George Chiduza

😂😂

Kangfu Peng, Hong Zhang et al. show that scramblase-attenuated #Celegans ATG-9 mutants rescue the #autophagy defect and lysosome damage caused by nondegradative autolysosomes. This uncovers the role of ATG-9 in regulating #lysosome function & integrity. rupress.org/jcb/article/...

Google Colab

Run BioEmu in Colab - just click "Runtime → Run all"! Our notebook uses ColabFold to generate MSAs, BioEmu to predict trajectories, and Foldseek to cluster conformations.
Thanks @jjimenezluna.bsky.social for the help!
🌐 colab.research.google.com/github/sokry...
📄 www.biorxiv.org/content/10.1...

"This paper represents a unique approach to automatically identifying multiple different proteins in 3D tomographic data. What’s exciting is that it doesn’t just find the proteins we know about – it also helps reveal ones we weren’t aware of." Dr Mark Basham, the Rosalind Franklin Institute

Researchers at the Rosalind Franklin Institute, The Alan Turing Institute, and STFC Scientific Computing have developed a new machine learning model that will improve the detection of proteins in 3D images, such as those obtained by cryo-electron tomography.
Read the full update here: zurl.co/1Z0PZ

This will never stop blowing my mind.

Structural biology is in an era of dynamics & assemblies but turning raw experimental data into atomic models at scale remains challenging. @minhuanli.bsky.social and I present ROCKET🚀: an AlphaFold augmentation that integrates crystallographic and cryoEM/ET data with room for more! 1/14.

Another day another structural biology AI based tool 😎

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Arty image of plasma membrane with ion channel embedded

We are currently advertising our AI #ionchannel modelling PhD Studentship. Fully funded (sadly only for UK based students). Please repost or mention to any potential victims hmmmm, *applicants* you know?
www.findaphd.com/phds/project...

Richardbj.bsky.social

2024 S2C2 Single-Particle Cryo-EM Image Processing Workshop - YouTube This playlist contains six recordings covering single particle cryo-EM data processing in CryoSPARC from the 2024 Single-Particle Cryo-EM Image Processing Wo...

1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM.

www.youtube.com/playlist?lis...

I have received a Wellcome Discovery Award to study the initiation of eukaryotic DNA replication, with 8 years of funding at the Francis Crick Institute.

Looking for a postdoc in molecular mechanisms, cryo-EM, cryo-FIB and in situ structure? Contact me now!

3 postdoc positions advertised soon.

Can you add me to the list please?

Visualizing the modification landscape of the human 60S ribosomal subunit at close to atomic resolution Abstract. Chemical modifications of ribosomal RNAs (rRNAs) and proteins expand their topological repertoire, and together with the plethora of bound ligand

One of those #cryoEM papers were using "near-atomic resolution" in the title would be entirely ok. 😉
academic.oup.com/nar/advance-...

Congratulations 👏🏾👏🏾👏🏾

High-resolution template matching of the human ribosome in situ by Lippincott-Schwartz Lab @hhmijanelia.bsky.social @hhmi.bsky.social. >40 conformations within together reveal molecular dynamics of the elongation cycle with previously unknown bending and “spring-like” motion.

Thank you!

What is the source of the text please?

I am about to be recruiting two post-docs to work on cryoEM and cryoET of amyloid aggregates... Anyone interested please drop me a line while the wheels of our HR department turn.............

I am super excited to share our latest work on the native in-cell organization of the mitochondrial respiratory chain 🥳
Using cryo-electron tomography 🔬, we show how the respiratory complexes (and other complexes) are organized inside native mitochondria! 
www.biorxiv.org/content/10.1...

EMBL Jobs We offer a wide range of challenging scientific and non-scientific positions at all of our European locations and encourage applications from international candidates at all career levels.

My first post on Blue Sky - moving over !

For all post-docs out there - fantastic opportunity @EMBL. Independent group leader position: Core funding, state-of-the-art core facilities, access to fantastic Ph.D. students, and of course amazing colleagues. Please apply www.embl.org/jobs/positio...

An AF3-like model available with an MIT license

“Our model and code are released under MIT License, and can be freely used for both academic and commercial purposes.”

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/
G. Napolitano,A. Esposito, and A. Ballabio.

First bluesky post! About time.

This week, our manuscript revealing the structure, inhibition, and mechanism of human Ceramide Synthase 6 was finally published in NSMB.

We combined cryo-EM and mass spectrometry to reveal its ping-pong mechanism. Check it out!
doi.org/10.1038/s415...

Hey, can you add me as well please

Hi, can you please add me too!

Haven't seen one, good idea though. I've seen a #cryoEM.

As my lab's first post on Bluesky. If you are a UK Structural Biologist please come to our one day meeting on Friday 6th December
Lots of talk slots to be selected from abstracts.
Deadline for abstracts is TOMORROW: Friday 15th Nov.
bit.ly/4fDEqfZ

#cryoEM #science

Just recently moved from X/twitter