Applied Immunohistochemistry and Molecular Morphology

Comparison of Gastric Signet Ring Cell Carcinoma and Adenocarcinoma From Clinicopathologic Characteristics and Protein Expressions journals.lww.com/appliedimmun...

Investigating Biomarkers in Primary Tumors and Lymph Node... : Applied Immunohistochemistry & Molecular Morphology tases. A total of 60 TSCC biopsy samples, 20 primary metastatic, 20 lymph node metastatic, and 20 nonmetastatic, were immunohistochemically stained to analyze MCM2, CD44, and E-cadherin expression. Ex...

Investigating Biomarkers in Primary Tumors and Lymph Node Metastases in Tongue Squamous Cell Carcinoma: An Immunohistochemical Perspective journals.lww.com/appliedimmun...

Distinct levels of DDIT3 Amplification in Dedifferentiated... : Applied Immunohistochemistry & Molecular Morphology diagnostic tool for accurate diagnosis. While DDIT3 rearrangement is a known characteristic of myxoid LPS, its amplification in DDLPS has not been extensively studied. To investigate this, we evaluate...

Distinct levels of DDIT3 Amplification in Dedifferentiated Liposarcoma Developing After DDIT3 Rearrangement in Myxoid Liposarcoma journals.lww.com/appliedimmun...

STAT6 Can Be Used in the Diagnosis of Hodgkin Lymphoma;... : Applied Immunohistochemistry & Molecular Morphology the STAT6 (EP325) immunostain, diagnosing Hodgkin lymphoma, its staining frequency, immunolocalization, and its relationship with EBV. Sixty-eight patients representing each Hodgkin lymphoma subtype w...

STAT6 Can Be Used in the Diagnosis of Hodgkin Lymphoma; There Are Differences in Expression Among Subtypes journals.lww.com/appliedimmun...

Immunohistochemical Study on Programmed Death Ligand 1... : Applied Immunohistochemistry & Molecular Morphology in order to maximize its therapeutic role in different clinicopathological settings. This study aimed to evaluate PD-L1 immunohistochemical expression in both tumor cells (TCs) and tumor-infiltrating ...

Immunohistochemical Study on Programmed Death Ligand 1 (PD-L1) in Breast Carcinoma and Its Correlation With the Clinicopathological Parameters
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📚 Articles #openaccess starting July 28
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EWSR1::NFATC2 Rearranged Sarcoma: Distinct... : Applied Immunohistochemistry & Molecular Morphology omas with EWSR1–non-ETS fusions, particularly EWSR1::NFATC2 fusion. We identified 5 cases of EWSR1::NFATC2 fusion sarcomas. There was male preponderance and all the patients had a history of trivial t...

EWSR1::NFATC2 Rearranged Sarcoma Distinct Clinicopathological Features From a Series of 5 Rare Cases journals.lww.com/appliedimmun...

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Figure from "Telomerase Immunocytochemistry in Lymphocytes From Chronic Lymphocytic Leukemia” by Sučić, et al.

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Immune Checkpoint Molecule Expression, 9p24.1 Gene... : Applied Immunohistochemistry & Molecular Morphology /refractory cases. This study evaluates the tumor microenvironment (TME), immune checkpoint molecule expression, and the relationship with 9p24.1 gene region alterations in a large cohort of PCNS-LBCL...

Immune Checkpoint Molecule Expression, 9p24.1 Gene Alterations, and Tumor Microenvironment of Primary Central Nervous System Lymphomas and Their Clinical Relevance journals.lww.com/appliedimmun...

Tissue Factor Pathway Inhibitor-2 Expression in Uterine... : Applied Immunohistochemistry & Molecular Morphology cal analyses. In this exploratory study, we conducted an immunohistochemical investigation to determine whether TFPI2 is expressed in cervical cancers, especially CCCC. Further, we examined the expres...

Tissue Factor Pathway Inhibitor-2 Expression in Uterine Cervical Clear Cell Carcinoma: A Potential Biomarker for Clinical Diagnosis journals.lww.com/appliedimmun...

Just published! Check out this latest article by @iamshreeya.bsky.social

🔬 Molecular subtyping of bladder cancer is essential—but not always feasible in low-resource settings
🧪 IHC markers GATA3, KRT20, KRT14, KRT5 used to stratify UCs
⚠️ Dual-negative had poorest survival (25%), luminal best (80%)

Deciphering the Molecular Landscape of Urothelial... : Applied Immunohistochemistry & Molecular Morphology essible antibodies. Examining 100 UCs from 2020 to 2021, encompassing both chemotherapy-naïve MIBC and NMIBC, acquired through transurethral resection or radical resection, we employed IHC with GATA3,...

Deciphering the Molecular Landscape of Urothelial Carcinoma: Immunohistochemistry-based Subtyping Using 4 Easily Available Antibodies for Cost-effective Stratification journals.lww.com/appliedimmun...

The Expression and Clinical Features of 8... : Applied Immunohistochemistry & Molecular Morphology valuable insights into the diagnosis and management of adrenal tumors. We retrospectively analyzed cases of patients treated in the urology department of our hospital from April 2017 to May 2022, focu...

The Expression and Clinical Features of 8 Immunohistochemistry Markers in Adrenal Cortical Adenomas and Pheochromocytomas journals.lww.com/appliedimmun...

Just published! Check out this latest article!
🔵 PD-L1 expression showed high concordance between cores and full slides.
🔵 CD8+ T cell and CD68+ macrophage densities varied; cores underrepresented immune infiltrates.
@fabiotavora.bsky.social #IHCpath #lungcancer

Immune Profiling of Incidental NSCLC Patients: Comparison... : Applied Immunohistochemistry & Molecular Morphology urvival, overall response rate, and progression-free survival in NSCLC patients. This study aimed to evaluate the concordance of PD-L1 expression in NSCLC patients using tissue microarrays (TMA) as pr...

Immune Profiling of Incidental NSCLC Patients: Comparison Between Tumor Sections and TMA Cores journals.lww.com/appliedimmun...

Expression of Hippo Pathway Molecules in Distal Bile Duct... : Applied Immunohistochemistry & Molecular Morphology ) are primary Hippo pathway components. We evaluated LATS1/2, YAP, and TEAD4 expression and their correlation with clinicopathological behavior and prognostic significance in 67 distal bile duct cance...

Expression of Hippo Pathway Molecules in Distal Bile Duct Cancer journals.lww.com/appliedimmun...

Just published! Check out this latest article 👉🏻 HER2 amplification is rare (1.7%) in low-grade, tubule-forming breast cancers with 2+ HER2 IHC; findings suggest ISH may be unnecessary in this subset #breastpath #IHCpath #pathsky

CD140b (PDGFRB) Expression in Atypical... : Applied Immunohistochemistry & Molecular Morphology cing analysis revealed high-level PDGFRA/B gene expression and suggested PDGFRB immunohistochemistry as a “lineage specific” marker for PDS. To evaluate the potential diagnostic utility of PDGFRB stat...

CD140b (PDGFRB) Expression in Atypical Fibroxanthoma/Pleomorphic Dermal Sarcoma and its Cutaneous Neoplastic Mimics journals.lww.com/appliedimmun...

The Value of Routine Reflex In Situ Hybridization Testing... : Applied Immunohistochemistry & Molecular Morphology emistry (IHC). While this recommendation mainly addresses aggressive tumor types such as micropapillary carcinoma, it is applied to low-grade, tubule-forming IBCs unlikely to show HER2 amplification. ...

The Value of Routine Reflex In Situ Hybridization Testing in Low-grade Tubule-forming Invasive Breast Cancers With Equivocal HER2 Immunohistochemistry - A Reappraisal of the ASCO/CAP Guidelines journals.lww.com/appliedimmun...

Evaluation of Diagnostic Accuracy of Immunohistochemical... : Applied Immunohistochemistry & Molecular Morphology ents with mucinous adenocarcinomas—31 lower gastrointestinal (GI), 28 ovarian, and 12 cases of pseudomyxoma peritonei. Immunohistochemistry for SATB2 and villin was performed on ovarian and GI tumor s...

Evaluation of Diagnostic Accuracy of Immunohistochemical Markers of SATB2 and Villin in Differential Diagnosis of Ovarian and Gastrointestinal Mucinous Carcinoma journals.lww.com/appliedimmun...

🧬 Immunohistochemistry in Thymomas
▪️ Bcl-2 and D2-40 positivity varies across thymoma subtypes
▪️ E-cadherin is reduced in invasive tumors
▪️ β-catenin shows subtype-specific expression patterns
📊 66 resected cases analyzed

@byrk_bsr #pathsky #pathology #thymoma

Differential Expression Patterns of Bcl-2, D2-40,... : Applied Immunohistochemistry & Molecular Morphology ymoma biology, but their predictive value for clinical outcomes remains uncertain. This study evaluated the expression of these markers across thymoma subtypes and stages, aiming to assess their progn...

Differential Expression Patterns of Bcl-2, D2-40, β-Catenin, and E-Cadherin in Thymomas: Correlation With Clinical Stages and Subtypes journals.lww.com/appliedimmun...

Telomerase Immunocytochemistry in Lymphocytes From Chronic Lymphocytic Leukemia

✨hTERT shows stronger nuclear staining in B-CLL cells vs. normal; linked to poor B-CLL prognosis #IHCpath #hemepath @katcollmd.bsky.social @ihc-guy.bsky.social
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Figure from "The Prevention of Fatal Tauopathy in a Mouse Model of Alzheimer Disease by Blocking BCL2” by Nuovo, et al.

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Correlation of MammaPrint to Histopathologic Variables and... : Applied Immunohistochemistry & Molecular Morphology 365 ER+/HER2-negative BCs in the adjuvant setting. Further, we analyzed MP and ME results in 26 core biopsy-proven ER+/HER2-negative BCs subjected to neoadjuvant chemotherapy (NACT). Post-NACT respons...

Correlation of MammaPrint to Histopathologic Variables and Comparison to Magee Equations in Adjuvant and Neoadjuvant Cohorts of Estrogen-Receptor Positive HER2-Negative Breast Carcinoma journals.lww.com/appliedimmun...

Coilin Is Not Expressed by Immunohistochemistry in Hodgkin... : Applied Immunohistochemistry & Molecular Morphology To the best of our knowledge, the immunohistochemical expression of coilin has been never investigated in Hodgkin lymphomas. We herein examined, by immunohistochemistry, full tissue sections of 58 cla...

Coilin Is Not Expressed by Immunohistochemistry in Hodgkin and Diffuse Large B-cell Lymphomas
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P53 Puzzle: WWP1 and PARC Immunohistochemistry Illuminate... : Applied Immunohistochemistry & Molecular Morphology e p53 activation, are hypothesized to influence the pathogenesis of serous ovarian tumors. This retrospective study examined 7 low-grade serous carcinomas, 38 high-grade serous carcinomas, and 15 sero...

P53 Puzzle: WWP1 and PARC Immunohistochemistry Illuminate New Pathways for Serous Ovarian Cancer journals.lww.com/appliedimmun...

TAGLN2-mediated Actin Cytoskeleton Stabilization Promotes... : Applied Immunohistochemistry & Molecular Morphology astasis. TAGLN2 expression in ovarian cancer and normal tissues was assessed using Oncomine, Human Protein Atlas (HPA), and immunocytochemistry (IHC). Skov3 cells with TAGLN2 knockdown (transient knoc...

TAGLN2-mediated Actin Cytoskeleton Stabilization Promotes Proliferation and Metastasis of Ovarian Carcinoma journals.lww.com/appliedimmun...

📌TAGLN2 in 84% of ovarian tumors
📌Silencing cuts growth 40%, migration 50%
📌 Binds F-actin; knockdown collapses cytoskeleton
📌Metastasis driver

Expression of EZH2 and Fatty Acid Synthase in Breast... : Applied Immunohistochemistry & Molecular Morphology cer risk factors are unclear. Further, there is a need to characterize healthy breast tissue cohorts for unbiased biomarker evaluation. In this study, we employed the Susan G. Komen healthy volunteer ...

Expression of EZH2 and Fatty Acid Synthase in Breast Tissues From Healthy Women With Breast Cancer Risk Factors
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Toward Accurate Deep Learning-Based Prediction of Ki67, ER, ... : Applied Immunohistochemistry & Molecular Morphology ilt a data set of 185,538 breast cancer (BC) including hematoxylin and eosin (H&E) and associated immunohistochemistry (IHC) images of the proliferative marker Ki67, estrogen receptor (ER), progestero...

Toward Accurate Deep Learning-Based Prediction of Ki67, ER, PR, and HER2 Status From H&E-Stained Breast Cancer Images

🖥️ 185,538 images (H&E + IHC) power a ViT model (90% AUC-ROC)
🔥 AI heat-maps guide progress
📚 Dataset publicly available
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