Maithy (he/him)

By deep characterisation of 186 N-glycan standards, we @thencfg.bsky.social have made the most comprehensive targeted LC-MS glycomic assay to date.
Non-reduced native glycan analysis is key.
We hope this enables anyone with QqQ and interest in #glycotime to measure specific N-glycan structures.

🚀 Over 1k public datasets in @pride-ebi.bsky.social now have direct links to GEO, unlocking matched transcriptomics data! Final stats are dropping this week, but this is a big step toward a unified way to annotate #multiomics and build cross-database references in #PRIDE. 🧬🔗 #FAIRdata

@deutsche-bahn.bsky.social you are the worst!!

Malaria vaccination sees child deaths drop in Kenya’s lake region In Kenya’s lake region, malaria vaccination has contributed to a drop in both mortality and disease severity among kids, experts confirm.

Gute Neuigkeiten zum #Weltmalariatag 2025: Der neue #Impfstoff gegen #Malaria wirkt - so gibt es deutlich weniger schwere Verläufe & Todesfälle bei geimpften Kindern. Es ist ein Schritt voran im Kampf gegen eine der tödlichsten #Infektionskrankheiten. via @gavi.org www.gavi.org/vaccineswork...

Ibaqpy: A scalable Python package for baseline quantification in proteomics leveraging SDRF metadata Intensity-based absolute quantification (iBAQ) is essential in proteomics as it allows for the assessment of a protein's absolute abundance in various…

ibaqpy: A scalable #Python package for baseline quantification in proteomics leveraging #SDRF metadata www.sciencedirect.com/science/arti.... We continue our effort to provide tools and algorithms that leverage SDRF metadata to perform quantitative proteomics analysis.

#Skyline #proteomics #MassSpec
@maccoss.bsky.social Hope everyone that uses Skyline shows their support skyline.ms/project/home...

SpheriCal ESI: 295 - 1,550 Da | Polymer Factory Polymer Factory’s first ESI-designated calibration kit is based on hydrophilic dendritic macromolecules that cover the mass range between 295 and 1,550 Da. We are now offering this product as a pre-launch at small portion size of 5 µg at a beneficial price. Since we are eager to learn about the performance of our product on various platforms and under varying conditions, we offer further discounts for future orders of the fully released product in exchange for your feedback and shared data (spectra, spraying conditions, etc.). What makes SpheriCal® ESI unique?In contrast to other calibration solutions, we offer our calibrants as solid, neat compound. The material is fully soluble in hydrophilic solvents commonly used in ESI. This includes water, alcohols, aqueous mixtures of alcohols, aqueous ACN.Our compounds are “non-sticky” in the ion source and will not tail into your data. At the same time, the compatibility with a wide range of spraying solutions and conditions enables internal calibration.Further, SpheriCal® does not contain any fluorinated components.With a shelf life of years, we expect the 5 µg portions to enable about 100 calibrations.SpheriCal® offers reliable and accurate calibration, without the shelf-life and storage issues associated with protein or peptide-based systems. Simply freeze your aliquots once prepared. In positive mode, our compounds do not need addition of acetic or formic acid and ionize well with traces of Na to yield [M+Na]+. Addition of acid will also yield [M+H]+ in positive mode.In negative mode, addition of acid is required and yields [M+acid-H]- anions. [M-H]- might also be observed.This gives full control to the end user to choose concentration, solvent, addition of acid etc. Componets: C6H12O4; 148.074 g/mol* C12H22O8; 294.131 g/mol C20H36O12; 468.221 g/mol C25H44O16; 600.623 g/mol C30H52O20; 732.305 g/mol C37H62O22; 858.373 g/mol C40H70O25; 950.421 g/mol C50H84O30; 1,164.505 g/mol C65H108O40; 1,528.642 g/mol *Compound 1 varies in intensity depending on hardware and tuning and is not guaranteed to be detectable. Related Publications Advantages of Monodisperse and Chemically Robust “SpheriCal” Polyester Dendrimers as a “Universal” MS Calibrant - J. Am. Soc. Mass Spectrom., 2014, 25, 303-309

Maybe these people can help you, I would suggest asking them if they have any good low mass calibrants www.polymerfactory.com/product-page...

An ‘Impossible’ Disease Outbreak in the Alps In one tiny town, more than a dozen people were diagnosed with the rare neurodegenerative disease ALS. Why?

Fascinating article about a cluster of ALS cases. If you think about it, this is a situation in which the researchers skimmed a large number of variables and identified a correlate which is then considered the potential cause.>

H/t @scientificdiscovery.dev

www.theatlantic.com/health/archi...

I am re-sharing since I don't get the feeling anyone noticed this pearl of wisdom (not by me). People I know who frequently "win" at pitches/proposals are following the Heilmeier formula: from simple communicating up to presenting concisely on project webpages to full on proposals.

Proteomic Analysis of Human Plasma during Intermittent Fasting Intermittent fasting (IF) increases lifespan and decreases metabolic disease phenotypes and cancer risk in model organisms, but the health benefits of IF in humans are less clear. Human plasma derived...

Here's a 3d printed version, pmc.ncbi.nlm.nih.gov/articles/PMC..., you can also order such a holder from GL biosciences.

If you have only 100 ng starting material you should just use stage tips.

How small?

@DGMS Annual MS conference #TeamMassSpec #Göttingen

Reminder that Europepmc.org exists. In case anyone should need it for any reason.

#TeamMassspec Astral peeps, would love to know your experience with the data size, processing softwares and the time it takes. Thanks for the help!

A five-step laboratory workflow diagram for protein analysis and sex determination from tooth enamel. Step 1 shows sample preparation with acid etching and peptide extraction using test tubes. Step 2 depicts peptide desalting using a multi-well plate. Step 3 illustrates a centrifugal vacuum concentrator. Step 4 shows a nano-LC mass spectrometer with nano spray. Step 5 displays data processing on a laptop and spectral analysis for AmelY unique peptides, with a graph and male/female symbols. Each step is connected by arrows showing the sequential workflow."

🦷 Enamel proteins identify a female Byzantine ascetic - challenging assumptions that extreme religious self-punishment was just for men.

In harsh, arid environments where DNA doesn't survive, proteins in tooth enamel can still reveal biological sex.

www.sciencedirect.com/science/arti...

Identifying receptor kinase substrates using an 8,000 peptide kinase client library enriched for conserved phosphorylation sites In eukaryotic organisms, protein kinases regulate diverse protein activities and signaling pathways through phosphorylation of specific protein substrates. Isolating and characterizing kinase substrat...

Identifying receptor kinase substrates using an 8,000 peptide kinase client library enriched for conserved phosphorylation sites #MCP #MassSpec www.mcponline.org/article/S153...

Titrating biotinylated probe and streptavidin conc to find the sweat spot is the key step in high throughput chemoproteomics workflow.. Especially balancing the target(s) specificity and yield.. Liked the heatmap depicting intensity of specific and nonspecific proteins from the pulldown..

PathwayPilot: A User-Friendly Tool for Visualizing and Navigating Metabolic Pathways Metaproteomics, the study of collective proteomes in environmental communities, plays a crucial role in understanding microbial functionalities affecting ecosystems and human health. Pathway analysis ...

PathwayPilot: A User-Friendly Tool for Visualizing and Navigating Metabolic Pathways #MCP www.mcponline.org/article/S153...

Baumarkt in Germany have springs that fit the syringe. I would just suggest to checkout any hardware store. Just take the syringe with you and see if it fits.

Development, optimization and validation of a highly sensitive and selective method for the determination of PFAS in animal-based food Perfluorocarboxylic acids and perfluorosulfonic acids accumulate in food webs, thus posing a serious threat to food safety. The European Food Safety A…

Development, optimization and validation of a highly sensitive and selective method for the determination of PFAS in animal-based food #Chemosphere #MassSpec www.sciencedirect.com/science/arti...

A universal spectrum annotator for complex peptidoforms in mass spectrometry-based proteomics www.biorxiv.org/cont...

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#proteomics #prot-preprint

Even the suppl. tables do not have enough/proper information. :(

I am trying to curate available CO-IP MS data for a protein and am appalled and saddened by the number of publications without MS raw/search data. I did not think this would be the case! #TeamMassSpec

I took biochem in 2001, and for nearly 20 years read amino acid sequences daily… and I never knew Dayhoff named them or even the logic behind things like Q until last Friday (h/t Mike Janech). Also, this is another big Dayhoff moment for me. She was incredible!

#proteomics #bioinformatics

#TeamMassSpec how do you organize your MS raw file storage? Would love to hear your suggestions!

How do you store your Mass Spec raw files?

1️⃣ According to the instrument and month/year
2️⃣ Based on project/date/sample
3️⃣ Based on the user and let them organize
4️⃣ Other

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