CellComM Lab

Torque-based immune cell chemotaxis in complex environments Directed migration in chemical gradients is crucial to the immune response, yet how immune cells navigate complex tissues remains incompletely understood. Using in vitro migration assays and theoretic...

Hot from the #preprint

Leukocyte + #CellMigration + physics = cool

Microtubules enable torque-based steering during dendritic cell migration.

Actomyosin contractility is required for noise-modulation strategy used by neutrophils.

Full @biorxivpreprint.bsky.social www.biorxiv.org/content/10.1...

Still posting cytoskeleton videos, it seems. Actin this time.

Sample: Lifeact-eGFP in HeLa cells.
Modality: Airyscan confocal

Timestamp is mm:ss and the scale bar is 5 µm.

It’s time to talk about epithelial geometry and cancer. How can the architecture of an epithelium affect how tumors will grow and spread? In this thread, @jorgealmagro.bsky.social

Synchronized development of zebrafish embryos immobilized by snake venom (alpha-bungarotoxin). Credit to Dr. Ian Swinburne. #ZebrafishZunday

Even if "provisionally" accepted, those are the best words/news I've heard for quite a while...

Here some #blebs to "provisionally" cellebrate.
Let's see this #cell dance under the microscope.

@focalplane.bsky.social #fluorescencefriday #microscopy 🧪🔬

I'm back at regulardly doing some live imaging so I have new little videos of actin in the immune synapse.

The law of the jungle.
Interactions of cells in a collective lead to global rotation.
In 80% of the case HUVEC cells turn clockwise.
How many cells does it take for this to happen?

#AsgardArchaea team, led by @archaeal.bsky.social fr @texasscience.bsky.social — sequencing the DNA collected fr mouth of Rio de la Plata to the continental shelf of Uruguay to detect Asgards, a group of single-celled organisms & our closest microbial relatives on the tree of life. bit.ly/3MdniTH

Do you want to do some frontier of knowledge research? There's still time to register for the next webinar of @hfspo.bsky.social

By far one of the best agencies I've worked with for my beloved HFSP project, even if it's a bumpy road...no risk no glory...

www.hfsp.org/hfsp-events/...

What a crazy cool paper! First author @pierreucla.bsky.social with a large crew knocked it out of the park. (GIF below from @the.3i.social LLS) Quantifying cell traction forces at the single-fiber scale in 3D: An approach based on deformable photopolymerized fiber arrays www.pnas.org/doi/10.1073/...

I won't talk about NMeth @natmethods.nature.com forever (probably), but I do want to brag a bit about the November focus issue on cell segmentation and tracking, which is many ways is my last hurrah (and final editorial) for the journal. I am so proud of these papers!! www.nature.com/nmeth/volume...

This week's #FluorescenceFriday, we'll be treated with one of the classical models of #devbio, the neural crest cells. Here is a beautiful video of neural crest cells with GFP-tagged focal adhesion kinase (🔵) and LifeAct-RFP (🟣) migrating on a Fibronectin matrix.
📹: Adam Shellard

I love to see the endoplasmic reticulum (ER, cyan) hitchhiking over microtubules (MTs, orange).

Also the membrane dynamics are lovely.

It's been a while, but here we are for a late #fluorescencefriday with some #microscopy
#scicomm #scisky @cellcommlab.bsky.social focalplane.bsky.social 🧪🔬

Research engineer in Bioimage Analysis for the researchers of the inIdEx FORMULA Hi all We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? The position is in the Instit...

Job alert!

We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks?

See also on the forum:
forum.image.sc/t/research-e...

🚨 My lab is hiring at all levels!

Interested in animal origins & evolutionary cell biology?

I'm recruiting a postdoc, PhD students & a research assistant to study the molecular evolution of cell adhesion using marine invertebrates + comparative genomics.

🔗: clarkelab.com/join/

Please repost!

Interested in doing exciting research on #epithelial #plasticity in the #kidney or #intestine?

Then please check out our recent job ads for #PhD and #Postdoc positions!

www.nature.com/naturecareer...
www.nature.com/naturecareer...
www.nature.com/naturecareer...
www.nature.com/naturecareer...

I can't imagine a better day of the week to publish this than #FluorescenceFriday: PathoPlex is finally published! 🎉

This presents a revolutionary jump from 4-6 to 100+ markers per sample! 🤯

Read the full story here:
www.nature.com/articles/s41...

#HotFromThePress !!

What if instead of analyzing 4-6 pathology markers you could check >100 in the *same* sample?

@maltekuehl.com & co @puellesv.bsky.social lab introduce #PathoPlex, method that could even predict disease

Full www.nature.com/articles/s41...
@focalplane.bsky.social @nature.com 🧪🔬

Excited to be starting my research group as a tenure track Presidential Young Professor at the Mechanobiology Institute @mbisg.bsky.social, and the Dept of Physiology at @nusingapore.bsky.social in the fall! More about our work on vascular mechanobiology in health & disease mechano-medicine-lab.com

This is why we love #cellmigration

#Leukocytes: #Neutrophils (cyan) #Macrophages (orange) chemotaxis.

I couldn't share it before the retirement of @zebrafishrock.bsky.social, but cool #microscopy in #zebrafish continue to rock !

#scicomm #scisky @cellcommlab.bsky.social @focalplane.bsky.social 🧪🔬

🧵 Neural networks meet cell migration in Valencia!

Hi Bluesky! 👋 I'm Javier, computational biologist @cellcommlab.bsky.social l working at the intersection of AI and cell biology 🖥️🧫

A very late #FluorescenceFriday.

Reposting one of my favorite timelapses from the past year: EB3-mScarlet3 in a HeLa cell, look at those microtubules grow!

Immune cell profiling reveals diverse niches of immune residents of the enteric nervous system and potential neuroimmune interactions
www.pnas.org/doi/10.1073/...

Image Source: https://perso.ens-lyon.fr/thomas.budzinski/pictures.html and https://plus.maths.org/content/random-surfaces-universe#maths "This picture represents a uniform triangulation of the sphere with over 30,000 vertices. Image by Thomas Budzinski, used by permission."

Read and enjoy this short article about interesting mathematical shapes (plus.maths.org/content/rand...) to learn about random surfaces and the secrets of the Universe!

♾️ #iTeachMath #Science‪ #Maths
Do not miss @plusmaths.bsky.social
Image by Thomas Budzinski (perso.ens-lyon.fr/thomas.budzi...)

Microscopy preprints - applications in biology - FocalPlane Microscopy preprints - applications in biology - News

📢Our latest preprint list is now up on FocalPlane.
This week’s list focusses on research using microscopy tools to answer questions in biology. As always, please let us know if there are other preprints that you’d like to see included.
focalplane.biologists.com/2025/06/27/m...

The 2nd AI4Life Challenge is live!
Calling the AI & bioimaging community to tackle a key microscopy challenge: removing noise while preserving detail.

📦 Paired noisy/clean datasets
📈 Ground-truth evaluation
🧠 DL focus

Build, test, compete 👉 ai4life.eurobioimaging.eu/challenge-2/

Fig. 4 Super-resolution microscopy in live and fixed cells with Rhobin. (A) Rhobin transiently binds rhodamine molecules and thereby constantly replenishes signal lost through photobleaching of dyes. (B-D) No-wash STED microscopy of live U2OS cells transiently expressing LifeAct-Rhobin2 labeled with 100 nM JFX650. (C, D) Zoom-ins of regions highlighted in (B) imaged either with confocal or STED microscopy. Scale bar: 20 μm (B) and 10 μm (C, D). (E-H) Rhobin enables timelapse STED microscopy of fast ER dynamics with minimal signal loss. U2OS stably expressing N-terminal tag fusions of SEC61B were stained with 1 μM Halo-JFX650 (HaloTag7, reHaloF) or 2 μM SiRhP (Rhobin2) and imaged at a frame rate of 0.387 fps for at least 100 time points. (F, G) Selected frames from timelapse acquisitions of Rhobin2:SEC61B labeled with SiRhP (F) or HaloTag7:SEC61B labeled with Halo-JFX650 (G). Scale bar: 2 μm. (H) Signal .CC-BY-NC 4.0 International licenseavailable under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprintthis version posted June 25, 2025.;https://doi.org/10.1101/2025.06.24.661379doi:bioRxiv preprint 22 loss during timelapse imaging. Mean ± SD signal inside cells over time for indicated constructs. (I-K) PAINT-type super-resolution microscopy with Rhobin. Rhobin2-SEC61B was transiently expressed in U2OS cells and labeled with 5 nM SiRhP after chemical fixation. Imaging under no- wash conditions and near-TIRF illumination. (I) Repeated, but transient binding of SiRhP molecules to Rhobin2 at low nanomolar concentrations can be observed as intensity bursts in intensity time traces extracted from a diffraction-limited area. See movie S3 for raw data of binding-induced blinking. (J) Sum image across 10,000 frames of an image stack, simulating a diffraction-limited image. (K). Reconstructed image from localized molecules in the full stack.

De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy by Bo Huang and team: www.biorxiv.org/content/10.1...

#FluorescenceFriday

#Mitochondria, powerhouse of the #cell were labeled (cyan) and it's possible to visualize how they reach *every* corner to provide energy.

All recorded under the microscope @cellcommlab.bsky.social

#scicomm #sciencesky #microscopy #organelles @focalplane.bsky.social 🧪🔬

This is why we love #cell dynamics !

Look at this #macrophage branching exploring the surroundings videoed under the microscope

This what immune cells do in our body in search of bacteria, viruses and death cells

#microscopy #cellbio #sciencesky @focalplane.bsky.social @cellcommlab.bsky.social 🧪🔬

Very excited to share the first preprint from the lab:
www.biorxiv.org/cgi/content/...
We investigated neural crest migration all the way from head to tail of the Zebrafish embryo and we found their nuclei suffer dramatic deformations.
Want to know of they cope with feeling squeezed? 🐟🐠🐟