Boris Louis 🧪🔬

Smartphone-based super resolution🔬

Microscopes are generally costly equipment, limiting availability in remote areas

Can we make a high-quality microscope cheaply?

This paper makes that vision more real
tinyurl.com/defxwzjf

With a cost below 350 euros, it delivers single-molecule sensitivity

Microscopy building day 7-9
First images and final checks

We finally have a working microscope 🔬

After installing the camera, we tackled hardware software communications using @micro-manager.bsky.social

Then we performed usual checks:
1) fluorescent marker
2) scale bar
3) bead solution (PSF)

Advancing Super-Resolution Microscopy: Recent Innovations in Commercial Instruments Abstract. Super-resolution microscopy techniques have accelerated scientific progress, enabling researchers to explore cellular structures and dynamics wit

We have a new review out! In this Microscopy & Microanalysis piece, Louisa Mezache and I survey some of the latest commercially available microscope technologies: Nikon NSPARC, Re-scan Gaia, CSR Biotech MI-SIM, refinements to STED, SMLM from Bruker and Abbelight… Check it out with free access:

A universal AI-powered pipeline enables precise 3D analysis of cell morphology and topology within organoid structures.

www.nature.com/articles/s41...

I never imagined I would see something like this happen to federally funded research in the US. I am heartbroken for all the researchers whose grants have been terminated and devastated for all the patients whose current (and future) treatments are being impacted 🧪 💔

How we analyze porous network @rochalab.bsky.social 🔬🧪

1) Adaptive thresholding
2)Distance map calculation
3)Watershed transformation
4)Quantification
5)3D rendering

Code : github.com/BorisLouis/S...

Paper: pubs.rsc.org/en/content/a...

#Microscopy #Optics #SciComm #ImageProcessing

An interesting article from Microscopy and Analysis (by Wiley Analytical science)🧪🔬

Useful checklist if you are looking to build a new optical system or currently improving one !

analyticalscience.wiley.com/content/arti...

Good observation! Yes in this state it would be an issue, we just finished the alignment, we will run a few test and check the imaging before finalizing the setup which include making protection around the system 🙏

I think one is from Saruman and the other from Sauron?
Not sure what is the use of Saruman's one though 😅

Deaths from heart disease down 75%, that’s NIH.

Deaths from stroke down 75%, that’s NIH.

HIV/AIDS no longer a death sentence, that’s NIH.

99% of FDA approved drugs in the last decade, that’s NIH.

Please show this video to anyone who doesn’t understand why the NIH is so important.

1) two vertical support rods, simple Thorlabs posts that reminded us the Two Towers from Lord of the Rings.

2) A fully caged system giving a high rigidity to the setup and reinforcing the stability of the parts that are hanging in the air.

But going upright comes with challenges, not only because it was our first time building such microscope:

📏 The objective lens has to be placed significantly higher to leave room for a sample stage below and potential illumination sources.

💥 That makes stability a real issue.

⚙️ Our solution:

Most research microscopes are inverted

𝐒𝐨 𝐰𝐡𝐲 𝐮𝐩𝐫𝐢𝐠𝐡𝐭?

Because we’re working with photonic chips in which we have to couple an optical fiber in, quite accurately which is not possible with the sample upside down.

... and that means flipping the whole optical setup upside-down (literally).

🧙‍♂️𝐋𝐨𝐫𝐝 𝐨𝐟 𝐭𝐡𝐞 𝐑𝐢𝐧𝐠𝐬: 𝐓𝐡𝐞 𝐓𝐰𝐨 𝐓𝐨𝐰𝐞𝐫𝐬 – 𝐌𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐞 𝐄𝐝𝐢𝐭𝐢𝐨𝐧 🔬 🧪

Meet our latest creation @hofkenslab.bsky.social and @rochalab.bsky.social labs:

A fully custom upright microscope built entirely from scratch, with no microscope body and no commercial frame.

#Optics #SciComm #Microscopy

Their prototype uses a retractable plasma blade fuelled by compressed liquid propane and oxygen, reaching temperatures around 4,000°F, hot enough to cut through steel. 🤯 While it's not yet practical for duelling Sith Lords, it's a remarkable engineering feat.

𝐌𝐚𝐲 𝐭𝐡𝐞 𝟒𝐭𝐡 𝐁𝐞 𝐖𝐢𝐭𝐡 𝐘𝐨𝐮 🔦⚔️

On this Star Wars Day, I revisited Hacksmith Entertainment Ltd. impressive attempt at creating a real-life lightsaber. If you are into starwars and science, you will probably have seen it !

youtube.com/clip/UgkxTLb...

This looks awesome! A new open source optical design software! 😱

Has anyone used it? Is it good? 😁

optiland.readthedocs.io/en/latest/in...

Having both high resolution structural information 🔬and functional information 🧪 (in this case, tension) is very challenging, very cool work!
#Microscopy #optics

Unveiling the Local Fate of Charge Carriers in Halide Perovskite Thin Films via Correlation Clustering Imaging As the field of metal halide perovskites matures, a range of compositionally different perovskite films has found a place in efficient optoelectronic devices. These films feature variable local struct...

This work was done in @hofkenslab.bsky.social and Martin Vacha's lab and lead by @sudiptaseth.bsky.social.

We combine structural information obtained by SEM with functional information obtained by PL and our correlation clustering imaging.

Here is the link to the paper pubs.acs.org/doi/10.1021/...

Correlative imaging, Functional imaging, photoactive material, Correlation clustering imaging

🔬Interested in imaging in energy science ?🔬

Chemical & Biomedical Imaging, a new journal from @acs.org, invited us for a special issue on

🔬𝐈𝐦𝐚𝐠𝐢𝐧𝐠 𝐢𝐧 𝐄𝐧𝐞𝐫𝐠𝐲 𝐬𝐜𝐢𝐞𝐧𝐜𝐞 ☀️ ⚡ 🧪

And we made the cover ! 🎊

Thanks to @alexeychizhik.bsky.social for the amazing design

#Microscopy #Optics #SciComm

Thanks a lot 😇

The dismantling of US science: can it survive Trump 2.0? Hear the biggest stories from the world of science | 30 April 2025

This week on the Nature Podcast: In the face of massive upheaval, what is the long-term outlook for science in the US and beyond?

https://go.nature.com/4k0pdH6

Hello Prof. Grange!

Would love to be included, here is my google scholar:
scholar.google.com/citations?us...

Also started to post about building of optical setup on this platform.

Hello Mike,

Would love to join if there is still space !
Here is my google scholar: scholar.google.com/citations?us...

Also started to post about microscopy here

Hey, would love to be part of it
Here is my google scholar
scholar.google.com/citations?us...
I am also starting to post regularly on microscopy building and data analysis

Looking forward, will be in U.S. For the first time 🙏

Three-dimensional Optical Reconstruction of colloidal electrokinetics via multiplane imaging Selective manipulation of particles is crucial in many fields, ranging from chemistry to biology and physics. Dielectrophoresis stands out due to its high selectivity potential and the absence of need...

Interested? Check the Arxiv here:
arxiv.org/abs/2503.23839

My talk will focus on 3D mapping of dielectrophoresis and associated electrokinetic flows around quadrupolar electrodes using our 3D multiplane microscope. This is crucial as these forces and flows are important for applications but are equally difficult to characterize and so far unexplored in 3D.

At this point, we are looking into the DEP response of nanoparticles around quadrupolar electrodes, where the idea is to characterize at which range of frequency different nanoparticles are attracted or repelled.

To control the storage of nanoparticles in capillaries, we are using dielectrophoresis as the response of the particles (attraction or repulsion) depends on the frequency of the alternating electric field used.