Andy Moore

Continuous self-repair protects vimentin intermediate filaments from fragmentation | PNAS Intermediate filaments are key regulators of cell mechanics. Vimentin, a type of intermediate filament expressed in mesenchymal cells and involved ...

Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...

I double checked the metadata and I’m 90% sure it’s not the sun.

Zeiss 880 w/ airyscan

Not discussed so much but those tubules pressed up against the nuclear envelope are certainly unique.

Thank you! This is an airyscan stack.

Thank you! Yup, good old COS-7.

This is a cumulative maximum intensity projection movie of the endoplasmic reticulum labeled with the membrane marker mEmerald-Sec61B.

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

Is it just me or do these cells look a lot like the americas?

This is a HeLa

Close! This is a HeLa expressing lifeact-egfp. It’s an odd looking hela to be fair

In the context of our @reviewcommons.org revision process, I'm happy to announce Microscopy Nodes v2.2.0!
This packs lots of new fun features, including new color management 🌈, clearer transparency handling 🫥, custom default settings 🔧 and more!
Preprint at doi.org/10.1101/2025...

📣 We’re thrilled to announce the judging panel for the 2025 #NikonSmallWorld photomicrography and Small World in Motion video competitions!

🔗 Click to learn about this year's judges: bit.ly/43hmGBq

#Microscopy #Imaging #SciComm #SciArt

Actin sun.

FilaBuster - Vimentin IF fragmentation

In the classical view of mitotic cell division, the spindle apparatus maintains principal control of chromosome capture and alignment. Any breakdown in spindle function can result in chromosome mis-segregation, producing daughter cells with abnormal chromosome number.

I’m excited to announce that my paper describing non-canonical mitotic mechanisms in the early mouse embryo is out in @science.org ! (link at end of 🧵)

Congratulations!!!

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells.

www.biorxiv.org/content/10.1...

FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly https://www.biorxiv.org/content/10.1101/2025.04.20.649718v1

Shout out to the Janelia CellMap team @cellmap.bsky.social and all our amazing collaborators!

In the paper, we introduce and characterize a new tool for tracking single vimentin IFs in live cells. After noticing single filaments moving independently within ostensibly tight bundles, we used FIB-SEM to take a closer look at vimentin bundle organization and vimentin-microtubule interactions.

What started as a brief chat with @buvansr.bsky.social and @vgelfand.bsky.social at the 2023 CellBio meeting @ascbiology.bsky.social has grown into our published collaborative work on single vimentin IF dynamics and organization in cells. Love when stuff like that happens.

Challenging the view on #vimentin! our latest work shows VIF is an active, dynamic filament loosely held together

rupress.org/jcb/article/...

Thanks to @vgelfand.bsky.social @aaandmoore.bsky.social @nu-bsa.bsky.social @hhmijanelia.bsky.social @jcb.org #NUCDB #nikon #CrestOptics #livecellimaging

Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM Vimentin intermediate filaments display active movement throughout the cell, with individual filaments within a bundle moving independently, as demonstrate

Vimentin filament transport and organization revealed by single-particle tracking and 3D FIB-SEM rupress.org/jcb/article-...

Poor little vimentin stick man 😱

A couple of months later than expected, but I'm finally gearing up to share *FilaBuster* - our approach for light-mediated IF disassembly. Stay tuned!

Endogenously tagged vimentin-mEmerald (orange) and F-tractin-mCherry (actin filaments, gray) in a live COS-7 cell imaged with Airyscan microscopy.

Vimentin (orange) and actin filaments (gray) in a COS-7. Airyscan images of the cytoskeleton always bring me joy. I square root transformed the vimentin so you can see the dimmer filaments out at the edge.

Hey #chemsky and beyond, with all that is going on, I am curious what expensive, high margin molecules we could provide to the community for free. What things do you use all the time, cost way too much, and would make a difference? Alexa Fluor 488 NHS? Other dyes? Other molecule?

I wasn't talking about pubmed. You can still search for the word "diversity" there as far as I can tell. I was referring to the search tool on the nih.gov website.