Cryo Mariena

I do enjoy when I get to extrapolate work knowledge and apply it to every day life situations. Anything from diagnosing an issue at home, to ensuring a family member tosses their compromised treat because of awful recrystallization.

it was probably OK from a (nasty) microorganism POV. But that the texture, flavor, and quality of the ice cream had been damaged as it would recystallize very slowly and not have its original integrity

Funny enough, thinking in terms of cold and sample prep and ice behavior, the other day my ex husband left his ice cream in the refrigerator overnight. He asked about whether it was still OK to eat. I knew, from experiments done here (by my immediate supervisor), that

It finally paid off to think in terms of ice and temps. I also saved work a service call by finding out an issue and troubleshooting it myself.

I think in terms of ice. But by no means am I Jacques Dubochet or Marc Adrian (RIP). So, it felt pretty good when The Boss came by to ask a question about temperatures and phase changes. It made my day.

Eventually I became the mom of not one, but two, and even 3 microscopes that could do cryo, and the vitrification device. I still manage some of those.

Lena wanted to do ALL the things, but wanted to have an extra set of hands who would assist in guiding her lab members, troubleshooting, baking, repairing, etc

Many moons ago I remember telling Lena that I was only good for cryo (as in, cryo was pretty much all I did). I remember she said that it was exactly what was needed, because she didn't have enough hands or time to do all the work that was needed (care for holders, show people how to use them, etc)

CryoET of microbes inside an animal organ? Yes it’s possible! Check out our new preprint showing how we did it! What an amazing collaboration with amazing scientists who made this possible!

Today is the 10th anniversary of the acceptance of the Themis. In that time @davidamuller.bsky.social has established (and broken) records. Lena created a whole new level of cryo research. @bhgoodge.bsky.social will join is to push the limits. Mama STEM is missed

I was going to make an appointment to get my hair cut this weekend 😄!!! Miss you, my friend!

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated.

mrc.tal.net/vx/appcentre...

And, I probably won't travel home this winter, because not only are the flight schedules atrocious, they cost an arm and a leg, even with family chipping in. I'm anticipating being in a foul mood this winter.

Nearly $60 more per pay period (semi-monthly) to cover my son and I under my job's health insurance plan. Private plan and my employer covers a TON of the plan. I didn't vote for this fuckery.

When, after a couple of weeks of back and forth, imaging, and confusion, the kindest (and most hilarious NP) tells you: not cancer! Phewwwww. The best part is sharing the news with the fam and work peeps who have been most understanding and kind.

Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
1/🧵

gorgeous!!!

We have a preprint of a book chapter for you: Structural Analysis of Membrane Proteins in Cell-Derived Microvesicles.

We describe how to image membrane proteins in mid-sized vesicles using cryo-electron tomography

zenodo.org/records/1717...

Dose-efficient cryo-electron microscopy for thick samples using tilt- corrected scanning transmission electron microscopy - Nature Methods Tilt-corrected bright-field scanning transmission electron microscopy offers enhanced cryogenic electron microscopy contrast and substantial improvement in dose efficiency for thick samples such as bacterial cells and large organelles, while still being able to perform single-particle analysis.

Congratulations to this fantastic group of people: www.nature.com/articles/s41...

#sheliveson #tcbf

Boltz v2.2.1 out. A few improvements including support for .pdb templates, better treatment of stereochemistry in guidance potentials, and improved documentation. As always a great thank you to all those in the community who contributed via PR, raising issues or directly reporting issues to us.

Caltech Mourns the Passing of President Emeritus and Nobel Laureate David Baltimore David Baltimore, President Emeritus and the Judge Shirley Hufstedler Professor of Biology, Emeritus at Caltech and a co-recipient of the 1975 Nobel Prize in Physiology or Medicine, passed away on Sept...

Mourning the loss of David Baltimore, a Nobel laureate and President Emeritus of Caltech. His groundbreaking work on reverse transcriptase reshaped our understanding of genetics and paved the way for advances in cancer and HIV research. A true giant in science. www.caltech.edu/about/news/c...

Hi CryoEM Researchers - Here is a grid box for your 6mm HPF samples - The grid box has 2 pockets, each of which can hold a sandwich of 2 6mm HPF planchets or a single 6mm planchet - Offered in a variety of colors/pack sizes - A choice of lid types too - zurl.co/a5V2n

that is awesome!!! Many congrats 🎉

When I arrived to NYC as a postdoc, NYSBDG was my first local meeting, and I was blown way by the world-class quality of the science.

It's a great opportunity to meet the local #structbio & #cryoem community, and it's coming up in two weeks, so register now at the eventbrite link below!

Sr Scientist I/II Informatics - Peptide Design Company Description: AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to ...

Opportunity for computational chemist for cyclic peptide design at AbbVie (North Chicago USA) #CADD #CompChem #cheminformatics #AI #ML #ChemJobs #chemsky 🧪
jobs.smartrecruiters.com/AbbVie/37439...

Density animation generated using ChimeraX, using "cootmode" display settings from chimerax-trimmings.

Movie recorded thusly:

cofr showpivot false; movie record; rock y 30; wait 600; movie encode ~/Desktop/rock_movie_more.mp4 framerate 40; stop

George M. Sheldrick (1942–2025) In memory of George Sheldrick.

A tribute to George Sheldrick and SHELX and the impact on macromolecular and small-molecule crystallography

journals.iucr.org/a/issues/202...

Graduate Field Coordinator-Department of Chemistry and Chemical Biology Graduate Field Coordinator-Department of Chemistry and Chemical Biology The Opportunity The Department of Chemistry and Chemical Biology is seeking a dedicated and detail-oriented Graduate Field Coord...

Hello BlueSky 🦋 I'm the Director of Graduate Studies in Cornell Chemistry. We're hiring a new Graduate Field Coordinator to help support our graduate program. Please apply or repost if you know someone great! tinyurl.com/3znxx4bh

So the crazy thing here is the company this is supposed to be about is Corning. The company famous for its glass research. And the across the board metric is a measure of how similar individual atom positions are in their CRYSTAL structures. So much is nuts here, but that should have been a red flag

Guuuurl, same. Good to know re: forearms. And I'm so sorry for the scrapes and pain 😭😭