi: doi.org/10.1101/2025...

1/13 🧵 Today, Bindcraft was published in
@nature.com , one of the most famous AIs in biology for designing protein–protein interactions (PPI). In my opinion. Bindcraft represents one of the most important advances in the post–AlphaFold2 era.

This is a breakthrough for protein science🔥AFAIK this is the largest protein DB, with >100B seqs (3B clustered at 50%). New biology will come from LOGAN: new folds, topologies, etc. You can also improve your AlphaFold models by building better MSAs. Future AI models will also use LOGAN for training

Reductive evolution of the DNA replication machinery in endosymbiotic fungi The molecular machinery for replicating and repairing DNA accurately is critically important for life and highly conserved across the Tree of Life. Here we show that two major lineages of fungi, Glome...

I’m super happy to present the first discoveries of the RIKEN-Cambridge Joint Crop Symbiosis Research Team, based in Japan 🇯🇵

doi.org/10.1101/2025...

A thread 👇

Conserved effectors underpin the virulence of liverwort-isolated Pseudomonas in divergent plants Robinson et al. identify pathogenic Pseudomonas viridiflava in wild Marchantia polymorpha liverworts and interrogate the mechanisms enabling virulence in non-flowering and flowering plants. Their resu...

Conserved effectors underpin the virulence of liverwort-isolated Pseudomonas in divergent plants

Very happy to share the published version of our work on natural Marchantia-Pseudomonas interactions.

Out now in Current Biology: www.cell.com/current-biol...

First of the Friday Flower Concepts series coming tomorrow! 🌹✨

Can you guess the first flower?

"Little Flower"

A Cryo-SEM portrait of my micro-rose depicting the flower as a sculpture of cells, fractured and frozen in time. Full image is 10k x 10k pixels.

To promote the collection, I’ve launched a designer clothing line where synthetic biology meets aesthetics.

I will give out 100 free limited edition shirts to the first who retweet and order with their handle in the name! Available here → nickdesnoyer.com 🧵(6/7)

How can we better understand the plant immune system? In my new @cp-cellhostmicrobe.bsky.social piece, I propose “immune cell states” as a framework for uniting molecular and cellular perspectives.

www.cell.com/cell-host-mi...

Model: Activation and suppression of defences in plant–aphid interactions. Upper left panel: aphid stylet penetration of the cell wall releases oligogalacturonides (OGs), which induce PAMP/DAMP-triggered immunity (PTI/DTI) in a process dependent on BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), and CPK5/6/11 (data herein). The stabilisation of pattern recognition receptors (PRRs) and coreceptors at the plasma membrane may rely on the deubiquitination (DUB) activity of ASSOCIATED MOLECULE WITH THE SH3 DOMAIN OF STAM (AMSHs; Gravino et al., 2024). Upper right panel: the Mp10 effector, introduced by aphids into the cell cytoplasm (Mugford et al., 2016), suppresses OG-induced reactive oxygen species (ROS) (data herein) and flg22-induced PTI (Bos et al., 2010). Mp10 targeting of plant AMSHs is implicated in these processes (Gravino et al., 2024). EDS1 is essential for Mp10-mediated ROS suppression and PRR destabilisation (data herein), acting through an unidentified mechanism (denoted by the double-sided arrow with an asterisk). Lower left panel: effector-triggered immunity (ETI) is activated through EDS1, either directly or indirectly, upon recognition of Mp10 and/or its activities by a TNL (Gravino et al., 2024; Rao et al., 2024). Salicylic acid glucosyltransferase 1 (SGT1) is also required for TNL/ETI activation (Bos et al., 2010). Lower right panel: aphids secrete additional effectors, such as cathepsin B proteins (e.g. CathB6), which target EDS1 to suppress ETI (Liu et al., 2025). Solid arrows, increased activation; solid blunt-ended arrows, increased suppression; Dashed arrows, reduced activation; Dashed -blunt-ended arrows, reduced suppression.

🎉Thrilled to share our latest work, now published in @newphyt.bsky.social!

🧑‍🔬 @matteogravino.bsky.social, @samtmugford.bsky.social, @saskiahogenhout.bsky.social et al., @johninnescentre.bsky.social

#️⃣ #PlantScience #PlantImmunity

📄 Read the full paper👇
nph.onlinelibrary.wiley.com/share/8QSRJ9...

Aphid effectors suppress plant immunity via recruiting defense proteins to processing bodies Aphids hijack plant immunity via p-bodies, but plants fight back with a heat shock protein in an unexpected twist on defense.

Our paper is now online in Science Advances! Congrats @saskiahogenhout.bsky.social @johninnescentre.bsky.social and all coauthors!
www.science.org/doi/10.1126/...

Wow… can’t believe it’s almost a year as a predoc 🧑‍🔬 @johninnescentre.bsky.social
time flies so fast 🤯

Wow 🤩 🙏 @waltershhung

Taiwanese bottarga—烏魚子 (ô͘-hî-á in Taiwanese Hokkien or wū yú zǐ in Mandarin) 🇹🇼

Genome-Wide (GW): an interactive browser for ultrafast chromosome-scale genomics data visualization.

www.nature.com/articles/s41...

New Resource: "Transcription factor binding divergence drives transcriptional and phenotypic variation in maize" rdcu.be/esSwW

Genome-wide comparative analysis of DNA binding for 200 transcription factors in two maize inbred lines.