CellComM Lab

I can't imagine a better day of the week to publish this than #FluorescenceFriday: PathoPlex is finally published! 🎉

This presents a revolutionary jump from 4-6 to 100+ markers per sample! 🤯

Read the full story here:
www.nature.com/articles/s41...

#HotFromThePress !!

What if instead of analyzing 4-6 pathology markers you could check >100 in the *same* sample?

@maltekuehl.com & co @puellesv.bsky.social lab introduce #PathoPlex, method that could even predict disease

Full www.nature.com/articles/s41...
@focalplane.bsky.social @nature.com 🧪🔬

Excited to be starting my research group as a tenure track Presidential Young Professor at the Mechanobiology Institute @mbisg.bsky.social, and the Dept of Physiology at @nusingapore.bsky.social in the fall! More about our work on vascular mechanobiology in health & disease mechano-medicine-lab.com

This is why we love #cellmigration

#Leukocytes: #Neutrophils (cyan) #Macrophages (orange) chemotaxis.

I couldn't share it before the retirement of @zebrafishrock.bsky.social, but cool #microscopy in #zebrafish continue to rock !

#scicomm #scisky @cellcommlab.bsky.social @focalplane.bsky.social 🧪🔬

🧵 Neural networks meet cell migration in Valencia!

Hi Bluesky! 👋 I'm Javier, computational biologist @cellcommlab.bsky.social l working at the intersection of AI and cell biology 🖥️🧫

A very late #FluorescenceFriday.

Reposting one of my favorite timelapses from the past year: EB3-mScarlet3 in a HeLa cell, look at those microtubules grow!

Immune cell profiling reveals diverse niches of immune residents of the enteric nervous system and potential neuroimmune interactions
www.pnas.org/doi/10.1073/...

Image Source: https://perso.ens-lyon.fr/thomas.budzinski/pictures.html and https://plus.maths.org/content/random-surfaces-universe#maths "This picture represents a uniform triangulation of the sphere with over 30,000 vertices. Image by Thomas Budzinski, used by permission."

Read and enjoy this short article about interesting mathematical shapes (plus.maths.org/content/rand...) to learn about random surfaces and the secrets of the Universe!

♾️ #iTeachMath #Science‪ #Maths
Do not miss @plusmaths.bsky.social
Image by Thomas Budzinski (perso.ens-lyon.fr/thomas.budzi...)

Microscopy preprints - applications in biology - FocalPlane Microscopy preprints - applications in biology - News

📢Our latest preprint list is now up on FocalPlane.
This week’s list focusses on research using microscopy tools to answer questions in biology. As always, please let us know if there are other preprints that you’d like to see included.
focalplane.biologists.com/2025/06/27/m...

The 2nd AI4Life Challenge is live!
Calling the AI & bioimaging community to tackle a key microscopy challenge: removing noise while preserving detail.

📦 Paired noisy/clean datasets
📈 Ground-truth evaluation
🧠 DL focus

Build, test, compete 👉 ai4life.eurobioimaging.eu/challenge-2/

Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

#FluorescenceFriday

#Mitochondria, powerhouse of the #cell were labeled (cyan) and it's possible to visualize how they reach *every* corner to provide energy.

All recorded under the microscope @cellcommlab.bsky.social

#scicomm #sciencesky #microscopy #organelles @focalplane.bsky.social 🧪🔬

This is why we love #cell dynamics !

Look at this #macrophage branching exploring the surroundings videoed under the microscope

This what immune cells do in our body in search of bacteria, viruses and death cells

#microscopy #cellbio #sciencesky @focalplane.bsky.social @cellcommlab.bsky.social 🧪🔬

Very excited to share the first preprint from the lab:
www.biorxiv.org/cgi/content/...
We investigated neural crest migration all the way from head to tail of the Zebrafish embryo and we found their nuclei suffer dramatic deformations.
Want to know of they cope with feeling squeezed? 🐟🐠🐟

#CellMigration
Join us today @dgz.bsky.social !!
See you in 1 h (zoom id and password in the pic below)

Yes ! Thanks for the poke @fadelvalle.bsky.social
You can check some of Julie Theriot's paper on membrane protrusions ;)
I'll try to find the ref to put them in the thread for you, @anhhle2702.bsky.social

Join us for this @dgz.bsky.social seminar online with very cool talks about the Cell biology of the immune system

There will be of course some #CellMigration of leukocytes ;)

Speakers: @liz-kurtz.bsky.social, #ZahraaA, @labliston.bsky.social and #StefanU

The next #DGZ Focus Workshop on #CellBiology of the #ImmuneSystem will take place on April 29, organized by @pjsaez.bsky.social & Evi Kiermaier – join us! 🧑‍🔬🧑‍🔬🧬

An oldie (10 y!) but goodie: cellular interaction at the top! #cellcomm

Video of a T cell (orange) interacting with a target cell (blue)

From Chen et al. @ Betzig lab (@hhmijanelia.bsky.social) game changing paper !!

Full science.org/doi/10.1126/... @focalplane.bsky.social #scicomm #microscopy 🧪🔬

Mysterious increase in the motility of these #lysosomes

After adding a non-stimuli treatment the lysosomes (cyan) got crazy (see color coded tracks)
Maybe #mechanosignaling triggered by shear stress?

#Microscopy + #Cells for #FluorescenceFriday 🧪🔬
#SciSky #scicomm #CellBio

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...

And finally a funny trick for actin aficionados: label actin with different probes and use their differential staining properties to identify actin structures within cells: www.biorxiv.org/content/10.1...

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

Great #SciArt 👇🏽

#CellMigration for #FluorescenceFriday 🧪🔬

If a metastatic #cancer cell is offered 2 paths it decides very fast, bc it does matter where it goes...

OTOH, if a non-cancer #cell is offered 2 options it takes some time to explore and decide.

More in the paper: www.nature.com/articles/s41...

How cells decide where to go? #CellMigration

Last year we published our 1st paper @cellcommlab.bsky.social, showing how actin-based membrane oscillations define a new direction ( #cancer & non-cancer #cells )

Full www.nature.com/articles/s41...
@naturephysics.bsky.social @focalplane.bsky.social 🧪🔬

Adherens junctions are protein complex with cadherins, catenins & other, like vinculin.

In composition, epithelial & endothelial AJs differ in the type of cadherin, where epithelial has E-cad & endothelial has VE-cad.

But are they different in structure? 🤔

elifesciences.org/articles/84364

#CellMigration meets #SciArt

Naomi Wee (naomiwee.com) did this great movie to explain how amoeboid migration works (also mesenchymal & cancer migration)

Wanna know what happens if this leukocyte chooses the wrong path? Watch this !

www.youtube.com/watch?v=mPwn... @focalplane.bsky.social #SciSky

#CellMigration aficionados, it would be cool to have a similar video for amoeboid cell migration too, no?

Well come tomorrow and see the nice work done by Naomi Wee.

Early teaser below, you can already see membrane dynamics and the leading edge vs uropod shape

#GRCCellMigration #SciArt #GRC

Warming up for the upcoming #GRCCellMigration

For me the best video to explain adhesion-dependent #CellMigration by Felix Donghwi Son

More in the website felixvis.artstation.com #sciart #scicomm #ScienceSky #SciSky #cytoskeleton #FocalAdhesion