Andy Moore

I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

What's up Bluesky....here's some live-cell imaging taken in my lab

is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social

www.biorxiv.org/content/10.1...

it’s a little transparent, but that’s the scale bar in the bottom left corner. 5 micrometers.

Still posting cytoskeleton videos, it seems. Actin this time.

Sample: Lifeact-eGFP in HeLa cells.
Modality: Airyscan confocal

Timestamp is mm:ss and the scale bar is 5 µm.

24FPS_VimentinCos7GIF_4320p_ultraHQ This is "24FPS_VimentinCos7GIF_4320p_ultraHQ" by Andy Moore on Vimeo, the home for high quality videos and the people who love them.

vimeo.com/1139537563?s...

I uploaded a version of that vimentin IF movie to my vimeo page. It's still compressed, but looks a lot better. Best results when you set the quality to 4k.

Yup - imaged it yesterday on a laser scanning confocal.

Yup!

Fun fact - this protein belongs to the same family as the hard keratins found in all hair (including the hair you might find in a shower drain).

We should promote IFs and septins to position 1 and 2. Actin filaments and microtubules have had their day in the sun. We can just start calling them the third and fourth components 😂

Sure - these are vimentin intermediate filaments which are part of the cell’s internal scaffolding. They confer mechanical resilience to cells and , among other things, help to anchor and position organelles like mitochondria. These filaments are part of the same family that includes keratins.

Not rude at all - appreciate the suggestion. I’ll give it a shot.

Ah sorry for the lack of context. Cos7 cells are a cultured cell line originally derived from African green monkey kidney cells. They are exceptionally flat at the cell periphery so imagers like to use them. This one is a bit of a monster - it’s about 5x larger than normal.

Love it. I acquired the movie at about 10 seconds per frame and the whole thing is just over 15 minutes.

I mean…does this work? I’m at my wits end.

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology

In memoriam: Malcom A.S. Moore (1944–2025) - Leukemia Leukemia - In memoriam: Malcom A.S. Moore (1944–2025)

www.nature.com/articles/s41...
My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.

Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...

📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti...

#Euro-IF

Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...

Thanks! This was actually all dyes. Mito tracker green, SPY555-Tubulin, and SPY650-FastAct-X.

Whoops forgot to add that - 5 microns

It’s sped up - the timer is minutes:seconds

This is an airyscan confocal movie of mitochondria (white) moving around in a mouse astrocyte. Actin filaments are in orange and microtubules are in blue.

Continuous self-repair protects vimentin intermediate filaments from fragmentation | PNAS Intermediate filaments are key regulators of cell mechanics. Vimentin, a type of intermediate filament expressed in mesenchymal cells and involved ...

Intermediate filaments join microtubules in the self-repair club! Great work with in vitro vimentin filaments from @cecileleduc.bsky.social @romet-jegou-lab.bsky.social et al. in @pnas.org: doi.org/10.1073/pnas...

I double checked the metadata and I’m 90% sure it’s not the sun.

Zeiss 880 w/ airyscan

Not discussed so much but those tubules pressed up against the nuclear envelope are certainly unique.

Thank you! This is an airyscan stack.