S Saif Hasan

Does 30mg/ml refer to 30 mg of total protein per ml, or is it the wet pellet mass per ml? I guess doesn't matter once the optimization is done. Thanks.

Yes! Mass photometry is exactly the plan. Thanks for the suggestion.

We are thinking of trying LMNG-CHS. Would love to hear advice on the Expi293 cell mass to detergent ratio for solubilization. Or protein to detergent ratio. Thank you.

Hello Colleagues. We are purifying a complex of two #membrane proteins from Expi293 cells. We used 1% DDM for solubilization, and 0.02% during chromatography. The complex shows both subunits on the gel but runs really small on SEC. It should have 3 copies of one subunit and 2 of the other.

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Here is a starter pack for CryoEM/ET enthousiasts
To be completed
go.bsky.app/JmQksC

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A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs - Nature Communications Dey et al. use structure-function methods to show that partial mimicry of the coatomer-binding motif in the SARS-CoV-2 spike is crucial for its release post coatomer-dependent delivery, thus ensuring ...

Hello, I am an Assistant Professor at the Univ of Maryland. We investigate #coronavirus glycoprotein trafficking, using #cryoem #cryoet #xraycrystallography and functional tools. Here is one of our no-longer-recent publications. Great to see a vibrant community!

www.nature.com/articles/s41...