Marcel Dickmanns | 🌿❄️🔬

No didn't look yet. Was thinking to pull one by one via uniprot ID (sequence and MSA), but agree a full db would be nice.

Could pull MSAs from the DB now and save couple hours of CPU time.

You can get MSAs directly from AlphaFold DB now (alphafold.ebi.ac.uk). I also missed the FoldSeek implementation, which lets you search for structurally similar proteins direktly.

This saves some clicking around. Neat!

Mhh, shouln't the meme be the other way round? Especially with new algos and the immense GPU/HPC tech they require.

From Borgese et al. 2006 https://doi.org/10.1016/j.ceb.2006.06.008

Haha yes! Some more contenders in rod outer segments, paracrystalline arrays in mitos and ER, funky OSER forms. But hard to argue with stacks of pancakes sustaining global oxygen and food supply🥞🏆

123 new genomes you say?

Time to update some sequence alignments 🥲

Thylakoids, the prettiest membrane structures in planty cryoET, in full glory:

This is the way!
Next step: incentives for reviewing preprints.

I am excited to share our new preprint on the CAGE complex, a mysterious hollow protein complex that I first saw years ago while surveying Tetrahymena ciliary lysate www.biorxiv.org/content/10.1... #cilia #protistsonsky 🧬🧪

Correlative MS Imaging for cellular identification and analysis of in situ cryo-ET https://www.biorxiv.org/content/10.1101/2025.09.16.676641v1

La-Proteina from NVidia to do co-design of fully atomistic protein structures (sequence + side-chains + backbone) for up to 800 residues is now open source with permissive licensing

🖥️ github.com/NVIDIA-Digit...
🗎 arxiv.org/abs/2507.09466

Trouble imaging actin in ExM? Meet HAK-Actin, a probe for U-ExM, cryo-ExM & iU-ExM. Enables post-expansion labeling for max signal. www.biorxiv.org/content/10.1...
Soon at @spirochrome.com
Led by O.Mercey and @lreymond.bsky.social, in collab with @dudinlab.bsky.social and @marinelap.bsky.social

H2B-mRFPruby, scale bar: 20µm
#live-imaging

Haha, agreed! 😁✌️

Awesome, congrats Kate! And thanks for taking the time to share advice with pesky non-aussie students as well;)

Our latest on brassinosteroid signaling: Ligand binding spectrum, specificity & selectivity of all BR receptors in Arabidopsis. By @albertocaregnato.bsky.social , @hmchen93.bsky.social and our great chemistry collaborators Mirek Kvasnica, Jana Oklestkova and Mirek Strnad.

Unexpectedly, @jurgjn.bsky.social found that running Alphafold3 predictions for protein interactions can yield ipTM scores that are more predictive of true interactions when run in pools of proteins instead of pairwise predictions. Presumably, this reflects some sort of "competition effect".

Thanks Ben, that means a lot!
You heard this before but you guys checking in on us helped immensely to keep it going. To more #teamtomo 🤝 #PlantScience (and other photosynthetic critters) 🥂

Uh-oh.

Yupp, same issue. Embedding font didn't help. In the end, I folded and changed everything to boring arial.

Another plant glutamate receptor (GLR) involved in defense response via calcium signaling. This one senses insect eggs. Pretty cool!

Very nice, congrats! The GUS and iGluSnFR patterns are beautiful.

Thanks a lot! Frequently stared at your ET data when we were trying to make sense of the features - beautiful paper leading the way.

Ah wow, totally missed that. Looks amazing! Did you germinate them on-grid? Thanks a lot for sharing. Looking forward to check out the tomos.

In situ architecture of plasmodesmata suggests mechanisms controlling intercellular exchange Plasmodesmata are nanoscopic channels that traverse plant cell walls, establishing membrane and cytoplasmic continuity between adjacent cells to enable direct intercellular exchange. Although numerous...

Mossy sneak peek is here now: doi.org/10.1101/2025...

Hope we will get to see that "particular tissue" data one day! Too much work to live in the drawers.

Thanks Ana!:)

Thanks for sharing Ivan! Physco is just gorgeous.

Thanks a lot Florent!
Honestly, it felt impossible more than once, but came together eventually. Of course lots to uncover still, both with and without tomogaphy.

Thanks a lot Christine!
It absolutely amazes me how early in-plastic micrographs already captured some of these features (Ding '92, Cook '94, Ehlers in multiple works, etc). Its been great fun to go back with fresh eyes and certainty to compare.

Thank you Jose!