Jake Choby

Antibiotics accumulate in Klebsiella pneumoniae liver abscesses but fail to eliminate antibiotic-tolerant populations | PNAS Liver abscesses caused by hypervirulent Klebsiella pneumoniae (hvKp) can lead to severe metastatic complications, with mortality rates ranging from...

Thrilled to share our latest paper on antibiotic treatment failure of Klebsiella pneumoniae liver abscesses

www.pnas.org/doi/full/10....

@drwalkermsbanta.bsky.social

A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics - Nature Microbiology The authors developed a screen to find compounds that modulate intracellular Staphylococcus aureus metabolism and discovered KL1, which sensitizes persisters to antibiotics by reversing host-induced tolerance.

Delighted to share our latest paper - A host-directed adjuvant sensitizes intracellular bacterial persisters to antibiotics. Excellently led by Dr. Kuan-Yi Lu. We think it's a great proof-of-concept that altering immune cell behavior can make antibiotics work better www.nature.com/articles/s41...

Super excited to share our paper online 🚨today🚨 in Cell Host & Microbe‬! Xiaomei Ren @xiaomeiren.bsky.social and Mason Clark @rmasonclark.bsky.social‬ co-led discovery of ecological factors for Acinetobacter baumannii carriage in the gut, a reservoir for pathogen spread. 🎉

tinyurl.com/443kfefk

Thanks to my colleagues, friends, and especially mentors @dweisslab.bsky.social and Eric Skaar for guidance along the way

It's a good time to mention that I’ve just started as an assistant professor at the University of North Carolina SOM, Micro & Immuno, where we will work to understand mechanisms that generate heteroresistance and phenotypic heterogeneity in Enterobacter and other multi-drug resistant Gram- pathogens

Copy number flexibility facilitates heteroresistance to increasing antibiotic pressure and threatens the beta-lactam pipeline Nature Communications - Choby et al. show that dynamic increases in the copy number of preexisting β-lactamase genes in heteroresistance enables resistance of continua of cellular...

Excited to share the work with @dweisslab.bsky.social‬ and great collaborators on how gene amplification generates dynamic heteroresistance to new beta-lactams, like cefiderocol. We hope drug developers will consider heteroresistance during antibiotic development rdcu.be/euijW

So much joy to see everyone today coming together to celebrate 20 years of the Skaar Lab. Thank you to Eric for the leadership and mentorship over the years! 🥰

Congrats! Such a cool story

Zinc-dependent β-lactam resistance at a cost - Nature Microbiology Chemical genomics reveals the fitness cost of bacterial metallo-β-lactamase in zinc-deplete environments, with implications for treatment options.

News & Views 📄 - Zinc-dependent β-lactam resistance at a cost

By Jacob Choby & David Weiss

www.nature.com/articles/s41...

pleased to share our (with @dweisslab.bsky.social) News & Views perspective on this exciting paper
nam11.safelinks.protection.outlook.com?url=https%3A...

Enteropathogenic E. coli (EPEC) Transfers Its Receptor for Intimate Adherence into Mammalian Cells Enteropathogenic E. coli (EPEC) belongs to a group of bacterial pathogens that induce epithelial cell actin rearrangements resulting in pedestal formation beneath adherent bacteria. This requires the secretion of specific virulence proteins needed for signal transduction and intimate adherence. EPEC interaction induces tyrosine phosphorylation of a protein in the host membrane, Hp90, which is the receptor for the EPEC outer membrane protein, intimin. Hp90–intimin interaction is essential for intimate attachment and pedestal formation.

Discussed this awesome paper as a student years ago and still think about it www.cell.com/fulltext/S00...