New preprint out!
www.biorxiv.org/content/10.6...
One of the most salient lessons I learned in all my adventures with screening and protein engineering is the importance of context. The context in which you test a gene or protein determines what function it has.
Principles of in situ protein sequencing: expansion microscopy-adapted Edman degradation and amino acid recognition https://www.biorxiv.org/content/10.64898/2026.01.29.702630v1
This is just the beginning and I’m excited to keep pushing this technology forward in the years ahead.
I’m deeply grateful to my wonderful co-authors, collaborators, and my PhD mentor Dr. Ed Boyden for their support throughout this journey.
Here we establish principles for in situ protein sequencing by demonstrating that peptide sequencing and amino acid recognition are possible within expansion microscopy gels. This is a foundational step toward the long-term goal of single-molecule protein mapping in intact biological specimens.
What if we could locate, sequence, and identify every protein in a cell? This would unlock a transformative way to study biology and disease.
That question shaped my PhD, and I’m excited to share our new preprint on the Principles of In Situ Protein Sequencing, now live on bioRxiv.
🎉 #SMPS4 Group Photo! 🎉
A huge thank you to all the brilliant minds who joined us and made this event unforgettable. Your passion and insights are what drive this community forward. Here's to pushing the boundaries of science together! 🧬✨
We learned so much from Camille Mitchell's session about:Towards in situ protein sequencing with Expansion Microscopy using in-gel
Edman degradation and N-terminal amino acid binders #SMPS4
