Jonathan Ready

I also used a new method to quantify very long (2700 bp+) eDNA fragments using duplex ddPCR.

When we combine two mitochondrial assays in duplex, we can use double-positive droplets to quantify the "bridge" between them. Fancy but straightforward stuff.

Wanna know more? ask away :)

Differential Decay of Multiple eNA Components from a Cetacean Environmental nucleic acids (eNA), such as DNA and RNA, are powerful tools for monitoring biodiversity. Yet, interpreting eNA detections requires understanding their environmental persistence—especial...

🔬 Our new preprint is out!

We tracked the decay of multiple #eDNA 🧬& #eRNA molecules from bottlenose dolphins🐬 in seawater.

It may seem like I have caved in for charismatic megafauna. But do not worry. in reality, I am still only interested in their molecules.
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www.biorxiv.org/content/10.1...

eDNA Replicates, Polymerase and Amplicon Size Impact Inference of Richness Across Habitats We conducted a large and controlled experiment, with bulk samples from terrestrial, freshwater and marine environments, to investigate the impacts of natural and technical replicate numbers, PCR poly....

Important info for reporting standards in eDNA... onlinelibrary.wiley.com/doi/full/10....

Comparing the Fate of eDNA by Particle Sizes and Molecule Lengths in Recirculating Streams The detection of environmental DNA (eDNA) has revolutionized aquatic species monitoring, yet interpreting eDNA data remains challenging due to gaps in our understanding of eDNA ecology (i.e., origin,...

New paper alert! 🚨- My final PhD chapter is finally out!

What happens to different eDNA components in water? 🌊💧

We tested particle size (small vs. large) and DNA molecule length (86 vs. 387 bp) in recirculating streams—and what we found might surprise you! 🧵👇

#eDNA #MolecularEcology
🔗 Read more:

@quentinmauvisseau.bsky.social

Geographical variation in mitogenomes of the largetooth sawfish Pristis pristis: challenges and perspectives for conservation efforts Sawfishes (Pristidae) have been severely impacted by coastal development and unregulated fisheries and are considered Critically Endangered by the IUCN Red List. Environmental DNA (eDNA) analyses have...

Local genetic reference sequences can be essential for species specific studies. Applying what worked for a species elsewhere in the world (in this case largetooth sawfish, Pristis pristis) may not work where you are!!! www.biorxiv.org/content/10.1...

Investigating the dynamics of the aquatic community in Oslofjord through time series analysis of eDNA Understanding temporal dynamics of marine communities is critical for assessing ecosystem health and guiding conservation efforts. Here, we conducted a survey using environmental DNA (eDNA) metabarcod...

www.biorxiv.org/content/10.1...

Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America - Scientific Reports Scientific Reports - Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America

Proof that south american squid rings are not pig rectum!!!! doi.org/10.1038/s415...

Please add me!

I'm hiring a coordinator for our #mesophotic Autonomous Reef Monitoring Structures (ARMS) program! This exciting new project will generate unprecedented amounts of information about the biodiversity of deep reefs in the Pacific. Spread the word! us242.dayforcehcm.co...

Check out our new preprint investigating multiple factors that influence variation in outputs of eDNA metabarcoding analyses www.biorxiv.org/content/10.1...