Phage papers

Resolution of acute rejection in a bilateral double-lung transplanted cystic fibrosis patient following phage intervention - PubMed This case underscores the potential of phage therapy to complement traditional treatments in managing chronic bacterial infections in CF patients, though further studies are needed to optimize treatment protocols and understand the role of phages in modulating the bacterial population.

Resolution of acute rejection in a bilateral double-lung transplanted cystic fibrosis patient following phage intervention.

DOI: 10.1128/asmcr.00058-24

https://pubmed.ncbi.nlm.nih.gov/41244966/

Isolation, Identification and Typing of Nine Strains of Brucella in Arun Banner - PubMed People involved in raising and trafficking sheep constitute a high-risk group for brucellosis. The strains isolated were B. melitensis, with ST8 as the predominant type identified by MLST typing. Attention should also be given to the discovery of new alleles.

Isolation, Identification and Typing of Nine Strains of Brucella in Arun Banner.

DOI: 10.1002/vms3.70694

https://pubmed.ncbi.nlm.nih.gov/41251272/

Polyvalent phage GSP004 recognizes O-antigen polysaccharide receptors in Salmonella and Escherichia coli through tail fiber protein ORF208 - PubMed The majority of phages are capable of lysing only specific bacterial strains within a single species, and polyvalent phages with cross-genus lytic capability are relatively rare. In this study, we isolated a polyvalent phage, GSP004, from swine farm wastewater, which exhibited cross-genus lytic acti …

Polyvalent phage GSP004 recognizes O-antigen polysaccharide receptors in Salmonella and Escherichia coli through tail fiber protein ORF208.

DOI: 10.1128/jvi.00810-25

https://pubmed.ncbi.nlm.nih.gov/41251352/

Hypervirulent Klebsiella pneumoniae causing aortitis retains its capsule and mucoviscosity and remains genotypically and phenotypically stable over time - PubMed Aortitis due to hypervirulent Klebsiella pneumoniae (hvKp) in a Belarusian male from central New York is described. Isolates at the time of diagnosis (Kp031824-1, Kp031824-2) and after 12 weeks of antimicrobial therapy (Kp070124) were characterized. Kp070124 was genomically and phenotypically unchan …

Hypervirulent Klebsiella pneumoniae causing aortitis retains its capsule and mucoviscosity and remains genotypically and phenotypically stable over time.

DOI: 10.1038/s41598-025-23563-1

https://pubmed.ncbi.nlm.nih.gov/41233465/

Bacteriophage density influences the rate of resistance evolution - PubMed The antagonistic relationship between bacteria and bacteriophages (phages) drives genetic changes that result in phage resistance. Phage resistance mutations arise in natural microbial communities and during the treatment of diseases with phages (phage therapy), making it important to understand the …

Bacteriophage density influences the rate of resistance evolution.

DOI: 10.1101/2025.10.05.680490

https://pubmed.ncbi.nlm.nih.gov/41256655/

Structural basis for Lamassu-based antiviral immunity and its evolution from DNA repair machinery - PubMed Bacterial immune systems exhibit remarkable diversity and modularity, as a consequence of the continuous selective pressures imposed by phage predation. Despite recent mechanistic advances, the evolutionary origins of many antiphage immune systems remain elusive, especially for those that encode hom …

Structural basis for Lamassu-based antiviral immunity and its evolution from DNA repair machinery.

DOI: 10.1073/pnas.2519643122

https://pubmed.ncbi.nlm.nih.gov/41252147/

A novel long-acting C5a-blocking cyclic peptide prevents sepsis-induced organ dysfunction via effective blockade of the inflammatory cascade - PubMed Sepsis is a life-threatening syndrome characterized by dysregulated host responses to infection, leading to severe organ dysfunction and a high mortality rate. Reducing the incidence of sepsis is of paramount importance. Given that sepsis-associated drugs largely fail in clinical trials, in this pro …

A novel long-acting C5a-blocking cyclic peptide prevents sepsis-induced organ dysfunction via effective blockade of the inflammatory cascade.

DOI: 10.1038/s41392-025-02457-8

https://pubmed.ncbi.nlm.nih.gov/41188260/

Novel lytic phages improve the antibiofilm activity of dalbavancin, daptomycin, and fosfomycin against vancomycin-resistant enterococci - PubMed Periprosthetic joint infections (PJI) caused by <i>Enterococcus</i> spp., especially vancomycin-resistant strains (VRE), are challenging to treat due to biofilm tolerance and limited antibiotic options. Bacteriophages offer a promising adjunct through targeted and biofilm-disrupting activity. This s …

Novel lytic phages improve the antibiofilm activity of dalbavancin, daptomycin, and fosfomycin against vancomycin-resistant enterococci.

DOI: 10.1128/spectrum.01818-25

https://pubmed.ncbi.nlm.nih.gov/41258817/

A previously undescribed archaeal virus suppresses host immunity - PubMed Extremophilic archaea can have chronic viral infections that are well-tolerated by the hosts and may potentially protect against more lethal infections. Here we show that a natural Haloferax strain (48N), is chronically infected by a lemon-shaped virus. This viral infection is not cleared spontaneou …

A previously undescribed archaeal virus suppresses host immunity.

DOI: 10.1038/s44319-025-00540-3

https://pubmed.ncbi.nlm.nih.gov/41249581/

Nanobody-based recombinant antivenom for cobra, mamba and rinkhals bites - PubMed Each year, snakebite envenoming claims thousands of lives and causes severe injury to victims across sub-Saharan Africa, many of whom depend on antivenoms derived from animal plasma as their sole treatment option¹. Traditional antivenoms are expensive, can cause adverse immunological reac …

Nanobody-based recombinant antivenom for cobra, mamba and rinkhals bites.

DOI: 10.1038/s41586-025-09661-0

https://pubmed.ncbi.nlm.nih.gov/41162699/

Structural basis for Lamassu-based antiviral immunity and its evolution from DNA repair machinery - PubMed Bacterial immune systems exhibit remarkable diversity and modularity, as a consequence of the continuous selective pressures imposed by phage predation. Despite recent mechanistic advances, the evolutionary origins of many antiphage immune systems remain elusive, especially for those that encode hom …

Structural basis for Lamassu-based antiviral immunity and its evolution from DNA repair machinery.

DOI: 10.1073/pnas.2519643122

https://pubmed.ncbi.nlm.nih.gov/41252147/

PhIP-Seq uncovers marked heterogeneity in Acute Rheumatic Fever Autoantibodies - PubMed Acute rheumatic fever (ARF) and associated rheumatic heart disease are serious sequelae of a Group A Streptococcus (GAS/Strep A) infection. Autoantibodies are thought to contribute to pathogenesis, with deeper exploration of the autoantibody repertoire needed to improve mechanistic understanding and …

PhIP-Seq uncovers marked heterogeneity in Acute Rheumatic Fever Autoantibodies.

DOI: 10.1172/jci.insight.196619

https://pubmed.ncbi.nlm.nih.gov/41252216/

gcMeta 2025: a global repository of metagenome-assembled genomes enabling cross-ecosystem microbial discovery and function research - PubMed The rapid growth of metagenomic sequencing has generated an unprecedented wealth of metagenome-assembled genomes (MAGs), transforming opportunities for microbial discovery and functional characterization. Yet, full utilization of these resources has been constrained by heterogeneous data generation …

gcMeta 2025: a global repository of metagenome-assembled genomes enabling cross-ecosystem microbial discovery and function research.

DOI: 10.1093/nar/gkaf1115

https://pubmed.ncbi.nlm.nih.gov/41171134/

Advances in combating antimicrobial resistance in MRSA: a comprehensive review on nanotechnology and phage therapy - PubMed Integrating advanced nanotechnology and phage therapy into healthcare systems could transform the MRSA treatment landscape. Future research guided by systems biology and personalized medicine principles will be crucial for mitigating antimicrobial resistance and ensuring equitable access to novel th …

Advances in combating antimicrobial resistance in MRSA: a comprehensive review on nanotechnology and phage therapy.

DOI: 10.1007/s10482-025-02208-3

https://pubmed.ncbi.nlm.nih.gov/41247565/

Optimizing phage-antibiotic combinations: impact of administration order against daptomycin non-susceptible (DNS) MRSA clinical isolates - PubMed The rise of bacterial resistance has driven the exploration of novel therapies, such as bacteriophage-antibiotic cocktails (PACs), which have shown <i>in vitro</i> promise against resistant pathogens, including daptomycin non-susceptible-methicillin-resistant <i>Staphylococcus aureus</i> (DNS-MRSA) …

Optimizing phage-antibiotic combinations: impact of administration order against daptomycin non-susceptible (DNS) MRSA clinical isolates.

DOI: 10.1128/aac.00699-25

https://pubmed.ncbi.nlm.nih.gov/41251374/

Successful treatment of macrolide-resistant Mycobacterium abscessus infection using multi-drug regimens including dual β-lactams and phage therapy: case reports in two children - PubMed Successful treatment of macrolide-resistant <i>M. abscessus</i> infections in children can be achieved using multi-drug regimens including novel dual β-lactam combinations and phage therapy. Dose adjustment with therapeutic drug monitoring can ameliorate some drug-induced toxicities in children.

Successful treatment of macrolide-resistant Mycobacterium abscessus infection using multi-drug regimens including dual β-lactams and phage therapy: case reports in two children.

DOI: 10.1128/asmcr.00087-24

https://pubmed.ncbi.nlm.nih.gov/41244981/

Unexplored diversity and molecular genetic signatures of chimallin and phuz encoding phages - PubMed Nucleus-forming phages are a class of jumbo phages with the capacity to form a protein nuclear shell during infection within a bacterial host. This protein nuclear shell protects the replication of the phage genome by excluding host defense factors. However, the lack of genetic characterization of t …

Unexplored diversity and molecular genetic signatures of chimallin and phuz encoding phages.

DOI: 10.1186/s12864-025-12246-w

https://pubmed.ncbi.nlm.nih.gov/41249907/

Polyvalent phage GSP004 recognizes O-antigen polysaccharide receptors in Salmonella and Escherichia coli through tail fiber protein ORF208 - PubMed The majority of phages are capable of lysing only specific bacterial strains within a single species, and polyvalent phages with cross-genus lytic capability are relatively rare. In this study, we isolated a polyvalent phage, GSP004, from swine farm wastewater, which exhibited cross-genus lytic acti …

Polyvalent phage GSP004 recognizes O-antigen polysaccharide receptors in Salmonella and Escherichia coli through tail fiber protein ORF208.

DOI: 10.1128/jvi.00810-25

https://pubmed.ncbi.nlm.nih.gov/41251352/

Unexplored diversity and molecular genetic signatures of chimallin and phuz encoding phages - PubMed Nucleus-forming phages are a class of jumbo phages with the capacity to form a protein nuclear shell during infection within a bacterial host. This protein nuclear shell protects the replication of the phage genome by excluding host defense factors. However, the lack of genetic characterization of t …

Unexplored diversity and molecular genetic signatures of chimallin and phuz encoding phages.

DOI: 10.1186/s12864-025-12246-w

https://pubmed.ncbi.nlm.nih.gov/41249907/

An evolutionary approach to predict the orientation of CRISPR arrays - PubMed CRISPR-Cas is a defense system of bacteria and archaea against phages. Parts of the foreign DNA, called spacers, are incorporated into the CRISPR array which constitutes the immune memory. The orientation of CRISPR arrays is crucial for analyzing and understanding the functionality of CRISPR systems …

An evolutionary approach to predict the orientation of CRISPR arrays.

DOI: 10.1371/journal.pcbi.1013706

https://pubmed.ncbi.nlm.nih.gov/41252463/

Isolation and genomic analysis of seven phages active against carbapenem-resistant Klebsiella pneumoniae - PubMed The genomic sequences of seven phages isolated from municipal sewage in Rochester, Minnesota, USA, using <i>Klebsiella pneumoniae</i> strains (<i>bla</i>_KPC carriers) as hosts are reported. These phages are lytic and belong to the <i>Ackermannviridae, Drexlerviridae</i>, and <i>Zobellviri</i> …

Isolation and genomic analysis of seven phages active against carbapenem-resistant Klebsiella pneumoniae.

DOI: 10.1128/mra.00768-25

https://pubmed.ncbi.nlm.nih.gov/41251356/

Genomic insights into novel ST7947 carbapenem-resistant hypervirulent Klebsiella pneumoniae: a threat from an Indian hospital setting - PubMed BB-7 represents a novel CR-hvKp strain with a unique ST7947 genotype, exhibiting co-resistance and hypervirulence, raising concern for public health. The association of high-risk resistance plasmids with virulence factors underscores the urgent need for enhanced genomic surveillance, infection contr …

Genomic insights into novel ST7947 carbapenem-resistant hypervirulent Klebsiella pneumoniae: a threat from an Indian hospital setting.

DOI: 10.1186/s12941-025-00834-3

https://pubmed.ncbi.nlm.nih.gov/41250062/

Isolation and genomic analysis of seven phages active against carbapenem-resistant Klebsiella pneumoniae - PubMed The genomic sequences of seven phages isolated from municipal sewage in Rochester, Minnesota, USA, using <i>Klebsiella pneumoniae</i> strains (<i>bla</i>_KPC carriers) as hosts are reported. These phages are lytic and belong to the <i>Ackermannviridae, Drexlerviridae</i>, and <i>Zobellviri</i> …

Isolation and genomic analysis of seven phages active against carbapenem-resistant Klebsiella pneumoniae.

DOI: 10.1128/mra.00768-25

https://pubmed.ncbi.nlm.nih.gov/41251356/

Detection and genetic diversity of Wolbachia and its associated prophage WO in mosquito populations from Ethiopia - PubMed Arboviral diseases transmitted by mosquitoes, including dengue, Chikungunya, Zika, yellow fever, West Nile virus (WNV), and Rift Valley fever (RVF), pose a significant public health challenge globally, particularly impacting populations in low and middle-income countries. Conventional mosquito contr …

Detection and genetic diversity of Wolbachia and its associated prophage WO in mosquito populations from Ethiopia.

DOI: 10.1038/s41598-025-23896-x

https://pubmed.ncbi.nlm.nih.gov/41249293/

ABCD-type phage cocktail targeting distinct LPS receptor sites demonstrates superior efficacy against multidrug-resistant Salmonella - PubMed The narrow host range of phages poses a limitation in addressing multidrug-resistant bacteria, whereas phage cocktail therapy, targeting multiple bacterial receptors, broadens the phage host spectrum. This study establishes a comprehensive Salmonella phages repository through nationwide surveillance …

ABCD-type phage cocktail targeting distinct LPS receptor sites demonstrates superior efficacy against multidrug-resistant Salmonella.

DOI: 10.1016/j.jhazmat.2025.140435

https://pubmed.ncbi.nlm.nih.gov/41240830/

Bacteriophage observations and evolution - PubMed Bacteriophages are classified into one order and 13 families. Over 5100 phages have been examined in the electron microscope since 1959. At least 4950 phages (96%) are tailed. They constitute the order Caudovirales and three families. Siphoviridae or phages with long, noncontractile tails predominat …

Bacteriophage observations and evolution.

https://pubmed.ncbi.nlm.nih.gov/12798228/

Phage-conjugated chlorin e6: A strategy overcoming phage resistance in biofilm eradication and wound infection therapy of carbapenem-resistant Acinetobacter baumannii - PubMed Carbapenem-Resistant Acinetobacter baumannii (CRAB) frequently causes biofilm-related infections that can exhibit extreme resistance to antibiotic therapy. Phage therapy shows promise as an alternative treatment, yet bacteria may develop resistance to it with prolonged use. Phage-photosensitizer com …

Phage-conjugated chlorin e6: A strategy overcoming phage resistance in biofilm eradication and wound infection therapy of carbapenem-resistant Acinetobacter baumannii.

DOI: 10.1016/j.jphotobiol.2025.113302

https://pubmed.ncbi.nlm.nih.gov/41213229/

CAR T cells targeting B7H3 demonstrate potent preclinical activity against AML and ESCC - PubMed T cells engineered to express chimeric antigen receptors (CARs) are a promising modality to treat refractory cancers. CD19 CAR-T therapy has achieved remarkable responses in against B-cell lymphomas, however, challenges persist for acute myeloid leukemia (AML) and solid malignancies. B7H3 is an immu …

CAR T cells targeting B7H3 demonstrate potent preclinical activity against AML and ESCC.

DOI: 10.1016/j.intimp.2025.115806

https://pubmed.ncbi.nlm.nih.gov/41213188/

Regulation of phage therapy medicinal products: developments, challenges, and opportunities - PubMed Due to their biological properties, bacteriophages represent a regulatory specialty and, at the same time, a challenge with regard to medicinal product approval. Established European guidelines on pharmaceutical quality, preclinical development, and clinical development are only partially applicable …

Regulation of phage therapy medicinal products: developments, challenges, and opportunities.

DOI: 10.3389/fcimb.2025.1631359

https://pubmed.ncbi.nlm.nih.gov/41234515/

Isolation and genome annotation of Rowlf, a novel cluster EG bacteriophage with a purple acid phosphatase-like gene - PubMed Isolated from grass thatch in Pocatello, Idaho, <i>Microbacterium foliorum</i> phage Rowlf has a siphovirus morphology and is presumed to be a lytic bacteriophage. Rowlf has a 62,044 bp genome with 104 putative genes, including a purple acid phosphatase-like gene found in leguminous plants and bacte …

Isolation and genome annotation of Rowlf, a novel cluster EG bacteriophage with a purple acid phosphatase-like gene.

DOI: 10.1128/mra.00314-25

https://pubmed.ncbi.nlm.nih.gov/40693759/