Jonathan Weissman Lab

Congratulations to James Numez and collaborators for their RENDER platform for delivering epigenetic editors using engineered VLPs. RENDER broadly advances our ability to with critical fundamental and therapeutic applications.
biorxiv.org/content/10.1...

One key component of Perturb-Multi is in vivo Perturb-seq of formaldehyde-fixed tissue, enabled by the Flex reagents from
@10xGenomics.

Perturb-Multi generates rich, multimodal data that can generate fundamentally new insights about the impact of genetic perturbations on tissue function. Check it out!

We used lentiviral delivery of sgRNAs to create genetic mosaic organs, separately knocking out hundreds of genes in the liver of a single mouse.

Perturb-Multi is a platform for pooled genetic screens in intact mammalian tissue with spatial (MERFISH and multiplexed immunofluorescence) and scRNA-seq-based phenotyping.

A platform for multimodal in vivo pooled genetic screens reveals regulators of liver function Organ function requires coordinated activities of thousands of genes in distinct, spatially organized cell types. Understanding the basis of emergent tissue function requires approaches to dissect the...

In collaboration with
@weallen1
and Xiaowei Zhuang, we have developed Perturb-multi: biorxiv.org/content/10.1...