Matt Foster

Well I do applaud the fact that you are analyzing sub nL of plasma :)

But is data better on an Ultra at low levels for all sample types versus a less sensitive instrument at higher loads?

I think the sds page was used for visualization only. Many more fluids to go, including numerous airway (bronchoalveolaer and nasal lavage, sputum, exhaled condensates) and sex-specific (breast milk, cord blood, amniotic fluid) to name a few.

Nice work. Thoughts on reference pools for whole blood proteomics? Limited to mixing blood from compatible donors, which we have done on a small scale.

We just got a box of those lab coats. So stylish with the cuffs.

All for DIY but then they use commercial ZipTips or HLB for the cleanup 🤔

You can make a multi-course meal with NIST standards. Quenelles de SRM 1946 anyone? Mousse au SRM 2384? Finishing with an SRM 1196, if you're so inclined. @benneely.com can probably tell you how to best prepare the SRM 1945.

NIST PB&J? @benneely.com

I misread those dates 🤣

First decision is desk reject or not.

Huh? If you ingest it but otherwise…..

An Evosep will give you most of the benefits of micro or analytical flow in terms of throughput, minimizing carryover and robustness. Except for low input, what are the benefits of nanoLC?

Still doing 1 mm (100 uL/min) for some neat biofluids where it's easy to digest a ton but I tend agree that input requirements can be limiting. The sweet spot for these systems is really 30+ micrograms on column IMO....

I feel like we just used the keyboard for most games on the Apple 2 and Atari 800. karateka, joust, ….

Also there is a direct connection to QuantumSi in this referenced work. The single molecule counting idea has been around a long time.

Sorry my memory doesn't go back too far but you might trace some of NGS back to this: patents.google.com/patent/US200...

All the original PRM work was done on QEs. Same way we used to do DIA. tMS2 method with inclusion list which includes retention time window and center of m/z window and width (if it’s variable).

You are looking to hire, or collaborate?

Although now I see it has exaggerated the length and direction of some of the PEEK tubing :)

We have an academic license which seems to allow publication. It did pretty good with my spaghetti LC setup :)

99% of lc-ms systems generated in Biorender are an Ultimate with Tribrid so this opens up some new options :)

No one got any of that haha

Haha that was the bot, but it did pull that text from the abstract. Real title might be more impressive: “Live Electrophoresis-Correlative AI Democratizes Single-cell Mass Spectrometry Proteomics”

YouTube video by Shout! Studios The Decline Of Western Civilization | FULL MOVIE | Penelope Spheeris | Music Documentary

At least there's a good soundrack......https://youtu.be/gYK1NGpUZXY?si=eT3OJH-6D9KBF5Fc

NIH scientists say that mRNA vaccines have saved untold thousands of lives. A heroin addled nepo baby whose brain was partially eaten by a worm says mRNA vaccines are worthless and even dangerous. For busy medical patients, it can be hard to know who to trust.

Cue discussion about citing software (unless I missed it) @metamorpheus.bsky.social

Favorite Aussie shows? Mine: “Mr Inbetween” but don’t have a huge sample size.

Predictably after a few weeks of a favorable exchange rate had our most expensive meal (meh; have to get used to tipping again) in the Minneapolis airport with mouse running around the restaurant. A few highlights…

Is it 16,000+ unique protein groups, or just total detected in the two runs?

I have used that term recently. What do you call multi-omics when it's just proteomes (e.g. proteins, glycopeptides, phosphopeptides) and doesn't include transcriptomics/sequencing or metabolomics?